covaris s220 ultrasonicator  (Covaris)


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    Name:
    S220 Focused ultrasonicator
    Description:
    High performance Ultrasonicator for samples of mass 1000mg and or volume 10ml Single sample preparation system serial process Includes computer and SonoLab software
    Catalog Number:
    500217
    Price:
    None
    Size:
    8 W x 21 D x 13 H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris covaris s220 ultrasonicator
    S220 Focused ultrasonicator
    High performance Ultrasonicator for samples of mass 1000mg and or volume 10ml Single sample preparation system serial process Includes computer and SonoLab software
    https://www.bioz.com/result/covaris s220 ultrasonicator/product/Covaris
    Average 99 stars, based on 151 article reviews
    Price from $9.99 to $1999.99
    covaris s220 ultrasonicator - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages
    Article Snippet: .. For CDK9, nuclei were sonicated with Covaris S220 Ultrasonicator according to manufacturer’s instructions (130 μl shearing buffer, 200 cycles/burst, 120 s, DF 10). .. Lysates were cleared by centrifugation at 14,000*g, 20 min, 4°C, and then incubated with normal rabbit IgG (Santa Cruz Biotech, sc-2027x), BRD4 (Abcam, ab84776 and Bethyl Laboratories, A3001-985A100), MED12 (Bethyl Laboratories, A300-774A), MED1 (Bethyl Laboratories, A300-793), p300 (Santa Cruz Biotech, sc-585X), Anti-AcH4 (Millipore, 06–866), Anti-AcH4K12 (Millipore, 07–595), Anti-AcH4K5 (Millipore, 07–327) and 40 μl of 50% protein A/G plus agarose (Santa Cruz Biotech, sc-2003) per reaction at 4°C ON.

    Article Title: Loss of Tet enzymes compromises proper differentiation of embryonic stem cells
    Article Snippet: .. 2 μg of genomic DNA isolated from day 10 EBs was sonicated to 300bp fragments using a Covaris S220 ultrasonicator (Covaris). .. One μg of purified adapter ligated DNA was used for pooled immunoprecipitations using a polyclonal 5mC-specific antibody (Active Motif, 39791).

    Purification:

    Article Title: Functional and evolutionary insights from the Ciona notochord transcriptome
    Article Snippet: .. Purified RNA (2.5 μl) was used in half-reactions following their standard protocol. cDNA samples were fragmented with a Covaris S220 Ultrasonicator using the recommended settings to obtain an average fragment length of 300 bp and the NEBNext DNA Library Prep Master Mix set for Illumina from New England BioLabs (NEB) was used for library construction. .. The resulting libraries were quality checked with an Agilent 2100 Bioanalyzer and quantified by RT-PCR.

    Article Title: Pancreatic islet chromatin accessibility and conformation reveals distal enhancer networks of type 2 diabetes risk
    Article Snippet: .. DNA was purified with phenol-chloroform extraction and ethanol precipitation, followed by fragmentation to 300–500 bp with the Covaris S220 ultrasonicator. .. Ligation products were enriched with Dynabeads My One T1 Streptavidin beads (Life Technologies).

    Isolation:

    Article Title: Loss of Tet enzymes compromises proper differentiation of embryonic stem cells
    Article Snippet: .. 2 μg of genomic DNA isolated from day 10 EBs was sonicated to 300bp fragments using a Covaris S220 ultrasonicator (Covaris). .. One μg of purified adapter ligated DNA was used for pooled immunoprecipitations using a polyclonal 5mC-specific antibody (Active Motif, 39791).

    Ethanol Precipitation:

    Article Title: Pancreatic islet chromatin accessibility and conformation reveals distal enhancer networks of type 2 diabetes risk
    Article Snippet: .. DNA was purified with phenol-chloroform extraction and ethanol precipitation, followed by fragmentation to 300–500 bp with the Covaris S220 ultrasonicator. .. Ligation products were enriched with Dynabeads My One T1 Streptavidin beads (Life Technologies).

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  • 99
    Covaris covaris s220 ultrasonicator
    Covaris S220 Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris s220 ultrasonicator/product/Covaris
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    covaris s220 ultrasonicator - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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