50 nm silencer select control small interfering rna  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Stealth RNAi siRNA Negative Control Med GC Duplex 2
    Description:
    Stealth RNAi siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi siRNA duplex versus background These controls are • Designed in 3 levels of GC content Lo Med Hi with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi siRNA• Not homologous to anything in the vertebrate transcriptome• Tested not to induce stress response Stealth RNAi siRNANegative Control Kit contains all three controls Lo Med Hi GC content excluding sequences 2 and 3 and each is also available separately Get the ideal negative controls for your RNAi experiment so you can assess your results accurately
    Catalog Number:
    12935112
    Price:
    None
    Applications:
    Cell Culture|RNAi|RNAi Transfection|RNAi, Epigenetics & Non-Coding RNA Research|Synthetic siRNA Transfection|siRNA|Transfection
    Category:
    Standards Ladders Controls
    Buy from Supplier


    Structured Review

    Thermo Fisher 50 nm silencer select control small interfering rna
    Stealth RNAi siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi siRNA duplex versus background These controls are • Designed in 3 levels of GC content Lo Med Hi with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi siRNA• Not homologous to anything in the vertebrate transcriptome• Tested not to induce stress response Stealth RNAi siRNANegative Control Kit contains all three controls Lo Med Hi GC content excluding sequences 2 and 3 and each is also available separately Get the ideal negative controls for your RNAi experiment so you can assess your results accurately
    https://www.bioz.com/result/50 nm silencer select control small interfering rna/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    50 nm silencer select control small interfering rna - by Bioz Stars, 2020-09
    95/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress
    Article Snippet: .. Transfection and infection For siRNA transfection as described previously [ ], cells were reversely transfected with RNA iMAX (Life Technologies; 13778–150) and 5 nM scramble siRNA (Life Technologies, 12935–112), kinome siRNA library (2127 siRNA for 709 gene, A30079, Thermo Fisher Scientific), ATG5 (GE Healthcare Dharmacon, 9474), ATG7 (Life Technologies, s20652), BECN1 (Life Technologies, 4392420), ULK1 (Dharmacon, 8408), or ERBB2 (Life Technologies, 4390824;) for 72 h. For shRNA infection, 8XARE reporter plasmid, a packaging and VSV-G expressing envelope plasmid, were transfected into HEK293T cells using the transfection reagent, Lipofectamine 2000 (Life Technologies, 11668–027) for two days to achieve an infection. ..

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Article Title: Protocadherin-1 is a glucocorticoid-responsive critical regulator of airway epithelial barrier function
    Article Snippet: .. siRNA transfection 16HBE cells were grown in six-well plates to 50 % confluence and transfected individually with either 50-nM Silencer Select Control small interfering RNA (siCtlRNA, cat. 12935–112; Invitrogen, Carlsbad, CA, USA) or human PCDH1 siRNAs (siPCDH1_1, siPCDH1_2, and siPCDH1_3, all obtained from Sigma-Aldrich) for 24 h using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions. .. The transfected cells were seeded on Transwell chambers (Corning Life Sciences, Corning, NY, USA) before replacing the transfection medium with complete medium with or without Dex.

    Article Title: Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells
    Article Snippet: .. Transfection and shRNA infection For siRNA transfection, the cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies; 13778–150) in the presence of 10 nM scrambled siRNA (Life Technologies, 12935–112), siRNA against ATG5 (GE Healthcare Dharmacon, City, Country, 9474) or ATG7 (Life Technologies, s20652) or BECN1 (Life Technologies, 4392420) for 72 h. For shRNA infection, shRNAs against ATG5 (TRCN0000151963, The RNAi Consortium, Taiwan), ATG7 (TRCN0000007584, The RNAi Consortium, Taiwan), and pLKO.1 Scrambled shRNA (Addgene, 1864). .. The plasmids were transfected into HEK293FT cells with Lipofectamine 2000 (Life Technologies, 11668–027) for 2 days, and the supernatant were used to infect ARPE-19 cells.

    shRNA:

    Article Title: ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress
    Article Snippet: .. Transfection and infection For siRNA transfection as described previously [ ], cells were reversely transfected with RNA iMAX (Life Technologies; 13778–150) and 5 nM scramble siRNA (Life Technologies, 12935–112), kinome siRNA library (2127 siRNA for 709 gene, A30079, Thermo Fisher Scientific), ATG5 (GE Healthcare Dharmacon, 9474), ATG7 (Life Technologies, s20652), BECN1 (Life Technologies, 4392420), ULK1 (Dharmacon, 8408), or ERBB2 (Life Technologies, 4390824;) for 72 h. For shRNA infection, 8XARE reporter plasmid, a packaging and VSV-G expressing envelope plasmid, were transfected into HEK293T cells using the transfection reagent, Lipofectamine 2000 (Life Technologies, 11668–027) for two days to achieve an infection. ..

    Article Title: Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells
    Article Snippet: .. Transfection and shRNA infection For siRNA transfection, the cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies; 13778–150) in the presence of 10 nM scrambled siRNA (Life Technologies, 12935–112), siRNA against ATG5 (GE Healthcare Dharmacon, City, Country, 9474) or ATG7 (Life Technologies, s20652) or BECN1 (Life Technologies, 4392420) for 72 h. For shRNA infection, shRNAs against ATG5 (TRCN0000151963, The RNAi Consortium, Taiwan), ATG7 (TRCN0000007584, The RNAi Consortium, Taiwan), and pLKO.1 Scrambled shRNA (Addgene, 1864). .. The plasmids were transfected into HEK293FT cells with Lipofectamine 2000 (Life Technologies, 11668–027) for 2 days, and the supernatant were used to infect ARPE-19 cells.

    Cell Cycle Assay:

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Cytometry:

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Infection:

    Article Title: ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress
    Article Snippet: .. Transfection and infection For siRNA transfection as described previously [ ], cells were reversely transfected with RNA iMAX (Life Technologies; 13778–150) and 5 nM scramble siRNA (Life Technologies, 12935–112), kinome siRNA library (2127 siRNA for 709 gene, A30079, Thermo Fisher Scientific), ATG5 (GE Healthcare Dharmacon, 9474), ATG7 (Life Technologies, s20652), BECN1 (Life Technologies, 4392420), ULK1 (Dharmacon, 8408), or ERBB2 (Life Technologies, 4390824;) for 72 h. For shRNA infection, 8XARE reporter plasmid, a packaging and VSV-G expressing envelope plasmid, were transfected into HEK293T cells using the transfection reagent, Lipofectamine 2000 (Life Technologies, 11668–027) for two days to achieve an infection. ..

    Article Title: Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells
    Article Snippet: .. Transfection and shRNA infection For siRNA transfection, the cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies; 13778–150) in the presence of 10 nM scrambled siRNA (Life Technologies, 12935–112), siRNA against ATG5 (GE Healthcare Dharmacon, City, Country, 9474) or ATG7 (Life Technologies, s20652) or BECN1 (Life Technologies, 4392420) for 72 h. For shRNA infection, shRNAs against ATG5 (TRCN0000151963, The RNAi Consortium, Taiwan), ATG7 (TRCN0000007584, The RNAi Consortium, Taiwan), and pLKO.1 Scrambled shRNA (Addgene, 1864). .. The plasmids were transfected into HEK293FT cells with Lipofectamine 2000 (Life Technologies, 11668–027) for 2 days, and the supernatant were used to infect ARPE-19 cells.

    Small Interfering RNA:

    Article Title: Protocadherin-1 is a glucocorticoid-responsive critical regulator of airway epithelial barrier function
    Article Snippet: .. siRNA transfection 16HBE cells were grown in six-well plates to 50 % confluence and transfected individually with either 50-nM Silencer Select Control small interfering RNA (siCtlRNA, cat. 12935–112; Invitrogen, Carlsbad, CA, USA) or human PCDH1 siRNAs (siPCDH1_1, siPCDH1_2, and siPCDH1_3, all obtained from Sigma-Aldrich) for 24 h using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions. .. The transfected cells were seeded on Transwell chambers (Corning Life Sciences, Corning, NY, USA) before replacing the transfection medium with complete medium with or without Dex.

    Negative Control:

    Article Title: DAAM1 stabilizes epithelial junctions by restraining WAVE complex–dependent lateral membrane motility
    Article Snippet: .. We used the Stealth RNAi siRNA Negative Control Medium GC Duplex #2 (12935-112; Invitrogen) as a control. .. For stable shRNA expression, a DNA oligo for DAAM1 siRNA#1 sequence was inserted into the pBAsi-mU6-PUR vector (Takara Bio Inc.).

    Article Title: The Rho guanine nucleotide exchange factor ARHGEF5 promotes tumor malignancy via epithelial–mesenchymal transition
    Article Snippet: .. The series of ARHGEF5 siRNA duplexes (siGEF5 no. 1–3) (Stealth, MSS225557) and Stealth siRNA Negative Control (12935-112) were purchased from Invitrogen (Carlsbad, CA, USA). .. The sequences of short hairpin RNA (shRNAs) and siRNAs are as follows: shGEF5 no. 1, 5′-CCGGGCAACATGACAAACTTCCTATCTCGAGATAGGAAGTTTGTCATGTTGCTTTTT-3′ shGEF5 no. 2, 5′-CCGGCTCTCAAGAATCCATCTCAAACTCGAGTTTGAGATGGATTCTTGAGAGTTTTT-3′ siGEF5 no. 1, 5′-UUCAGAGGAAGGAUCUAUGAUAGGGCCCUAUCAUAGAUCCUUCCUCUGAA-3′ siGEF5 no. 2, 5′-UAAGCAGUUCACUUCCACUGCCCUGCAGGGCAGUGGAAGUGAACUGCUUA-3′ siGEF5 no. 3, 5′-UGUAUUAUUAAAUUCCUCCUGAGGGCCCUCAGGAGGAAUUUAAUAAUACA-3′.

    Article Title: Angiotensin receptor type 1 antagonists protect against neuronal injury induced by oxygen-glucose depletion
    Article Snippet: .. Stealth/siRNA duplex oligoribonucleotides (Agtr1b-RSS34217) for AT1 b RNA interference and Stealth RNAi Negative Control Duplexes (cat. no. 12935-112) were purchased from Invitrogen, Tokyo, Japan. .. The rat AT1 b RNA interference sequence was 5′-AUA CGU UUC GGU AGA UGA CGG CUG G-3′.

    Article Title: PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element–modifying factor 1
    Article Snippet: .. As control knockdown, stealth RNAi negative control #12935112 (Invitrogen) was used. .. For the knockdown of β-importin, cells were seeded as above and transfected with a mix of three siRNAs (4 nM each) from the Tri-silencer siRNA kit (Origene) using 5 µl of SilentFect reagent; the negative control was used as provided in the kit.

    Expressing:

    Article Title: ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress
    Article Snippet: .. Transfection and infection For siRNA transfection as described previously [ ], cells were reversely transfected with RNA iMAX (Life Technologies; 13778–150) and 5 nM scramble siRNA (Life Technologies, 12935–112), kinome siRNA library (2127 siRNA for 709 gene, A30079, Thermo Fisher Scientific), ATG5 (GE Healthcare Dharmacon, 9474), ATG7 (Life Technologies, s20652), BECN1 (Life Technologies, 4392420), ULK1 (Dharmacon, 8408), or ERBB2 (Life Technologies, 4390824;) for 72 h. For shRNA infection, 8XARE reporter plasmid, a packaging and VSV-G expressing envelope plasmid, were transfected into HEK293T cells using the transfection reagent, Lipofectamine 2000 (Life Technologies, 11668–027) for two days to achieve an infection. ..

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Plasmid Preparation:

    Article Title: ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress
    Article Snippet: .. Transfection and infection For siRNA transfection as described previously [ ], cells were reversely transfected with RNA iMAX (Life Technologies; 13778–150) and 5 nM scramble siRNA (Life Technologies, 12935–112), kinome siRNA library (2127 siRNA for 709 gene, A30079, Thermo Fisher Scientific), ATG5 (GE Healthcare Dharmacon, 9474), ATG7 (Life Technologies, s20652), BECN1 (Life Technologies, 4392420), ULK1 (Dharmacon, 8408), or ERBB2 (Life Technologies, 4390824;) for 72 h. For shRNA infection, 8XARE reporter plasmid, a packaging and VSV-G expressing envelope plasmid, were transfected into HEK293T cells using the transfection reagent, Lipofectamine 2000 (Life Technologies, 11668–027) for two days to achieve an infection. ..

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Flow Cytometry:

    Article Title: ATG4B promotes colorectal cancer growth independent of autophagic flux
    Article Snippet: .. The cells were seeded at 20% to 30% confluence and reversely transfected with RNAiMAX (Life Technologies, 13778-150) in the presence of 5 nM scrambled siRNA (Life Technologies, 12935-112) or siRNA against ATG4B (Life Technologies, 20218, s23245, s23246) for 72 h. For plasmid transfection, the cells were transfected with 2 μg FLAG-MTOR (26603) or HA-CCND1 (8948) expression plasmids (Addgene) or cherry-tagged ATG4B (subcloned from FLAG-ATG4B reported previously ) using 2 μl Lipofectamine 2000 (Invitrogen, 11668-027) for 24 h. The cells were harvested for either protein expression analysis by immunoblotting or cell cycle analysis by flow cytometry. .. shRNAs against ATG4B (TRCN0000073801), ATG5 (TRCN0000151963), ATG7 (TRCN0000007584), and MTOR (TRCN0000199323) were obtained from The RNAi Consortium (TRC, Taiwan). pLKO.1 Scrambled shRNA was purchased from Addgene (1864).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher silencer select pre designed sirna
    High-ranked upregulated PAGs are modifiers of cellular viability. a 78 overlapping genes were identified between human PAGs and STHdh DEGs. b Knocking-down high-ranked upregulated genes significantly alters apoptosis in STHdh Q111 cells compared to cells <t>transfected</t> with negative control <t>siRNA.</t> Calcein ( green ) stains viable cells, while propidium iodide (PI— red ) is a marker for late apoptotic cells. Scale bar =10 μm. c The number of apoptotic cells is represented as fold changes normalized to the control. Two-tailed t -test was performed to calculate statistical significance. The bar shows the mean value and the error bars indicate the standard deviation. Three independent experiments were performed, with 15 replicates each
    Silencer Select Pre Designed Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/silencer select pre designed sirna/product/Thermo Fisher
    Average 99 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    silencer select pre designed sirna - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher silencer select negative control no 1 sirna
    High-ranked upregulated PAGs are modifiers of cellular viability. a 78 overlapping genes were identified between human PAGs and STHdh DEGs. b Knocking-down high-ranked upregulated genes significantly alters apoptosis in STHdh Q111 cells compared to cells <t>transfected</t> with negative control <t>siRNA.</t> Calcein ( green ) stains viable cells, while propidium iodide (PI— red ) is a marker for late apoptotic cells. Scale bar =10 μm. c The number of apoptotic cells is represented as fold changes normalized to the control. Two-tailed t -test was performed to calculate statistical significance. The bar shows the mean value and the error bars indicate the standard deviation. Three independent experiments were performed, with 15 replicates each
    Silencer Select Negative Control No 1 Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/silencer select negative control no 1 sirna/product/Thermo Fisher
    Average 99 stars, based on 206 article reviews
    Price from $9.99 to $1999.99
    silencer select negative control no 1 sirna - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    High-ranked upregulated PAGs are modifiers of cellular viability. a 78 overlapping genes were identified between human PAGs and STHdh DEGs. b Knocking-down high-ranked upregulated genes significantly alters apoptosis in STHdh Q111 cells compared to cells transfected with negative control siRNA. Calcein ( green ) stains viable cells, while propidium iodide (PI— red ) is a marker for late apoptotic cells. Scale bar =10 μm. c The number of apoptotic cells is represented as fold changes normalized to the control. Two-tailed t -test was performed to calculate statistical significance. The bar shows the mean value and the error bars indicate the standard deviation. Three independent experiments were performed, with 15 replicates each

    Journal: Nature Communications

    Article Title: Identifying therapeutic targets by combining transcriptional data with ordinal clinical measurements

    doi: 10.1038/s41467-017-00353-6

    Figure Lengend Snippet: High-ranked upregulated PAGs are modifiers of cellular viability. a 78 overlapping genes were identified between human PAGs and STHdh DEGs. b Knocking-down high-ranked upregulated genes significantly alters apoptosis in STHdh Q111 cells compared to cells transfected with negative control siRNA. Calcein ( green ) stains viable cells, while propidium iodide (PI— red ) is a marker for late apoptotic cells. Scale bar =10 μm. c The number of apoptotic cells is represented as fold changes normalized to the control. Two-tailed t -test was performed to calculate statistical significance. The bar shows the mean value and the error bars indicate the standard deviation. Three independent experiments were performed, with 15 replicates each

    Article Snippet: After 24 h, cells were transfected with one of 50 nM Silencer Select siRNA against Sgpl1 (Thermo Fisher, 4390771: siRNA ID # s73644), 25 nM Silencer Select siRNA against Ahnak (Thermo Fisher, 4390771: siRNA ID# s83166), 50 nM Silencer Select siRNA against Tcf12 (Thermo Fisher, 4390771: siRNA ID# s74812), 50 nM Silencer Select siRNA against Tns1 (Thermo Fisher, 4390771: siRNA ID# s75344) 50 nM Silencer Select Negative Control siRNA (Thermo Fisher, 4390843) or BLOCK-iT Alexa Fluor Red Fluorescent Control (Thermo Fisher, 465318) using the Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher, 13778030).

    Techniques: Transfection, Negative Control, Marker, Two Tailed Test, Standard Deviation