cryoprep pulverizer  (Covaris)


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    Name:
    CP01 Cryoprep Manual Pulverizer
    Description:
    The CP03 Cryoprep manual pulverizer can be used to cryofracture frozen tissues within a mass range of 2 to 10g
    Catalog Number:
    500230
    Price:
    None
    Category:
    Instrument
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    1 0
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    Structured Review

    Covaris cryoprep pulverizer
    CP01 Cryoprep Manual Pulverizer
    The CP03 Cryoprep manual pulverizer can be used to cryofracture frozen tissues within a mass range of 2 to 10g
    https://www.bioz.com/result/cryoprep pulverizer/product/Covaris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryoprep pulverizer - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Protein Extraction:

    Article Title: Systems biology driving drug development: from design to the clinical testing of the anti-ErbB3 antibody seribantumab (MM-121)
    Article Snippet: Enzyme-linked immunosorbent assay To study intracellular signaling, cell lysates were prepared and ELISAs were performed as previously described. .. To generate tumor lysates, flash frozen tumors were pulverized in a CryoPrep pulverizer (Covaris, Woburn, MA, USA) and resuspended in T-PER Tissue Protein Extraction Buffer (Thermo Scientific, Tewksbury, MA, USA), supplemented with protease and proteinase inhibitors (Roche, Indianapolis, IN). .. HRG was detected by indirect ELISA as previously described.

    Mouse Assay:

    Article Title: Stress keratin 17 enhances papillomavirus infection-induced disease by downregulating T cell recruitment
    Article Snippet: .. Fresh papillomas or mock-infected ear tissues from FVB/N mice were rinsed with RNase inhibitor, snap frozen in liquid nitrogen, placed into tissueTUBE (Covaris) and pulverized by Cryoprep Pulverizer (Covaris). .. Total RNA was isolated by addition of 1ml or TRizol (Thermo Fisher Scientific) using RNA-binding columns (Qiagen RNA isolation kit).

    Transferring:

    Article Title: Disease-associated short tandem repeats co-localize with chromatin domain boundaries
    Article Snippet: Libraries were sequenced on Illumina NextSeq 500 according to the manufacturer’s instruction. .. Flash frozen human tissue was first pulverized using the CP01 Cryoprep Manual Pulverizer by transferring 20 mg of frozen tissue into Covaris TT1 tissue tubes, placing the tube in the Cryoprep Pulverizer, and hitting with a hammer 5 times. ..

    Lysis:

    Article Title: Electron Microscopic and Proteomic Comparison of Terminal Branches of the Trigeminal Nerve in Patients with and without Migraine Headaches
    Article Snippet: .. Frozen nerve tissue samples ( n = 5 per group for the original study and n = 10 per group for the follow-up study) were pulverized using a cryoPREP pulverizer (Covaris, Inc., Woburn, Mass.) and homogenized in lysis buffer (50 mM Tris-Hcl, pH 7.5, 4% sodium dodecyl sulfate, 40 mM dithiothreitol, and protease inhibitor cocktail). .. Lysates were reduced, alkylated, and fractionated using 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Protease Inhibitor:

    Article Title: Electron Microscopic and Proteomic Comparison of Terminal Branches of the Trigeminal Nerve in Patients with and without Migraine Headaches
    Article Snippet: .. Frozen nerve tissue samples ( n = 5 per group for the original study and n = 10 per group for the follow-up study) were pulverized using a cryoPREP pulverizer (Covaris, Inc., Woburn, Mass.) and homogenized in lysis buffer (50 mM Tris-Hcl, pH 7.5, 4% sodium dodecyl sulfate, 40 mM dithiothreitol, and protease inhibitor cocktail). .. Lysates were reduced, alkylated, and fractionated using 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Synthesized:

    Article Title: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice
    Article Snippet: Reverse Transcription–Polymerase Chain Reaction The tissues harvested from mice were flash frozen in liquid nitrogen and stored at −80°C. .. To prepare RNA, the frozen tissue was pulverized using Cryoprep System (#CP-01; Covaris, city, state, country), and the powder was lysed for RNA preparation following the instruction of RNeasy Mini Kit (#74106; Qiagen, Germantown, MD, USA). cDNA was synthesized using Invitrogen SuperScript III First-Strand Synthesis System (#18080051; ThermoFisher Scientific). .. The PCR reaction mixture was prepared as mentioned in the Mouse genotyping section.

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  • 93
    Covaris cryoprep pulverizer
    Cryoprep Pulverizer, supplied by Covaris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryoprep pulverizer/product/Covaris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryoprep pulverizer - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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