e220evolution focused ultrasonicator  (Covaris)


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    Name:
    E220evolution Focused ultrasonicator
    Description:
    Option to evolve to E220 Focused ultrasonicator with 96 sample capacity Covaris part 500239UPE Option to evolve to LE220 plus High Throughput Focused ultrasonicator Covaris part 500569UPE
    Catalog Number:
    500429
    Price:
    None
    Size:
    24 W x 30 D x 19 H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris e220evolution focused ultrasonicator
    E220evolution Focused ultrasonicator
    Option to evolve to E220 Focused ultrasonicator with 96 sample capacity Covaris part 500239UPE Option to evolve to LE220 plus High Throughput Focused ultrasonicator Covaris part 500569UPE
    https://www.bioz.com/result/e220evolution focused ultrasonicator/product/Covaris
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e220evolution focused ultrasonicator - by Bioz Stars, 2020-02
    93/100 stars

    Related Products / Commonly Used Together

    afa fiber millitube
    sarkosyl-containing buffer

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    Related Articles

    Centrifugation:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    In Vitro:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: For in vitro experiments: Plasmids encoding Cas12a-NLS(nucleoplasmin)-6xHis fusion proteins were transformed into Rosetta 2 (DE3) E. coli and single colonies were inoculated into 25 mL LB medium cultures containing 50 mg/L kanamycin and 25 mg/L chloramphenicol (Kan/Cm) prior to growth at 25 °C for 16 hours. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: For in vitro experiments: Plasmids encoding Cas12a-NLS(nucleoplasmin)-6xHis fusion proteins were transformed into Rosetta 2 (DE3) E. coli and single colonies were inoculated into 25 mL LB medium cultures containing 50 mg/L kanamycin and 25 mg/L chloramphenicol (Kan/Cm) prior to growth at 25 °C for 16 hours. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Expressing:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Paragraph title: Expression and purification of Cas12a proteins. ... The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Paragraph title: Expression and purification of Cas12a proteins. ... The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Ligation:

    Article Title: Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
    Article Snippet: Briefly, for each CIRCLE‐seq reaction, 25 μg of Maize B104 genomic DNA (gDNA) was sheared using an E220evolution (Covaris) to an average length of 300 bp (temperature, 6–7 °C; peak power, 140; duty factor, 10; cycles/burst, 200; duration, 80 s). .. The sheared gDNA was purified with beads and 3 μg was subject to an end‐repair, A‐tailing and adapter ligation protocol for the addition of uracil‐containing stem–loop DNA adaptors (oSQT1288: 5′‐P‐CGGTGGACCGATGATCUATCGGTCCACCG*T‐3′, where * indicates a phosphorothioate linkage) using the KAPA HTP Library Preparation (no amp) Kit (KAPA BioSystems).

    Isolation:

    Article Title: An Examination of Critical Parameters in Hybridization-Based Epigenotyping using Magnetic Microparticles
    Article Snippet: Paragraph title: Isolation of Genomic DNA ... The genomic DNA was sheared using an E220evolution™ focused-ultrasonicator (Covaris) to give a target fragment size of 100 bp.

    Sonication:

    Article Title: The PAX8 cistrome in epithelial ovarian cancer
    Article Snippet: .. Cells were harvested, lysed in a sarkosyl-containing buffer, and sonicated using the Covaris E220evolution Focused-Ultrasonicator. .. 10 μg of an antibody raised against PAX8 (NBP1-32440, Novus) or 5 μg of an antibody raised against lysine 27 acetylated histone 3 (H3K27ac) (C15410196, Diagenode) was incubated with 100 μg and 4ug, respectively, of chromatin at 4°C overnight.

    Purification:

    Article Title: Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
    Article Snippet: All enzymatic steps were purified using paramagnetic beads prepared similar to as previously described (Rohland and Reich, ) [GE Healthcare Sera‐Mag SpeedBeads (Fisher Scientific) washed in 0.1x TE and suspended in 20% PEG‐8000 (w/v), 1.5 m Na Cl, 10 m m Tris‐HCl pH 8, 1 m m EDTA pH 8 and 0.05% Tween20]. .. Briefly, for each CIRCLE‐seq reaction, 25 μg of Maize B104 genomic DNA (gDNA) was sheared using an E220evolution (Covaris) to an average length of 300 bp (temperature, 6–7 °C; peak power, 140; duty factor, 10; cycles/burst, 200; duration, 80 s).

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Paragraph title: Expression and purification of Cas12a proteins. ... The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Paragraph title: Expression and purification of Cas12a proteins. ... The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Incubation:

    Article Title: The PAX8 cistrome in epithelial ovarian cancer
    Article Snippet: Cells were harvested, lysed in a sarkosyl-containing buffer, and sonicated using the Covaris E220evolution Focused-Ultrasonicator. .. 10 μg of an antibody raised against PAX8 (NBP1-32440, Novus) or 5 μg of an antibody raised against lysine 27 acetylated histone 3 (H3K27ac) (C15410196, Diagenode) was incubated with 100 μg and 4ug, respectively, of chromatin at 4°C overnight.

    ChIP-sequencing:

    Article Title: The PAX8 cistrome in epithelial ovarian cancer
    Article Snippet: Paragraph title: Chromatin immunoprecipitation sequencing ... Cells were harvested, lysed in a sarkosyl-containing buffer, and sonicated using the Covaris E220evolution Focused-Ultrasonicator.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. Three sequential elutions were performed with 500 μL elution buffer (20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 10% glycerol, and 500 mM imidazole) and visualized by SDS polyacrylamide gel electrophoresis and coomassie staining.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. Three sequential elutions were performed with 500 μL elution buffer (20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 10% glycerol, and 500 mM imidazole) and visualized by SDS polyacrylamide gel electrophoresis and coomassie staining.

    Protease Inhibitor:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Lysis:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: Cell pellets from 50 mL of culture were harvested by centrifugation at 1200 g for 15 minutes and suspended in 1 mL lysis buffer v1 containing 20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM DTT, Sigmafast protease inhibitor (Sigma-Aldrich), and 0.1% Triton X-100. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Transformation Assay:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: For in vitro experiments: Plasmids encoding Cas12a-NLS(nucleoplasmin)-6xHis fusion proteins were transformed into Rosetta 2 (DE3) E. coli and single colonies were inoculated into 25 mL LB medium cultures containing 50 mg/L kanamycin and 25 mg/L chloramphenicol (Kan/Cm) prior to growth at 25 °C for 16 hours. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: For in vitro experiments: Plasmids encoding Cas12a-NLS(nucleoplasmin)-6xHis fusion proteins were transformed into Rosetta 2 (DE3) E. coli and single colonies were inoculated into 25 mL LB medium cultures containing 50 mg/L kanamycin and 25 mg/L chloramphenicol (Kan/Cm) prior to growth at 25 °C for 16 hours. .. The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C.

    Binding Assay:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. The cell lysate was centrifuged for 20 minutes at 21,000 g and 4 °C, and the supernatant was mixed with an equal volume of binding buffer v1 (lysis buffer v1 with 10 mM imidazole), added to 400 μL of HisPur Ni-NTA Resin (Thermo Fisher Scientific) that was pre-equilibrated in binding buffer v1, and rocked at 4 °C for 8 hours.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. The cell lysate was centrifuged for 20 minutes at 21,000 g and 4 °C, and the supernatant was mixed with an equal volume of binding buffer v1 (lysis buffer v1 with 10 mM imidazole), added to 400 μL of HisPur Ni-NTA Resin (Thermo Fisher Scientific) that was pre-equilibrated in binding buffer v1, and rocked at 4 °C for 8 hours.

    Plasmid Preparation:

    Article Title: Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
    Article Snippet: Briefly, for each CIRCLE‐seq reaction, 25 μg of Maize B104 genomic DNA (gDNA) was sheared using an E220evolution (Covaris) to an average length of 300 bp (temperature, 6–7 °C; peak power, 140; duty factor, 10; cycles/burst, 200; duration, 80 s). .. DNA was circularized at 5 ng/μL in 100 μL with T4 DNA ligase, and subsequently treated with 50 U Plasmid‐Safe ATP‐dependent DNase (Epicentre) to degrade remaining linear DNA molecules.

    Staining:

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. Three sequential elutions were performed with 500 μL elution buffer (20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 10% glycerol, and 500 mM imidazole) and visualized by SDS polyacrylamide gel electrophoresis and coomassie staining.

    Article Title: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
    Article Snippet: The cell suspension was loaded into a 1 mL AFA fiber milliTUBE (Covaris) and was lysed using an E220evolution focused-ultrasonicator (Covaris) according to the following conditions: peak intensity power of 150 W, 200 cycles per burst, duty factor of 10%, and treatment for 20 minutes at 5 °C. .. Three sequential elutions were performed with 500 μL elution buffer (20 mM Hepes pH 7.5, 100 mM KCl, 5 mM MgCl2, 10% glycerol, and 500 mM imidazole) and visualized by SDS polyacrylamide gel electrophoresis and coomassie staining.

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  • 93
    Covaris e220evolution focused ultrasonicator
    E220evolution Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e220evolution focused ultrasonicator/product/Covaris
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e220evolution focused ultrasonicator - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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    Kit Includes 500308 Rack 24 Place microTUBE Screw Cap 520145 microTUBE 15 AFA Beads Screw Cap 25 520059 Centrifuge microTUBE Adaptor 6x16mm 25 500330 microTUBE Prep Station Snap Screw Cap
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