Structured Review

Toronto Research Chemicals 5 aminoimidazole 4 carboxamide riboside aicar
Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), <t>5-aminoimidazole-4-carboxamide</t> ribonucleotide <t>(AICAR),</t> 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.
5 Aminoimidazole 4 Carboxamide Riboside Aicar, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 81/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 aminoimidazole 4 carboxamide riboside aicar/product/Toronto Research Chemicals
Average 81 stars, based on 3 article reviews
Price from $9.99 to $1999.99
5 aminoimidazole 4 carboxamide riboside aicar - by Bioz Stars, 2020-01
81/100 stars

Images

1) Product Images from "Mass spectrometric analysis of purine de novo biosynthesis intermediates"

Article Title: Mass spectrometric analysis of purine de novo biosynthesis intermediates

Journal: PLoS ONE

doi: 10.1371/journal.pone.0208947

Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.
Figure Legend Snippet: Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.

Techniques Used:

2) Product Images from "Mass spectrometric analysis of purine de novo biosynthesis intermediates"

Article Title: Mass spectrometric analysis of purine de novo biosynthesis intermediates

Journal: PLoS ONE

doi: 10.1371/journal.pone.0208947

Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.
Figure Legend Snippet: Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.

Techniques Used:

Related Articles

MTT Assay:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada). ..

Transfection:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The human hTERT-immortalized retinal pigment epithelial cell line hTERT–RPE-1 was kindly provided by Dr RM Rios (CABIMER, Sevilla, Spain) and transfected with pEGFP-LC3 plasmid ( ) to generate a stable pool of hTERT–RPE1–GFP–LC3 cells. .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Mutagenesis:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The immortalized MEFs from knock-in mice expressing an inactive TAK1 mutant (TAK1Δ) together with the appropriate wild-type MEFs were kindly provided by Dr S Akira (Osaka University, Osaka, Japan) ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: Point mutations were generated by using QuikChange mutagenesis (Stratagene). .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

Labeling:

Article Title: Mass spectrometric analysis of purine de novo biosynthesis intermediates
Article Snippet: Isotopically labeled glycine (U-13 C2 , 15 N) was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). .. AICAR, 5-aminoimidazole-4-carboxamide riboside (AICAr) and adenylosuccinic acid (SAMP) were purchased from Toronto Research Chemicals Inc. (North York, Canada).

Subcloning:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: Human and mouse LKB1 retroviral constructs were generated by PCR and subcloning into pBABE-puro. .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

Cell Culture:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: Paragraph title: Cell culture and treatments ... TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Mouse Assay:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The immortalized MEFs from knock-in mice expressing an inactive TAK1 mutant (TAK1Δ) together with the appropriate wild-type MEFs were kindly provided by Dr S Akira (Osaka University, Osaka, Japan) ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Produced:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: Recombinant human TRAIL was produced as described previously ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Knock-In:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The immortalized MEFs from knock-in mice expressing an inactive TAK1 mutant (TAK1Δ) together with the appropriate wild-type MEFs were kindly provided by Dr S Akira (Osaka University, Osaka, Japan) ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Generated:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: Point mutations were generated by using QuikChange mutagenesis (Stratagene). .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

Activity Assay:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada). .. The kinase activity of AMPK was measured by using the SAMS peptide as previously described ( ).

Construct:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: Human and mouse LKB1 retroviral constructs were generated by PCR and subcloning into pBABE-puro. .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

Expressing:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The immortalized MEFs from knock-in mice expressing an inactive TAK1 mutant (TAK1Δ) together with the appropriate wild-type MEFs were kindly provided by Dr S Akira (Osaka University, Osaka, Japan) ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Polymerase Chain Reaction:

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: STRAD was PCR-amplified from a human EST (Research Genetics, Huntsville, AL) and subcloned into pCDNA4/HisMax (Invitrogen). .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

Recombinant:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: Recombinant human TRAIL was produced as described previously ( ). .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Plasmid Preparation:

Article Title: TAK1 activates AMPK-dependent cytoprotective autophagy in TRAIL-treated epithelial cells
Article Snippet: The human hTERT-immortalized retinal pigment epithelial cell line hTERT–RPE-1 was kindly provided by Dr RM Rios (CABIMER, Sevilla, Spain) and transfected with pEGFP-LC3 plasmid ( ) to generate a stable pool of hTERT–RPE1–GFP–LC3 cells. .. TNF and IL-1β were purchased from Peprotech, rapamycin, ionomycin, pepstatin-A, E64d and 3-MA from Sigma-Aldrich (St Louis, MO, USA), 5-aminoimidazole-4-carboxamide riboside (AICAR) from Toronto Research Chemicals (Ontario, Canada), Hank's balanced salt solution starvation medium from GIBCO (CA, USA) and monoclonal antagonist antibody to TRAIL-R2 (HS201, preservative free) from Alexis Biochemicals Corp. (San Diego, CA, USA).

Article Title: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress
Article Snippet: FLAG-tagged human LKB1 was generated by subcloning the human LKB1 cDNA into an N-terminal-tagged pCDNA3 vector. .. Sorbitol, H2 O2 , and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma, and 5-aminoimidizole-4-carboxamide riboside (AICAR) was obtained from Toronto Research Chemicals (Downsview, ON, Canada).

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  • 77
    Toronto Research Chemicals aica riboside
    The combined effect of MTX and <t>AICA</t> <t>riboside</t> on inhibition of cancer cell DNA synthesis. Data are presented as mean±SD ( n =3). c P
    Aica Riboside, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aica riboside/product/Toronto Research Chemicals
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aica riboside - by Bioz Stars, 2020-01
    77/100 stars
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    81
    Toronto Research Chemicals 5 aminoimidazole 4 carboxamide riboside aicar
    Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), <t>5-aminoimidazole-4-carboxamide</t> ribonucleotide <t>(AICAR),</t> 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.
    5 Aminoimidazole 4 Carboxamide Riboside Aicar, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 aminoimidazole 4 carboxamide riboside aicar/product/Toronto Research Chemicals
    Average 81 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    5 aminoimidazole 4 carboxamide riboside aicar - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    84
    Toronto Research Chemicals 5 aminoimidazole 4 carboxamide riboside
    Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), <t>5-aminoimidazole-4-carboxamide</t> ribonucleotide <t>(AICAR),</t> 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.
    5 Aminoimidazole 4 Carboxamide Riboside, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 aminoimidazole 4 carboxamide riboside/product/Toronto Research Chemicals
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 aminoimidazole 4 carboxamide riboside - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    Image Search Results


    The combined effect of MTX and AICA riboside on inhibition of cancer cell DNA synthesis. Data are presented as mean±SD ( n =3). c P

    Journal: Acta Pharmacologica Sinica

    Article Title: Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

    doi: 10.1038/aps.2013.16

    Figure Lengend Snippet: The combined effect of MTX and AICA riboside on inhibition of cancer cell DNA synthesis. Data are presented as mean±SD ( n =3). c P

    Article Snippet: AICA riboside and AICA ribotide were obtained from Toronto Research Chemicals (Toronto, Canada).

    Techniques: Inhibition, DNA Synthesis

    Effect of MTX, AICA riboside and their combination on the growth of MCF-7 cell xenografts in BALB/c nude mice. Data are presented as mean±SD ( n =8). b P

    Journal: Acta Pharmacologica Sinica

    Article Title: Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

    doi: 10.1038/aps.2013.16

    Figure Lengend Snippet: Effect of MTX, AICA riboside and their combination on the growth of MCF-7 cell xenografts in BALB/c nude mice. Data are presented as mean±SD ( n =8). b P

    Article Snippet: AICA riboside and AICA ribotide were obtained from Toronto Research Chemicals (Toronto, Canada).

    Techniques: Mouse Assay

    Synergistic effect of MTX and AICA riboside on inhibition of MCF-7 (A) and HepG2 (B) cell proliferation in vitro . CI

    Journal: Acta Pharmacologica Sinica

    Article Title: Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

    doi: 10.1038/aps.2013.16

    Figure Lengend Snippet: Synergistic effect of MTX and AICA riboside on inhibition of MCF-7 (A) and HepG2 (B) cell proliferation in vitro . CI

    Article Snippet: AICA riboside and AICA ribotide were obtained from Toronto Research Chemicals (Toronto, Canada).

    Techniques: Inhibition, In Vitro

    Effect of MTX, AICA riboside, and their combination on AMPK phosphorylation and activity. (A) 100 μg protein was subjected to Western blot analysis with antibody against phospho-Thr172 AMPK α (pAMPK) or β-actin. Results are representative of three independent experiments. (B) Purified proteins were assayed for their ability to phosphorylate SAMS in vitro in the presence of [γ- 32 P]ATP. Values were corrected for differences in protein concentration, and expressed as mean±SD ( n =2). b P

    Journal: Acta Pharmacologica Sinica

    Article Title: Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

    doi: 10.1038/aps.2013.16

    Figure Lengend Snippet: Effect of MTX, AICA riboside, and their combination on AMPK phosphorylation and activity. (A) 100 μg protein was subjected to Western blot analysis with antibody against phospho-Thr172 AMPK α (pAMPK) or β-actin. Results are representative of three independent experiments. (B) Purified proteins were assayed for their ability to phosphorylate SAMS in vitro in the presence of [γ- 32 P]ATP. Values were corrected for differences in protein concentration, and expressed as mean±SD ( n =2). b P

    Article Snippet: AICA riboside and AICA ribotide were obtained from Toronto Research Chemicals (Toronto, Canada).

    Techniques: Activity Assay, Western Blot, Purification, In Vitro, Protein Concentration

    Combined effects of MTX and AICA riboside on inhibition of MCF-7 (A) and HepG2 (B) cell proliferation in vitro . Data are presented as mean±SD ( n =6). c P

    Journal: Acta Pharmacologica Sinica

    Article Title: Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

    doi: 10.1038/aps.2013.16

    Figure Lengend Snippet: Combined effects of MTX and AICA riboside on inhibition of MCF-7 (A) and HepG2 (B) cell proliferation in vitro . Data are presented as mean±SD ( n =6). c P

    Article Snippet: AICA riboside and AICA ribotide were obtained from Toronto Research Chemicals (Toronto, Canada).

    Techniques: Inhibition, In Vitro

    Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.

    Journal: PLoS ONE

    Article Title: Mass spectrometric analysis of purine de novo biosynthesis intermediates

    doi: 10.1371/journal.pone.0208947

    Figure Lengend Snippet: Purine de novo synthesis in humans. Abbreviations: phosphoribosylamine (PRA), glycinamideribonucleotide (GAR), N-formylglycinamide ribonucleotide (FGAR), N-formylglycinamidine ribonucleotide (FGAMR), aminoimidazole ribonucleotide (AIR), carboxyaminoimidazole ribonucleotide (CAIR), N-succinocarboxamide-5-aminoimidazole ribonucleotide (SAICAR), 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 5-formamido-4-imidazolecarboxamide ribonucleotide (FAICAR), inosine-5-monophosphate (IMP), glutamine (Gln), glutamate (Glu), pyrophosphate (PPi), adenosine-5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), glycine (Gly), phosphate (Pi), N 10 -formyl tetrahydrofolate (N 10 -formyl THF), tetrahydrofolate (THF), hydrogen carbonic acid (HCO 3- ), aspartate (Asp). For enzyme abbreviations, see the second paragraph of the Introduction.

    Article Snippet: AICAR, 5-aminoimidazole-4-carboxamide riboside (AICAr) and adenylosuccinic acid (SAMP) were purchased from Toronto Research Chemicals Inc. (North York, Canada).

    Techniques: