e220evolution focused ultrasonicator  (Covaris)


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    Name:
    E220evolution Focused ultrasonicator
    Description:
    Option to evolve to E220 Focused ultrasonicator with 96 sample capacity Covaris part 500239UPE Option to evolve to LE220 plus High Throughput Focused ultrasonicator Covaris part 500569UPE
    Catalog Number:
    500429
    Price:
    None
    Size:
    24 W x 30 D x 19 H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris e220evolution focused ultrasonicator
    E220evolution Focused ultrasonicator
    Option to evolve to E220 Focused ultrasonicator with 96 sample capacity Covaris part 500239UPE Option to evolve to LE220 plus High Throughput Focused ultrasonicator Covaris part 500569UPE
    https://www.bioz.com/result/e220evolution focused ultrasonicator/product/Covaris
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    e220evolution focused ultrasonicator - by Bioz Stars, 2020-10
    94/100 stars

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    Related Articles

    Sonication:

    Article Title: The PAX8 cistrome in epithelial ovarian cancer
    Article Snippet: .. Cells were harvested, lysed in a sarkosyl-containing buffer, and sonicated using the Covaris E220evolution Focused-Ultrasonicator. .. 10 μg of an antibody raised against PAX8 (NBP1-32440, Novus) or 5 μg of an antibody raised against lysine 27 acetylated histone 3 (H3K27ac) (C15410196, Diagenode) was incubated with 100 μg and 4ug, respectively, of chromatin at 4°C overnight.

    other:

    Article Title: Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
    Article Snippet: Briefly, for each CIRCLE‐seq reaction, 25 μg of Maize B104 genomic DNA (gDNA) was sheared using an E220evolution (Covaris) to an average length of 300 bp (temperature, 6–7 °C; peak power, 140; duty factor, 10; cycles/burst, 200; duration, 80 s).

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Altered chromatin compaction and histone methylation drive non-additive gene expression in an interspecific Arabidopsis hybrid
    Article Snippet: In brief, chromatin was sheared to an average size of 350 bp with a Covaris E220evolution™ sonicator.

    Article Title: Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
    Article Snippet: DNA was circularized at 5 ng/μL in 100 μL with T4 DNA ligase, and subsequently treated with 50 U Plasmid‐Safe ATP‐dependent DNase (Epicentre) to degrade remaining linear DNA molecules.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: GUIDE-seq libraries were sequenced using an Illumina MiSeq sequencer, and data was analyzed using guideseq v1.1 (ref. ) with an NNNN PAM, a 75 bp window, and allowing up to 9 mismatches prior to downstream data processing ( ).

    Selection:

    Article Title: Convergent evolution of seasonal camouflage in response to reduced snow cover across the snowshoe hare range.
    Article Snippet: .. We sheared genomic DNA to ~300 bp using a Covaris E220evolution ultrasonicator and performed a stringent size selection using a custom-prepared carboxyl-coated magnetic bead mix (Rohland and Reich 2012). .. We determined indexing PCR cycle number for each library with quantitative PCR (qPCR) on a Stratagene Mx3000P thermocycler (Applied Biosystems) using a DyNAmo Flash SYBR Green qPCR kit (Thermo Fisher Scientific).

    Article Title: Convergent evolution of seasonal camouflage in response to reduced snow cover across the snowshoe hare range
    Article Snippet: .. We sheared genomic DNA to ∼300 bp using a Covaris E220evolution ultrasonicator and performed a stringent size selection using a custom-prepared carboxyl-coated magnetic bead mix [ ]. .. We determined indexing PCR cycle number for each library with quantitative PCR (qPCR) on a Stratagene Mx3000P thermocycler (Applied Biosystems) using a DyNAmo Flash SYBR Green qPCR kit (Thermo Fisher Scientific).

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  • 94
    Covaris e220evolution focused ultrasonicator
    E220evolution Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e220evolution focused ultrasonicator/product/Covaris
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e220evolution focused ultrasonicator - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

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