me220  (Covaris)


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  • 95
    Name:
    ME220 Focused ultrasonicator
    Description:
    multi sample multi application benchtop sample preparation solution
    Catalog Number:
    500506
    Product Aliases:
    ME220, Ultrasonicator
    Price:
    None
    Size:
    18 75 x 18 75 x 14 75
    Category:
    Instrument
    Quantity:
    1
    Buy from Supplier


    Structured Review

    Covaris me220
    ME220 Focused ultrasonicator
    multi sample multi application benchtop sample preparation solution
    https://www.bioz.com/result/me220/product/Covaris
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    me220 - by Bioz Stars, 2019-09
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Amygdala tissue was dissected and fixed in 1% methanol-free formaldehyde at 37 °C for 5 min and then quenched with 1 M glycine before homogenization in lysis buffer (1% (v/v) SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.0). .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction. .. Aliquots of sonicated chromatin were removed as input for normalization in downstream analysis and the remaining sonicated chromatin was incubated with an antibody to H3K9/14ac (Millipore 06–599; 5 µg/sample) overnight at 4 °C.

    Picogreen Assay:

    Article Title: Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation
    Article Snippet: Fifteen μg of genomic DNA from each of the 22 cocci-shaped Sporosarcina strains were sheared into 400 bp fragments using a Covaris ME220 Focused Acoustic Shearer per the manufacturer’s recommended protocol. .. Barcoded Illumina sequencing libraries were prepared from the sheared fragments using the NEBNext Multiplex Oligos for Illumina (96 Index Primers; New England BioLabs, Ipswich, MA, USA) and NEBNext Ultra II DNA Library Preparation Kit for Illumina (New England BioLabs) following the manufacturer’s instructions.

    Construct:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Electrophoresis:

    Article Title: Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation
    Article Snippet: Fifteen μg of genomic DNA from each of the 22 cocci-shaped Sporosarcina strains were sheared into 400 bp fragments using a Covaris ME220 Focused Acoustic Shearer per the manufacturer’s recommended protocol. .. Barcoded Illumina sequencing libraries were prepared from the sheared fragments using the NEBNext Multiplex Oligos for Illumina (96 Index Primers; New England BioLabs, Ipswich, MA, USA) and NEBNext Ultra II DNA Library Preparation Kit for Illumina (New England BioLabs) following the manufacturer’s instructions.

    Incubation:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Leaves from 10-d-old B73 seedlings were harvested and incubated for at least 3 h in the enzyme solution. .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Expressing:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Western Blot:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Flow Cytometry:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The pool was further quantitated by quantitative PCR on a CFX Connect real-time system (Bio-Rad, Hercules, CA).

    Sequencing:

    Article Title: Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation
    Article Snippet: Paragraph title: Illumina sequencing ... Fifteen μg of genomic DNA from each of the 22 cocci-shaped Sporosarcina strains were sheared into 400 bp fragments using a Covaris ME220 Focused Acoustic Shearer per the manufacturer’s recommended protocol.

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: Paragraph title: Genome sequencing and assembly. ... The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland).

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Paragraph title: ChIP Sequencing Analysis ... In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Sonication:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Amygdala tissue was dissected and fixed in 1% methanol-free formaldehyde at 37 °C for 5 min and then quenched with 1 M glycine before homogenization in lysis buffer (1% (v/v) SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.0). .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction. .. Aliquots of sonicated chromatin were removed as input for normalization in downstream analysis and the remaining sonicated chromatin was incubated with an antibody to H3K9/14ac (Millipore 06–599; 5 µg/sample) overnight at 4 °C.

    Fluorescence:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Isolation:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Three replicates were processed for ChIP analysis performed based on and . .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator. .. GFP-tagged ZmbZIP60 and ZmbZIP17 were immunoprecipitated with anti-GFP antibody (Thermo Fisher Scientific; catalog no. ).

    Transfection:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Microscopy:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Mesophyll protoplasts were transfected with GFP-b60, GFP-b17, and GFP DNA and checked for expression after 24 h by fluorescence microscopy and immunoblots using the GFP antibody ( Invitrogen from Thermo Scientific). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Purification:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction. .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction.

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Chromatin Immunoprecipitation:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Paragraph title: Chromatin immunoprecipitation (ChIP) assay ... Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction.

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: Paragraph title: ChIP Sequencing Analysis ... In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Plasmid Preparation:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: All three constructs were confirmed by sequencing and purified by GenElute High Performance Plasmid Maxiprep Kit (NA-0310; Sigma-Aldrich). .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. Libraries were pooled in equimolar concentration and size selected on a 2% agarose gel for fragments 500 bp to 800 bp in length.

    Negative Control:

    Article Title: Response to Persistent ER Stress in Plants: A Multiphasic Process That Transitions Cells from Prosurvival Activities to Cell Death
    Article Snippet: The GFP vector alone was used as negative control. .. In brief, nuclei were isolated from the frozen protoplasts and chromatin was fragmented ranging from 100 to 600 bp using a Covaris ME220 Focused ultrasonicator.

    Agarose Gel Electrophoresis:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    Homogenization:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Amygdala tissue was dissected and fixed in 1% methanol-free formaldehyde at 37 °C for 5 min and then quenched with 1 M glycine before homogenization in lysis buffer (1% (v/v) SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.0). .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction.

    Concentration Assay:

    Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
    Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

    DNA Purification:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction. .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction.

    Lysis:

    Article Title: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
    Article Snippet: Amygdala tissue was dissected and fixed in 1% methanol-free formaldehyde at 37 °C for 5 min and then quenched with 1 M glycine before homogenization in lysis buffer (1% (v/v) SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.0). .. Homogenate was then sonicated using the Covaris ME220 (Covaris, Woburn, MA, USA) to achieve sheared DNA fragments of 200–500 base pairs which was then clarified using centrifugation (17,000 × g for 10 min, 4 °C) to obtain a chromatin fraction.

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  • 95
    Covaris me220 focused acoustic shearer
    Me220 Focused Acoustic Shearer, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me220 focused acoustic shearer/product/Covaris
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    me220 focused acoustic shearer - by Bioz Stars, 2019-09
    95/100 stars
      Buy from Supplier

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