Journal: Journal of Pain Research
Article Title: Identification of capsazepine as a novel inhibitor of system xc− and cancer-induced bone pain
Figure Lengend Snippet: Capsazepine treatment induces the production of reactive oxygen species. Notes: Quantification of intracellular levels of reactive oxygen species (ROS) as measured by DCFDA over 24 and 48 hours of treatment with capsazepine (CPZ) and 5′-iodoresiniferatoxin (IRTX; A ). After 48 hours, 25 μM of CPZ induces a significant increase in ROS relative to dimethyl sulfoxide-treated cells ( P
Article Snippet: Cell treatmentsCapsazepine (CPZ; Cayman, Madison, WI, USA) and 5-iodoresiniferatoxin (IRTX; Alomone Labs, Jerusalem, Israel) were prepared in accordance with manufacturer’s recommendations using dimethyl sulfoxide (DMSO).