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Illumina Inc 50 bp single end sequencing
Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from <t>50-bp</t> single-end DNA sequencing. P -value
50 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/50 bp single end sequencing/product/Illumina Inc
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
50 bp single end sequencing - by Bioz Stars, 2020-05
93/100 stars

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1) Product Images from "Loss of Uhrf1 in neural stem cells leads to activation of retroviral elements and delayed neurodegeneration"

Article Title: Loss of Uhrf1 in neural stem cells leads to activation of retroviral elements and delayed neurodegeneration

Journal: Genes & Development

doi: 10.1101/gad.284992.116

Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from 50-bp single-end DNA sequencing. P -value
Figure Legend Snippet: Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from 50-bp single-end DNA sequencing. P -value

Techniques Used: Activation Assay, DNA Sequencing

Related Articles

Sequencing:

Article Title: Comprehensive identification of virulence factors required for respiratory melioidosis using Tn-seq mutagenesis
Article Snippet: .. Confirmation of a 125 base pair product was conducted using 1% agarose gel electrophoresis, and libraries were sequeced using Illumina 50-bp single end sequencing at the Harvard Medical School Biopolymer Facility. ..

Article Title: Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting
Article Snippet: .. The 50-bp single-end sequencing of the constructed library was performed on the Illumina MiSeq platform at the Trinity Genome Sequencing Laboratory (TrinSeq), Trinity College Dublin. .. Raw sequencing reads were trimmed of contaminating adapter sequences using Cutadapt ( ).

Article Title: Mapping Exosome-Substrate Interactions In Vivo by UV Cross-Linking.
Article Snippet: .. The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA in eukaryotes and Archaea. .. The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA in eukaryotes and Archaea.

Article Title: Enhancer transcription reveals subtype-specific gene expression programs controlling breast cancer pathogenesis
Article Snippet: .. After library quality control assessment using a Bioanalyzer (Agilent), the samples were subjected to 50-bp single-end sequencing using an Illumina HiSeq 2000 Sequencing System. .. Additional details about the preparation of nuclei, nuclear run-ons, GRO-seq library preparation, and sequencing are provided in the .

Article Title: The ETS transcription factor ELF1 regulates a broadly antiviral program distinct from the type I interferon response
Article Snippet: .. Libraries from two biological replicates of ELF1 ChIP and two biological replicates of Input control were subject to 50-bp single end sequencing on the Illumina HiSeq 2500 platform. ..

Article Title: Loss of Uhrf1 in neural stem cells leads to activation of retroviral elements and delayed neurodegeneration
Article Snippet: .. Quality control was carried out with a Bioanalyzer (Agilent), and 50-bp single-end sequencing was performed with a HiSeq sequencer (Illumina) at LAFUGA. .. Five-hundred nanograms of MspI-digested and purified DNA from controls and Uhrf1 cKOs (E16 cortices) was used for the library preparation procedure with the NEXTflex bisulfite library preparation kit (BIOO Scientific).

Article Title: Rice In Vivo RNA Structurome Reveals RNA Secondary Structure Conservation and Divergence in Plants
Article Snippet: .. The double-stranded DNA libraries were subjected to 50-bp single-end sequencing on an Illumina HiSeq 4000 platform. .. Illumina Sequence Mapping The rice transcriptomic reference consists of nuclear, chloroplast, and mitochondrial cDNA sequences (MSU version 7.0), and rRNA and tRNA sequences (IRGSP-1.0).

Agarose Gel Electrophoresis:

Article Title: Comprehensive identification of virulence factors required for respiratory melioidosis using Tn-seq mutagenesis
Article Snippet: .. Confirmation of a 125 base pair product was conducted using 1% agarose gel electrophoresis, and libraries were sequeced using Illumina 50-bp single end sequencing at the Harvard Medical School Biopolymer Facility. ..

Chromatin Immunoprecipitation:

Article Title: The ETS transcription factor ELF1 regulates a broadly antiviral program distinct from the type I interferon response
Article Snippet: .. Libraries from two biological replicates of ELF1 ChIP and two biological replicates of Input control were subject to 50-bp single end sequencing on the Illumina HiSeq 2500 platform. ..

Construct:

Article Title: Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting
Article Snippet: .. The 50-bp single-end sequencing of the constructed library was performed on the Illumina MiSeq platform at the Trinity Genome Sequencing Laboratory (TrinSeq), Trinity College Dublin. .. Raw sequencing reads were trimmed of contaminating adapter sequences using Cutadapt ( ).

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    Illumina Inc hiseq 4000 system
    Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina <t>HiSeq</t> 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.
    Hiseq 4000 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 4000 system/product/Illumina Inc
    Average 99 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    hiseq 4000 system - by Bioz Stars, 2020-05
    99/100 stars
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    Illumina Inc 50 bp single end sequencing
    Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from <t>50-bp</t> single-end DNA sequencing. P -value
    50 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50 bp single end sequencing/product/Illumina Inc
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    50 bp single end sequencing - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Illumina Inc illumina genome analyzer
    Efficacy of individual TR enrichment steps. Total RNA and RNA samples from each of the three sequential TR enrichment steps (anti-TMG IP, exonuclease-treatment, and TERT-binding) were analyzed by <t>Illumina</t> next-generation (Next-Gen) sequencing. Read coverage
    Illumina Genome Analyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina genome analyzer/product/Illumina Inc
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    illumina genome analyzer - by Bioz Stars, 2020-05
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    Image Search Results


    Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina HiSeq 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.

    Journal: Environmental Health Perspectives

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood

    doi: 10.1289/EHP3441

    Figure Lengend Snippet: Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina HiSeq 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.

    Article Snippet: Single-end, 50 -bp reads were obtained for each library by sequencing on the HiSeq 4000 system (Illumina).

    Techniques: Sequencing, Modification, Isolation, Mouse Assay, Amplification, Quantitative RT-PCR

    Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from 50-bp single-end DNA sequencing. P -value

    Journal: Genes & Development

    Article Title: Loss of Uhrf1 in neural stem cells leads to activation of retroviral elements and delayed neurodegeneration

    doi: 10.1101/gad.284992.116

    Figure Lengend Snippet: Mechanisms of IAP activation in the Uhrf1 cKO cerebral cortices. ( A – D ) Confocal images of coronal sections of E14 ( A , B ) and E16 ( C , D ) control and cKO cortices immunostained for 5hmC. ( E ) Graph depicting repeat element classes in hmeDIP of E16 VZ tissue. The X -axis and Y -axis values are log 2 fold enrichment for controls and cKOs from 50-bp single-end DNA sequencing. P -value

    Article Snippet: Quality control was carried out with a Bioanalyzer (Agilent), and 50-bp single-end sequencing was performed with a HiSeq sequencer (Illumina) at LAFUGA.

    Techniques: Activation Assay, DNA Sequencing

    Efficacy of individual TR enrichment steps. Total RNA and RNA samples from each of the three sequential TR enrichment steps (anti-TMG IP, exonuclease-treatment, and TERT-binding) were analyzed by Illumina next-generation (Next-Gen) sequencing. Read coverage

    Journal: RNA

    Article Title: Identification of purple sea urchin telomerase RNA using a next-generation sequencing based approach

    doi: 10.1261/rna.039131.113

    Figure Lengend Snippet: Efficacy of individual TR enrichment steps. Total RNA and RNA samples from each of the three sequential TR enrichment steps (anti-TMG IP, exonuclease-treatment, and TERT-binding) were analyzed by Illumina next-generation (Next-Gen) sequencing. Read coverage

    Article Snippet: A cDNA library was constructed from extracted TERT-bound RNA and a single-end 50-bp sequencing run on an Illumina Genome Analyzer performed by Cofactor Genomics.

    Techniques: Binding Assay, Sequencing