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Illumina Inc 500 550 mid output v2
500 550 Mid Output V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/500 550 mid output v2/product/Illumina Inc
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
500 550 mid output v2 - by Bioz Stars, 2019-12
88/100 stars

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SYBR Green Assay:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: The 10 μL qPCR reaction mix contained 1 μL of diluted library and final concentrations of 1 × SYBR Green qPCR Master Mix (Applied Biosystems 4309155) and 0.2 μM of each primer IS7 and IS8 [ ]. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer.

Flow Cytometry:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: Final concentrations and PCR were as described by Gansauge and Meyer [ ], but using 20 μL of template DNA in a total reaction volume of 80 μL. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer. .. Although paired-end data was acquired for some libraries (GP1-01, GP1-02, GP1-03), only their first reads were used in data analysis, effectively unifying all sequence reads to single-end data of 76 bp length.

Amplification:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: A quantitative PCR (qPCR) experiment was carried out using 0.2% of the unamplified library to estimate relative library complexities (Additional file : Table S1), and to determine the optimal number of cycles for subsequent indexing PCR, representing the inflection point of the respective library amplification curves, corrected for reaction volume and template amount. qPCR was performed on a PikoReal 96 Real-Time PCR machine (Thermo Fisher Scientific TCR0096) with 3 replicates for each library, involving an initial 10 min denaturation at 95 °C, followed by 40 cycles of: 15 s at 95 °C, 30 s at 60 °C, and 1 min at 72 °C. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer.

Ligation:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: 2.5 U/μL of Circligase II (Biozym 131406) was used and the ligation reaction carried out overnight. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer.

Real-time Polymerase Chain Reaction:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: The 10 μL qPCR reaction mix contained 1 μL of diluted library and final concentrations of 1 × SYBR Green qPCR Master Mix (Applied Biosystems 4309155) and 0.2 μM of each primer IS7 and IS8 [ ]. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer.

Sequencing:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: Final concentrations and PCR were as described by Gansauge and Meyer [ ], but using 20 μL of template DNA in a total reaction volume of 80 μL. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer. .. Although paired-end data was acquired for some libraries (GP1-01, GP1-02, GP1-03), only their first reads were used in data analysis, effectively unifying all sequence reads to single-end data of 76 bp length.

DNA Sequencing:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: Final concentrations and PCR were as described by Gansauge and Meyer [ ], but using 20 μL of template DNA in a total reaction volume of 80 μL. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer. .. Although paired-end data was acquired for some libraries (GP1-01, GP1-02, GP1-03), only their first reads were used in data analysis, effectively unifying all sequence reads to single-end data of 76 bp length.

Polymerase Chain Reaction:

Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
Article Snippet: Final concentrations and PCR were as described by Gansauge and Meyer [ ], but using 20 μL of template DNA in a total reaction volume of 80 μL. .. DNA sequencing was performed on an Illumina NextSeq 500 sequencing platform, using 500/550 Mid Output v2 (150 cycles, Illumina FC-404-2001) and 500/550 High Output v2 (75 cycles, Illumina FC-404-2005) kits, with a custom read-1 [ ] and a custom index-2 [ ] sequencing primer.

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    Illumina Inc 500 550 mid output v2 sequencing kit
    500 550 Mid Output V2 Sequencing Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/500 550 mid output v2 sequencing kit/product/Illumina Inc
    Average 91 stars, based on 2 article reviews
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    Illumina Inc nextseq 500 550 mid output kit v2 chemistry
    Nextseq 500 550 Mid Output Kit V2 Chemistry, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc nextseq 500 550 mid high output v1 v2 kits
    Nextseq 500 550 Mid High Output V1 V2 Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextseq 500 550 mid high output v1 v2 kits/product/Illumina Inc
    Average 77 stars, based on 1 article reviews
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    nextseq 500 550 mid high output v1 v2 kits - by Bioz Stars, 2019-12
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