recombinant hsp90α  (BPS Bioscience)

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel PET probe to selectively image heat shock protein 90α/β isoforms in the brain

doi: 10.1186/s41181-024-00248-0

Figure Lengend Snippet: Structures of PET imaging ligands targeting HSP90

Article Snippet: Human recombinant HSP90α and β protein with a His-tag (20 pmol BPS Bioscience, USA) were separately mixed with μMACS Anti-His Tag MicroBeads (50 μL, Miltenyi Biotec, USA) and incubated at 0 °C for 30 min (total volume was 400 μL).

Techniques: Imaging

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel PET probe to selectively image heat shock protein 90α/β isoforms in the brain

doi: 10.1186/s41181-024-00248-0

Figure Lengend Snippet: Binding of [ 11 C]BIIB021 in rat brain homogenates a and human recombinant HSP90α b and HSP90β proteins c . Total (blue circles) and non-specific (red squares) binding of [ 11 C]BIIB021 to a healthy rat brain homogenate, HSP90α and HSP90β. Homologous blocking was performed by 10 μM of nonlabelled BIIB021. The binding by BIIB021 was described by a 1-site model and fitted curves are indicated

Article Snippet: Human recombinant HSP90α and β protein with a His-tag (20 pmol BPS Bioscience, USA) were separately mixed with μMACS Anti-His Tag MicroBeads (50 μL, Miltenyi Biotec, USA) and incubated at 0 °C for 30 min (total volume was 400 μL).

Techniques: Binding Assay, Recombinant, Blocking Assay