covaris me220 focused ultrasonicator  (Covaris)


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    Name:
    ME220 Focused ultrasonicator
    Description:
    multi sample multi application benchtop sample preparation solution
    Catalog Number:
    500506
    Price:
    None
    Size:
    18 75 x 18 75 x 14 75
    Category:
    Instrument
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    1 0
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    Structured Review

    Covaris covaris me220 focused ultrasonicator
    ME220 Focused ultrasonicator
    multi sample multi application benchtop sample preparation solution
    https://www.bioz.com/result/covaris me220 focused ultrasonicator/product/Covaris
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    covaris me220 focused ultrasonicator - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Construct:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Real-time Polymerase Chain Reaction:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Immunoprecipitated DNA and 5% input DNA was analyzed by quantitative real-time PCR using SYBR green mastermix (Qiagen, Copenhagen, Denmark) and primers specific for the satellite II and satellite III regions (for primer sequences, see supplementary materials).

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The pool was further quantitated by quantitative PCR on a CFX Connect real-time system (Bio-Rad, Hercules, CA).

    Incubation:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Equal amounts of sheared chromatin were incubated with 2 μg/μl BSA (Sigma, Aldrich), 1× complete protease inhibitors (Roche, Hvidovre, Denmark) and antibody at 4°C for 3 h, followed by overnight incubation with protein A beads at 4°C, rotating.

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Leaves from 10-d-old B73 seedlings were harvested and incubated for at least 3 h in the enzyme solution. .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Expressing:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: .. Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Equal amounts of sheared chromatin were incubated with 2 μg/μl BSA (Sigma, Aldrich), 1× complete protease inhibitors (Roche, Hvidovre, Denmark) and antibody at 4°C for 3 h, followed by overnight incubation with protein A beads at 4°C, rotating.

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Western Blot:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Transfection:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Immunoprecipitation:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. The antibodies anti-BMI1 (6964; Cell Signaling, 1:50), anti-H3K27me3 (9733; Cell Signaling, 1:50), anti-H2AK119ub (8240; Cell Signaling, 1:100), anti-H3 (4620; Cell Signaling, 1:50), anti-H2A (12349; Cell Signaling, 1:100) and normal rabbit IgG (2729; Cell Signaling, 1:200) were used for immunoprecipitation.

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris). .. Chromatin was immunoprecipitated over night at 4 °C using antibodies and Protein A/G agarose beads (Santa Cruz, sc-2003).

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator. .. GFP-tagged ZmbZIP60 and ZmbZIP17 were immunoprecipitated with anti-GFP antibody (Thermo Fisher Scientific; catalog no. ).

    other:

    Article Title: Polyamino-Isoprenyl Derivatives as Antibiotic Adjuvants and Motility Inhibitors for Bordetella bronchiseptica Porcine Pulmonary Infection Treatment
    Article Snippet: Libraries Construction for Bioinformatic Analysis Libraries were prepared with NebNext kit (New England Biolabs, Ipswich, MA, United States) after Covaris ME220 Covaris Focused-ultrasonicator (Covaris, Woburn, MA, United States) shearing, according to manufacturer protocol, and sequenced on a NextSeq550 (Illumina, San Diego, CA, United States) with High Output 300 cycles V2 cartridge (ref. FC-404-2004).

    Sequencing:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Paragraph title: ChIP Sequencing Analysis ... In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: Paragraph title: Genome sequencing and assembly. ... The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland).

    Sonication:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: .. Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Equal amounts of sheared chromatin were incubated with 2 μg/μl BSA (Sigma, Aldrich), 1× complete protease inhibitors (Roche, Hvidovre, Denmark) and antibody at 4°C for 3 h, followed by overnight incubation with protein A beads at 4°C, rotating.

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: .. Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris). .. Chromatin was immunoprecipitated over night at 4 °C using antibodies and Protein A/G agarose beads (Santa Cruz, sc-2003).

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Fluorescence:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Isolation:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator. .. GFP-tagged ZmbZIP60 and ZmbZIP17 were immunoprecipitated with anti-GFP antibody (Thermo Fisher Scientific; catalog no. ).

    Microscopy:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Purification:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Polymerase Chain Reaction:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    IA:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Chromatin Immunoprecipitation:

    Article Title: IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP
    Article Snippet: .. Chromatin immunoprecipitation Chromatin shearing for ChIP was performed by using the truChIP Chromatin Shearing kit (Covaris #520154) following manufacturer's instructions on a Covaris ME220 focused ultrasonicator. ..

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: Paragraph title: ChIP-qPCR ... Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator.

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: Paragraph title: ChIP ... Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris).

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: Paragraph title: ChIP Sequencing Analysis ... In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Plasmid Preparation:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    SYBR Green Assay:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Immunoprecipitated DNA and 5% input DNA was analyzed by quantitative real-time PCR using SYBR green mastermix (Qiagen, Copenhagen, Denmark) and primers specific for the satellite II and satellite III regions (for primer sequences, see supplementary materials).

    Negative Control:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death [OPEN]
    Article Snippet: The GFP vector alone was used as negative control. .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Agarose Gel Electrophoresis:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. Libraries were pooled in equimolar concentration and size selected on a 2% agarose gel for fragments 500 bp to 800 bp in length.

    Concentration Assay:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. Libraries were pooled in equimolar concentration and size selected on a 2% agarose gel for fragments 500 bp to 800 bp in length.

    Lysis:

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: .. Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris). .. Chromatin was immunoprecipitated over night at 4 °C using antibodies and Protein A/G agarose beads (Santa Cruz, sc-2003).

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  • 95
    Covaris covaris me220 focused ultrasonicator
    Covaris Me220 Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris me220 focused ultrasonicator/product/Covaris
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    covaris me220 focused ultrasonicator - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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    The LE220 plus incorporates automatic water level detection and automatic z height adjustment maintaining the AFA acoustic vessels at a constant optimal distance from the ultrasonic transducer These features control
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