covaris s220 sonicator  (Covaris)


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    Name:
    S220 Focused ultrasonicator
    Description:
    High performance Ultrasonicator for samples of mass 1000mg and or volume 10ml Single sample preparation system serial process Includes computer and SonoLab software
    Catalog Number:
    500217
    Price:
    None
    Category:
    Instrument
    Size:
    8 W x 21 D x 13 H
    Quantity:
    1 0
    Buy from Supplier


    Structured Review

    Covaris covaris s220 sonicator
    S220 Focused ultrasonicator
    High performance Ultrasonicator for samples of mass 1000mg and or volume 10ml Single sample preparation system serial process Includes computer and SonoLab software
    https://www.bioz.com/result/covaris s220 sonicator/product/Covaris
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    covaris s220 sonicator - by Bioz Stars, 2021-05
    99/100 stars

    Images

    1) Product Images from "Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)"

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-4939-7768-0_12

    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    Figure Legend Snippet: Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Techniques Used: Chromatin Immunoprecipitation, Labeling

    2) Product Images from "Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)"

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-4939-7768-0_12

    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    Figure Legend Snippet: Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Techniques Used: Chromatin Immunoprecipitation, Labeling

    Related Articles

    Sequencing:

    Article Title: Exploration of deep terrestrial subsurface microbiome in Late Cretaceous Deccan traps and underlying Archean basement, India
    Article Snippet: After multiple DNA extraction, concentrations of DNA were determined by Quant-iT Picogreen dsDNA assay. .. DNA samples were fragmented to ~250 base pairs (bp) using a Covaris S220 Focused-ultrasonicator (Covaris Inc. Woburn, MA) and paired end (2 × 151 bp) sequencing was performed at Marine Biological laboratory, Woods Hole, USA using Illumina NextSeq500 platform under Deep Carbon Observatory (DCO), Census of Deep Life program. .. Details of metagenomic library preparation and sequencing are mentioned in the supplementary information.

    Size-exclusion Chromatography:

    Article Title: Base-Resolution Analysis of DNA Methylation Patterns Downstream of Dnmt3a in Mouse Naïve B Cells
    Article Snippet: Quantification of DNA during library production was performed using Qubit dsDNA Assay Kits (Invitrogen). .. For each sample, 1500 ng intact genomic DNA and 1.5 ng unmethylated λ DNA (Promega) were added to a microTUBE (Covaris) and fragmented using a Covaris S220 focused ultrasonicator according to the manufacturer’s instructions, with the following settings: target BP (peak), 300; peak incident power (W), 175; duty factor, 10%; cycles per burst, 200; treatment time (sec), 50; temperature (°C), 7; water level, 12; and sample volume (microliter), 50. .. Sequencing libraries were prepared using the NEXTflex Bisulfite-Seq Kit (BIOO Scientific) with a starting input of 1 μg.

    Sonication:

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)
    Article Snippet: Note 4 In addition to the phenol chloroform extraction method, other methods of isolating human genomic DNA may be used, such as the column-based genomic DNA isolation kit from Zymo (Genomic DNA Clean & Concentrator −25). .. Note 5 If using the Covaris S220 sonicator, no more than 10 μg of DNA should be fragmented at a time; if you obtained more than 10 μg of DNA from the treated cells, you should dilute to 100 ng/ul and only use 8–10 μg per sonication tube. .. Note 6 Sonication must be optimized for each cell type to produce 100–200 bp fragments.

    Article Title: A comprehensive epigenome atlas reveals DNA methylation regulating skeletal muscle development
    Article Snippet: Following whole genome bisulfite sequencing (WGBS), sex-calls were further confirmed using the ratio of mapped reads in the X chromosome to the Y chromosome. .. Library constructionTotal DNA for WGBS was extracted and fragmented by sonication to 200−300 bp using a Covaris S220 (Covaris, Woburn, MA, USA), followed by end repair and A-ligation. .. After ligation to cytosine-methylated barcodes, the DNA fragments were treated twice with bisulfite using an EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA).

    Article Title: Nuclear Arp2/3 drives DNA break clustering for homology-directed repair
    Article Snippet: Cell lysis and nuclear isolation were performed using NP40 lysis buffer and SDS shearing buffer (Covaris). .. Nuclei were sonicated using the S220 Ultrasonicator (Covaris) to obtain chromatin fragments of 500 – 1000 bp in length. .. Sheared chromatin was incubated with 10 μg antibodies to Rad51 (Santa Cruz: sc-8349), DNA-PKcs (Abcam: ab1832), WASP (Santa Cruz: sc-5300), Arpc2 (Santa Cruz: sc-32195) or IgG (Jackson ImmunoResearch Laboratories) overnight.

    Construct:

    Article Title: Genome Evolution of Bartonellaceae Symbionts of Ants at the Opposite Ends of the Trophic Scale
    Article Snippet: .. Libraries were constructed using the KAPA low-throughput Illumina library preparation kit from extracted DNA sheared on a Covaris S220 sonicator. .. The libraries were amplified with the KAPA HiFi amplification kit, quality assessed on an Agilent Bioanalyzer, pooled, and sequenced on an Illumina HiSeq2500 instrument at the Harvard Bauer Core Facility.

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    Covaris covaris s220 sonicator
    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a <t>Covaris</t> <t>S220</t> sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    Covaris S220 Sonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris s220 sonicator/product/Covaris
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    covaris s220 sonicator - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    doi: 10.1007/978-1-4939-7768-0_12

    Figure Lengend Snippet: Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Article Snippet: If using a Covaris S220 sonicator, it is recommended that you start by using a 10% duty cycle, an intensity setting of 5, and 200 cycles per burst for 6 minutes.

    Techniques: Chromatin Immunoprecipitation, Labeling