me220 focused ultrasonicatortm  (Covaris)


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    Name:
    ME220 Focused ultrasonicator
    Description:
    multi sample multi application benchtop sample preparation solution
    Catalog Number:
    500506
    Price:
    None
    Size:
    18 75 x 18 75 x 14 75
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris me220 focused ultrasonicatortm
    ME220 Focused ultrasonicator
    multi sample multi application benchtop sample preparation solution
    https://www.bioz.com/result/me220 focused ultrasonicatortm/product/Covaris
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    me220 focused ultrasonicatortm - by Bioz Stars, 2020-08
    95/100 stars

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    Related Articles

    Construct:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    other:

    Article Title: Polyamino-Isoprenyl Derivatives as Antibiotic Adjuvants and Motility Inhibitors for Bordetella bronchiseptica Porcine Pulmonary Infection Treatment
    Article Snippet: Libraries Construction for Bioinformatic Analysis Libraries were prepared with NebNext kit (New England Biolabs, Ipswich, MA, United States) after Covaris ME220 Covaris Focused-ultrasonicator (Covaris, Woburn, MA, United States) shearing, according to manufacturer protocol, and sequenced on a NextSeq550 (Illumina, San Diego, CA, United States) with High Output 300 cycles V2 cartridge (ref. FC-404-2004).

    Expressing:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: .. Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Equal amounts of sheared chromatin were incubated with 2 μg/μl BSA (Sigma, Aldrich), 1× complete protease inhibitors (Roche, Hvidovre, Denmark) and antibody at 4°C for 3 h, followed by overnight incubation with protein A beads at 4°C, rotating.

    Sonication:

    Article Title: Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins
    Article Snippet: .. Briefly, A375 cells with or without ectopic SSX2 expression were crosslinked in 1% formaldehyde and sonicated according to the manufacturer's protocol using the Covaris ME220 Focused-ultrasonicator. .. Equal amounts of sheared chromatin were incubated with 2 μg/μl BSA (Sigma, Aldrich), 1× complete protease inhibitors (Roche, Hvidovre, Denmark) and antibody at 4°C for 3 h, followed by overnight incubation with protein A beads at 4°C, rotating.

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: .. Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris). .. Chromatin was immunoprecipitated over night at 4 °C using antibodies and Protein A/G agarose beads (Santa Cruz, sc-2003).

    Article Title: Reconstructing the Complex Evolutionary History of the Papuasian Schefflera Radiation Through Herbariomics
    Article Snippet: .. Extractions with fragments predominantly above the target insert size (≥ 500 bp) were sonicated with an ME220 Focused-ultrasonicatorTM (Covaris Inc.). .. Genomic Library Preparation We prepared genomic libraries using the NEBNext® UltraTM II DNA Library Prep Kit and Multiplex Oligos (Dual Index Primers, sets 1 and 2) for Illumina® (New England Biolabs) at half-volumes to reduce per sample costs.

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Lysis:

    Article Title: Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver
    Article Snippet: .. Cross-linked cells were washed in PBS, resuspended in lysis buffer (0.1% SDS, 1% Triton X-100, 0.15 M NaCl, 1 mM EDTA, 20 mM TrisHCl [pH 8.0], and BSA 1 mg/ml), and sonicated using Bioruptor (Diagenode) or ME220 Focused-ultrasonicator (Covaris). .. Chromatin was immunoprecipitated over night at 4 °C using antibodies and Protein A/G agarose beads (Santa Cruz, sc-2003).

    Chromatin Immunoprecipitation:

    Article Title: IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP
    Article Snippet: .. Chromatin immunoprecipitation Chromatin shearing for ChIP was performed by using the truChIP Chromatin Shearing kit (Covaris #520154) following manufacturer's instructions on a Covaris ME220 focused ultrasonicator. ..

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  • Bioz Stars
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  • 95
    Covaris me220 focused ultrasonicatortm
    Me220 Focused Ultrasonicatortm, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me220 focused ultrasonicatortm/product/Covaris
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    me220 focused ultrasonicatortm - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

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