Journal: Journal of diabetes and treatment
Article Title: Exenatide Prevents Diet-induced Hepatocellular Injury in A CEACAM1-Dependent Mechanism
doi:
Figure Lengend Snippet: Effect of exenatide on hepatic fibrosis. A. Liver lysates were analyzed by immunoblotting with α-phospho-Smad2 (α-pSmad2) and α-phospho-Smad3 (α-pSmad3) antibodies followed by reimmuno probing (reIb) with antibodies against total Smad2 and total Smad3, respectively, for normalization. Gels represent more than 2 experiments performed on different mice/feeding/treatment group. B: Liver histology were analyzed by Sirius red staining to detect bridging fibrosis in Cc1−/− mice (n=7–8/genotype/feeding/treatment). Representatives from S-treated RD-fed (i and v), S-treated HF-fed (ii and vi), Ex-treated RD-fed (iii and vii); and Ex-treated HF-fed mice (iv and viii) are shown. The degree of fibrosis was evaluated per Brunt Criteria, and scores are included in the accompanying table.
Article Snippet: In the last month of feeding, mice received an intraperitoneal injection/day of saline or exenatide (20ng/g BW/day) (507-77, California Peptide Research, Salt Lake City, UT) [ 15 ].
Techniques: Western Blot, Staining