derivative  (Echelon Biosciences)

Journal: Journal of diabetes and treatment

Article Title: Exenatide Prevents Diet-induced Hepatocellular Injury in A CEACAM1-Dependent Mechanism

doi:

Figure Lengend Snippet: Effect of exenatide on hepatic ALT and AST activities. Mice were fed with a Regular Diet (RD) or a High-Fat Diet (HF) for 2 months and injected daily with either Saline (S) or Exenatide (Ex) in the last month of feeding. At the end of the feeding/treatment period, AST and ALT activities were measured in duplicate in liver lysates (n=5 mice/genotype/feeding/treatment). RD-S (white), RD-Ex (light grey), HF-S (dark grey), and HF-Ex (black). Values are expressed as mean ± SEM. *P<0.05 HF vs RD/treatment group; †P<0.05 Ex vs S/feeding group; ‡P<0.05 Cc1−/− vs Cc1+/+ mice/feeding group/treatment group.

Article Snippet: In the last month of feeding, mice received an intraperitoneal injection/day of saline or exenatide (20ng/g BW/day) (507-77, California Peptide Research, Salt Lake City, UT) [ 15 ].

Techniques: Injection

Journal: Journal of diabetes and treatment

Article Title: Exenatide Prevents Diet-induced Hepatocellular Injury in A CEACAM1-Dependent Mechanism

doi:

Figure Lengend Snippet: Effect of Exenatide on the mRNA levels of genes in the liver of male mice.

Article Snippet: In the last month of feeding, mice received an intraperitoneal injection/day of saline or exenatide (20ng/g BW/day) (507-77, California Peptide Research, Salt Lake City, UT) [ 15 ].

Techniques:

Journal: Journal of diabetes and treatment

Article Title: Exenatide Prevents Diet-induced Hepatocellular Injury in A CEACAM1-Dependent Mechanism

doi:

Figure Lengend Snippet: Effect of exenatide on hepatic fibrosis. A. Liver lysates were analyzed by immunoblotting with α-phospho-Smad2 (α-pSmad2) and α-phospho-Smad3 (α-pSmad3) antibodies followed by reimmuno probing (reIb) with antibodies against total Smad2 and total Smad3, respectively, for normalization. Gels represent more than 2 experiments performed on different mice/feeding/treatment group. B: Liver histology were analyzed by Sirius red staining to detect bridging fibrosis in Cc1−/− mice (n=7–8/genotype/feeding/treatment). Representatives from S-treated RD-fed (i and v), S-treated HF-fed (ii and vi), Ex-treated RD-fed (iii and vii); and Ex-treated HF-fed mice (iv and viii) are shown. The degree of fibrosis was evaluated per Brunt Criteria, and scores are included in the accompanying table.

Article Snippet: In the last month of feeding, mice received an intraperitoneal injection/day of saline or exenatide (20ng/g BW/day) (507-77, California Peptide Research, Salt Lake City, UT) [ 15 ].

Techniques: Western Blot, Staining