le220 focused ultrasonicator  (Covaris)


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    Name:
    LE220 Focused ultrasonicator
    Description:
    Plate batch or single sample processing includes computer requires recirculating chiller not included
    Catalog Number:
    500219
    Price:
    None
    Size:
    24 W x 30 D x 19 H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris le220 focused ultrasonicator
    LE220 Focused ultrasonicator
    Plate batch or single sample processing includes computer requires recirculating chiller not included
    https://www.bioz.com/result/le220 focused ultrasonicator/product/Covaris
    Average 95 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    le220 focused ultrasonicator - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Integrated genomic analysis of Hürthle cell cancer reveals oncogenic drivers, recurrent mitochondrial mutations, and unique chromosomal landscapes
    Article Snippet: .. DNA was sheared using a LE220 Focused-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR. .. Exome capture was performed using the SureSelect XT v4 51Mb capture probe set (Agilent), and captured exome libraries were enriched by PCR.

    Article Title: Analysis of error profiles in deep next-generation sequencing data
    Article Snippet: Hybridization-capture dataset Genomic DNA was sheared to ~ 150- to 200-bp average size by using a Covaris LE220 focused ultrasonicator. .. The fragmented DNA was then end-repaired, dA-tailed, adapter-ligated, and enriched by PCR amplification using Kapa HTP library preparation kit Illumina 96rxn.

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: A biotin-labeled 5′ PCR primer was used for the amplification of the 5′ cDNA library. .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /).

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA). .. Indexed libraries were amplified by PCR with a limited number of cycles (8 cycles), followed by purification and assessment of the quality and quantity of each library by a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: Sequence library preparation of Quartz-Seq2 We added 130 μL of nuclease-free water including 5–10 ng of amplified cDNA into a Crimp-Cap microTUBE with AFA Fiber. .. We then performed cDNA fragmentation using an LE220 Focused-ultrasonicator (Covaris) under the following conditions: duty factor 15%, peak incident power 450 W, cycles per burst 200, and treatment time 80 s. We purified and concentrated the cDNA solution using the DNA Clean & Concentrator™-5 kit.

    Synthesized:

    Article Title: High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping
    Article Snippet: DNA samples were sheared to a mean size of 600 bp utilizing a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and cleaned with AMPure (Beckman Coulter, Inc., Indianapolis, IN, USA). .. Dual-indexed sequencing libraries were prepared with NEBNext ultra DNA library prep kits for Illumina (New England Biolabs, Ipswich, MA, USA), and barcoding indices were synthesized in-house.

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: The biotin-labeled cDNA libraries were purified and recovered as single-strand cDNAs, and double-strand DNAs were synthesized by KOD-Plus-Ver.2 (TOYOBO; http://www.toyobo-global.com /). .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /).

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: Library preparation and whole metagenome sequencing Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). .. DNA fragments were cleaned with Ampure (Beckman Coulter Inc., Indianapolis, IN) and used to prepare dual-indexed sequencing libraries using the NEBNext Ultra library prep reagents (New England Biolabs Inc., Ipswich, MA), and barcoding indices synthesized at the CDC Biotechnology Core Facility.

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). .. DNA fragments were cleaned with Ampure (Beckman Coulter Inc., Indianapolis, IN) and used to prepare dual-indexed sequencing libraries using the NEBNext Ultra library prep reagents (New England Biolabs Inc., Ipswich, MA), and barcoding indices synthesized at the CDC Biotechnology Core Facility.

    Construct:

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: .. Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: Size of the libraries was confirmed using Fragment Analyzer (Advanced Analytical Technologies), and their concentration was determined by qPCR-based method using a Library quantification kit (KAPA). .. Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies).

    IA:

    Article Title: Detection of structural variation using target captured next-generation sequencing data for genetic diagnostic testing
    Article Snippet: Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA). .. Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA).

    Hybridization:

    Article Title: Analysis of error profiles in deep next-generation sequencing data
    Article Snippet: .. Hybridization-capture dataset Genomic DNA was sheared to ~ 150- to 200-bp average size by using a Covaris LE220 focused ultrasonicator. .. The fragmented DNA was then end-repaired, dA-tailed, adapter-ligated, and enriched by PCR amplification using Kapa HTP library preparation kit Illumina 96rxn.

    Flow Cytometry:

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA). .. Libraries were analyzed for size and concentration, pooled, and denatured for loading onto flow cells for cluster generation.

    Generated:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: .. Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies). .. 1 µg sheared cDNA was taken into library generation, starting at the end repair step, using the TruSeq RNA Sample Preparation kit v2 (Illumina).

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA). .. The conditions used for shearing (duty factor, 15%; peak incident power, 450; treatment time, 490 s) generated DNA fragments with a peak around 150 bp as determined by the high-sensitivity DNA kit and a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Sequence for MD2013 was determined from paired-end reads, which were generated by using an MiSeq apparatus (Illumina, San Diego, CA, USA). .. Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA).

    DNA HS Assay:

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: Purified genomic DNA from fresh beaver blood leukocytes was quantified by Qubit DNA HS Assay (ThermoFisher). .. Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases.

    DNA Sequencing:

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: Paragraph title: Illumina paired-end DNA sequencing: ... Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases.

    Sequencing:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: Paragraph title: RNA-seq library generation and sequencing ... Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies).

    Article Title: High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping
    Article Snippet: Aliquots of DNA were also used for MiSeq sequencing according to the manufacturer’s protocols (Illumina, San Diego, CA, USA). .. DNA samples were sheared to a mean size of 600 bp utilizing a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and cleaned with AMPure (Beckman Coulter, Inc., Indianapolis, IN, USA).

    Article Title: Integrated genomic analysis of Hürthle cell cancer reveals oncogenic drivers, recurrent mitochondrial mutations, and unique chromosomal landscapes
    Article Snippet: .. DNA was sheared using a LE220 Focused-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR. .. Exome capture was performed using the SureSelect XT v4 51Mb capture probe set (Agilent), and captured exome libraries were enriched by PCR.

    Article Title: Genomic dissection of a ‘Fuji’ apple cultivar: re-sequencing, SNP marker development, definition of haplotypes, and QTL detection
    Article Snippet: DNA was fragmented with a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). .. A standard short-read library was built by using a TruSeq DNA Sample Prep v2 Kit and TruSeq SBS v5 kit (Illumina, San Diego, CA, USA) for sequencing runs at 2 × 76 bp total.

    Article Title: Detection of structural variation using target captured next-generation sequencing data for genetic diagnostic testing
    Article Snippet: Paragraph title: NGS library preparation and sequencing ... Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA).

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /). .. Sequencing and data analysis Sequencing was performed by the 454 FLX titanium system (first library; Table ) and the GS FLX+ system (second, third, and fourth libraries; Table ) (Roche; https://roche-biochem.jp/index.html ) according to the manufacturer’s instructions.

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: Illumina paired-end DNA sequencing: We performed whole-genome, short read sequencing at TCAG on the Illumina HiSeq X platform. .. Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases.

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: Paragraph title: Target enrichment library preparation and sequencing. ... Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA).

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: .. Library preparation and whole metagenome sequencing Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). .. DNA fragments were cleaned with Ampure (Beckman Coulter Inc., Indianapolis, IN) and used to prepare dual-indexed sequencing libraries using the NEBNext Ultra library prep reagents (New England Biolabs Inc., Ipswich, MA), and barcoding indices synthesized at the CDC Biotechnology Core Facility.

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: Paragraph title: Library preparation and whole metagenome sequencing ... Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA).

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Paragraph title: Genomic DNA Extraction and Sequencing ... Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA).

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: Paragraph title: Sequence library preparation of Quartz-Seq2 ... We then performed cDNA fragmentation using an LE220 Focused-ultrasonicator (Covaris) under the following conditions: duty factor 15%, peak incident power 450 W, cycles per burst 200, and treatment time 80 s. We purified and concentrated the cDNA solution using the DNA Clean & Concentrator™-5 kit.

    Binding Assay:

    Article Title: High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping
    Article Snippet: Libraries were bound to polymerase using the DNA/polymerase binding kit P5 or P6v2 and were then loaded on single-molecule real-time (SMRT) cells and sequenced with C3 (P5 polymerase) or C4v2 chemistry (P6v2 polymerase) for 270-min (10-kb libraries) or 360-min (20-kb libraries) movies on the RSII instrument (PacBio). .. DNA samples were sheared to a mean size of 600 bp utilizing a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and cleaned with AMPure (Beckman Coulter, Inc., Indianapolis, IN, USA).

    DNA Extraction:

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Paragraph title: Genomic DNA Extraction and Sequencing ... Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA).

    RNA Sequencing Assay:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: Paragraph title: RNA-seq library generation and sequencing ... Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies).

    Article Title: Integrated genomic analysis of Hürthle cell cancer reveals oncogenic drivers, recurrent mitochondrial mutations, and unique chromosomal landscapes
    Article Snippet: For the remaining 7 tumors, from which no paired normal DNA was available, mutations were determined using haplotype caller from RNA-Seq analysis. .. DNA was sheared using a LE220 Focused-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR.

    Isolation:

    Article Title: Detection of structural variation using target captured next-generation sequencing data for genetic diagnostic testing
    Article Snippet: Isolated DNA was quantified using a NanoDrop UV spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and/or Qubit Fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA).

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /). .. Sequencing and data analysis Sequencing was performed by the 454 FLX titanium system (first library; Table ) and the GS FLX+ system (second, third, and fourth libraries; Table ) (Roche; https://roche-biochem.jp/index.html ) according to the manufacturer’s instructions.

    Purification:

    Article Title: Genomic dissection of a ‘Fuji’ apple cultivar: re-sequencing, SNP marker development, definition of haplotypes, and QTL detection
    Article Snippet: DNA was fragmented with a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). .. The size-selected DNA (average length, 300 bp) was purified with gel extraction and AMPure XP (Beckman Coulter, Brea, CA, USA).

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: The biotin-labeled cDNA libraries were purified and recovered as single-strand cDNAs, and double-strand DNAs were synthesized by KOD-Plus-Ver.2 (TOYOBO; http://www.toyobo-global.com /). .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /).

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: Purified genomic DNA from fresh beaver blood leukocytes was quantified by Qubit DNA HS Assay (ThermoFisher). .. Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases.

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA). .. Indexed libraries were amplified by PCR with a limited number of cycles (8 cycles), followed by purification and assessment of the quality and quantity of each library by a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: .. We then performed cDNA fragmentation using an LE220 Focused-ultrasonicator (Covaris) under the following conditions: duty factor 15%, peak incident power 450 W, cycles per burst 200, and treatment time 80 s. We purified and concentrated the cDNA solution using the DNA Clean & Concentrator™-5 kit. .. Purified cDNA was extracted into 10 μL of nuclease-free water from one column purification and transferred into an eight-linked PCR tube (TaKaRa).

    Polymerase Chain Reaction:

    Article Title: Integrated genomic analysis of Hürthle cell cancer reveals oncogenic drivers, recurrent mitochondrial mutations, and unique chromosomal landscapes
    Article Snippet: .. DNA was sheared using a LE220 Focused-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR. .. Exome capture was performed using the SureSelect XT v4 51Mb capture probe set (Agilent), and captured exome libraries were enriched by PCR.

    Article Title: Analysis of error profiles in deep next-generation sequencing data
    Article Snippet: Hybridization-capture dataset Genomic DNA was sheared to ~ 150- to 200-bp average size by using a Covaris LE220 focused ultrasonicator. .. The fragmented DNA was then end-repaired, dA-tailed, adapter-ligated, and enriched by PCR amplification using Kapa HTP library preparation kit Illumina 96rxn.

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: A biotin-labeled 5′ PCR primer was used for the amplification of the 5′ cDNA library. .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /).

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA). .. Indexed libraries were amplified by PCR with a limited number of cycles (8 cycles), followed by purification and assessment of the quality and quantity of each library by a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA). .. DNA fragments were cleaned by using Ampure (Beckman Coulter Inc., Indianapolis, IN, USA) and used to prepare single-indexed sequencing libraries by using the PrepX ILM DNA Library Preparation Kit (Wafergen Biosystems, Fremont, CA, USA), Truseq barcoding indices, and PCR primer cocktail (Illumina).

    Article Title: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
    Article Snippet: We then performed cDNA fragmentation using an LE220 Focused-ultrasonicator (Covaris) under the following conditions: duty factor 15%, peak incident power 450 W, cycles per burst 200, and treatment time 80 s. We purified and concentrated the cDNA solution using the DNA Clean & Concentrator™-5 kit. .. Purified cDNA was extracted into 10 μL of nuclease-free water from one column purification and transferred into an eight-linked PCR tube (TaKaRa).

    cDNA Library Assay:

    Article Title: Generation of expressed sequence tags for discovery of genes responsible for floral traits of Chrysanthemum morifolium by next-generation sequencing technology
    Article Snippet: A biotin-labeled 5′ PCR primer was used for the amplification of the 5′ cDNA library. .. The isolated double-strand DNAs were fragmented to an approximate length of 800 bp and subjected to sequencing analysis by the LE220 Focused-ultrasonicator (Covaris; http://covarisinc.com /).

    Chromatin Immunoprecipitation:

    Article Title: PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies
    Article Snippet: Paragraph title: Comparison of integrated PIXUL-ChIP protocol with commercial Bioruptor and Covaris chromatin shearing instruments followed by Matrix ChIP ... The two best known are the Bioruptor (manufactured by Diagenode), and LE220 Focused Ultrasonicator (manufactured by Covaris).

    Software:

    Article Title: High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping
    Article Snippet: Sequence reads were assembled de novo using the Hierarchical Genome Assembly Process (HGAP3) from the SMRT Analysis Software suite (PacBio) ( ). .. DNA samples were sheared to a mean size of 600 bp utilizing a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and cleaned with AMPure (Beckman Coulter, Inc., Indianapolis, IN, USA).

    Article Title: Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
    Article Snippet: .. Briefly, DNA samples (50 μl in a 96-well microTUBE plate) were sheared using a Covaris LE220 focused ultrasonicator with SonoLab 7.3.2.4 software (Covaris, Inc., Woburn, MA). .. The conditions used for shearing (duty factor, 15%; peak incident power, 450; treatment time, 490 s) generated DNA fragments with a peak around 150 bp as determined by the high-sensitivity DNA kit and a Bioanalyzer 2100 (Agilent Technologies).

    Sample Prep:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: RNA-seq library generation and sequencing For whole-cortex RNA, 1 μg total RNA was used as an input material for library preparation using TruSeq RNA Sample Preparation kit v2 (Illumina). .. Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies).

    Article Title: Genomic dissection of a ‘Fuji’ apple cultivar: re-sequencing, SNP marker development, definition of haplotypes, and QTL detection
    Article Snippet: DNA was fragmented with a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). .. A standard short-read library was built by using a TruSeq DNA Sample Prep v2 Kit and TruSeq SBS v5 kit (Illumina, San Diego, CA, USA) for sequencing runs at 2 × 76 bp total.

    Next-Generation Sequencing:

    Article Title: Detection of structural variation using target captured next-generation sequencing data for genetic diagnostic testing
    Article Snippet: Paragraph title: NGS library preparation and sequencing ... Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA).

    Spectrophotometry:

    Article Title: Detection of structural variation using target captured next-generation sequencing data for genetic diagnostic testing
    Article Snippet: Isolated DNA was quantified using a NanoDrop UV spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and/or Qubit Fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. Genomic DNA was mechanically sheared into 150–500 bp fragments with average size of 300 bp with the LE220 focused ultrasonicator (Covaris, Woburn, MA, USA).

    Produced:

    Article Title: De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)
    Article Snippet: Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA) to an average length between 300 and 400 bases. .. Four lanes of DNA sequences (150 base paired-end reads of a 300–400 base insert) were produced, representing an estimated 160-fold coverage of the genome.

    Concentration Assay:

    Article Title: Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
    Article Snippet: Size of the libraries was confirmed using Fragment Analyzer (Advanced Analytical Technologies), and their concentration was determined by qPCR-based method using a Library quantification kit (KAPA). .. Generated cDNA was sheared using LE220 focused ultrasonicator (Covaris) and quantified by Qubit dsDNA BR Assay (Life Technologies).

    Article Title: High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping
    Article Snippet: DNA samples were sheared to a mean size of 600 bp utilizing a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and cleaned with AMPure (Beckman Coulter, Inc., Indianapolis, IN, USA). .. The resulting libraries were analyzed for size and concentration, pooled, and denatured for loading onto a flowcell for cluster generation.

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: Library preparation and whole metagenome sequencing Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). .. Libraries were analyzed for size and concentration, pooled and denatured for loading onto flowcells for cluster generation.

    Article Title: Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors
    Article Snippet: Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). .. Libraries were analyzed for size and concentration, pooled and denatured for loading onto flowcells for cluster generation.

    Article Title: Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere
    Article Snippet: Genomic DNA was sheared to a mean size of 600 bp by using an LE220 focused ultrasonicator (Covaris Inc., Woburn, MA, USA). .. Libraries were analyzed for size and concentration, pooled, and denatured for loading onto flow cells for cluster generation.

    Gel Extraction:

    Article Title: Genomic dissection of a ‘Fuji’ apple cultivar: re-sequencing, SNP marker development, definition of haplotypes, and QTL detection
    Article Snippet: DNA was fragmented with a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). .. The size-selected DNA (average length, 300 bp) was purified with gel extraction and AMPure XP (Beckman Coulter, Brea, CA, USA).

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  • 95
    Covaris le220 focused ultrasonicator
    Le220 Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/le220 focused ultrasonicator/product/Covaris
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    le220 focused ultrasonicator - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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    g TUBE is a single use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb The only equipment needed is a compatible bench top centrifuge
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