brdu injection  (Millipore)

 
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  • 96
    Name:
    5 Bromo 2 deoxyuridine 5 triphosphate sodium salt
    Description:
    5 Bromo 2 deoxyuridine 5 triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles
    Catalog Number:
    B0631
    Price:
    None
    Applications:
    5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:. in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells. to label cleaved DNA ends in apoptosis assay in avian tissue sections. for incorporation into mice prostates for immunohistochemistry studies
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    Structured Review

    Millipore brdu injection
    5 Bromo 2 deoxyuridine 5 triphosphate sodium salt
    5 Bromo 2 deoxyuridine 5 triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles
    https://www.bioz.com/result/brdu injection/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brdu injection - by Bioz Stars, 2021-05
    96/100 stars

    Images

    1) Product Images from "Nonparalytic botulinum molecules for the control of pain"

    Article Title: Nonparalytic botulinum molecules for the control of pain

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000478

    Reduced incorporation of BrdU into keratinocytes after IPLT BiTox treatment. Photomicrographs from sections of cutaneous tissue (A) 14 days after vehicle or (B) BiTox IPLT pretreatment. There was a significant reduction of BrdU positive keratinocytes 14 days or 32 days but not 3 days after BiTox pretreatment (C). Scale bar = 60 μm. Arrows point to BrdU labeled cells in the basal epidermis. Data are presented as means ± SEM, * P
    Figure Legend Snippet: Reduced incorporation of BrdU into keratinocytes after IPLT BiTox treatment. Photomicrographs from sections of cutaneous tissue (A) 14 days after vehicle or (B) BiTox IPLT pretreatment. There was a significant reduction of BrdU positive keratinocytes 14 days or 32 days but not 3 days after BiTox pretreatment (C). Scale bar = 60 μm. Arrows point to BrdU labeled cells in the basal epidermis. Data are presented as means ± SEM, * P

    Techniques Used: Labeling

    Related Articles

    Incubation:

    Article Title: Adaptor Protein LNK Is a Negative Regulator of Brain Neural Stem Cell Proliferation after Stroke
    Article Snippet: .. Free-floating sections (30 μm) were preincubated in 0.25% Triton X-100 in KPBS containing 5% donkey or goat serum for 1 h. Sections were incubated with rat anti-BrdU (1:100; Sigma), rabbit anti-p-H3, rabbit anti-IBA1 (1:1000; Wako), goat anti-Dcx (1:400; Santa Cruz Technology), rabbit anti-GFAP (1:400; Zymed), mouse anti-Nestin (1:200; Millipore Bioscience Research Reagents), mouse anti-FoxJ1 (1:1000; eBiosciences), rat anti-CD31 (1:200; BD Biosciences), or mouse anti-Sox2 (1:50; R & D Systems) primary antibodies overnight at 4°C. .. For BrdU staining, DNA was denatured in 1N HCl for 30 min at 65°C or in 2N HCl at room temperature for 2 h. Sections were washed and incubated for 2 h with Cy3-conjugated donkey anti-rat, donkey anti-rabbit (1:200; Jackson ImmunoResearch) or Alexa-Fluor 488-conjugated goat anti-mouse and donkey anti-goat secondary antibodies at room temperature (1:500; Invitrogen).

    Injection:

    Article Title: Transglutaminase inhibition stimulates hematopoiesis and reduces aggressive behavior of crayfish, Pacifastacus leniusculus
    Article Snippet: The total hemocyte and differential hemocyte (granular and semigranular) numbers after injection were subsequently counted and reported as relative hemocyte count (hemocyte number after injection divided by the hemocyte number prior to injection). .. To detect the proliferating cells, crayfish were injected with 10 μl/g fresh weight of 50 m m BrdU (Sigma-Aldrich) in CPBS for 24 h before the animals were injected with CPBS or cystamine (75 and 150 μg cystamine/g crayfish). .. At 3 h post-CPBS and -cystamine injections, the hemocytes were collected and immediately fixed with 4% paraformaldehyde in PBS for 1 h. The fixed hemocytes were treated with 2N HCl for 30 min at room temperature and washed five times, for 15 min each time, with PBST (0.5% Tween 20 in PBS buffer) before overnight incubation with mouse anti-BrdU (1:50) (BD Biosciences) in PBST at 4 °C.

    Article Title: Nonparalytic botulinum molecules for the control of pain
    Article Snippet: .. Three different groups of animals were injected with BiTox 32 days, 14 or 3 days before BrdU injection (100 mg·kg−1 in saline, i.p.; SIGMA). .. The animals were killed 24 hours after BrdU injection by deep barbiturate anaesthesia followed by transcardiac perfusion.

    Labeling:

    Article Title: Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice
    Article Snippet: Furthermore, to evaluate hippocampal proliferation, brain samples were also collected from mice that did not undergo behavioral testing ( ). .. 5-Bromo-2-deoxyuridine (BrdU) labeling To observe cellular proliferation in the hippocampus, in vivo BrdU (50 mg/kg, Sigma, Tokyo, Japan) labeling was performed. ..

    In Vivo:

    Article Title: Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice
    Article Snippet: Furthermore, to evaluate hippocampal proliferation, brain samples were also collected from mice that did not undergo behavioral testing ( ). .. 5-Bromo-2-deoxyuridine (BrdU) labeling To observe cellular proliferation in the hippocampus, in vivo BrdU (50 mg/kg, Sigma, Tokyo, Japan) labeling was performed. ..

    Immunohistochemistry:

    Article Title: The Crucial Role of Atg5 in Cortical Neurogenesis During Early Brain Development
    Article Snippet: .. The antibodies used in the immunohistochemistry and immunocytochemistry steps were mouse Sox2 (1:500, R and D Systems), mouse BrdU (1:1000, Millipore), mouse β-III-tubulin (Tuj1) (1:1000, Millipore), rabbit Tuj1 (1:1000, Sigma), mouse and rabbit LC3 (1:100, MBL), rabbit Atg5 (1:100, Abcam), rabbit β-Catenin (1:400, Cell Signaling Technology), rabbit active β-Catenin (1:400, Cell Signaling Technology), rabbit phospho-β-Catenin (Ser33/37/Thr41) (1:400, Cell Signaling Technology), and rat GFP (1:1000, MBL). .. The secondary antibodies used were conjugates of Alexa Fluor 488, Cy3, or Cy5 (1:1000, Jackson ImmunoResearch).

    Immunocytochemistry:

    Article Title: The Crucial Role of Atg5 in Cortical Neurogenesis During Early Brain Development
    Article Snippet: .. The antibodies used in the immunohistochemistry and immunocytochemistry steps were mouse Sox2 (1:500, R and D Systems), mouse BrdU (1:1000, Millipore), mouse β-III-tubulin (Tuj1) (1:1000, Millipore), rabbit Tuj1 (1:1000, Sigma), mouse and rabbit LC3 (1:100, MBL), rabbit Atg5 (1:100, Abcam), rabbit β-Catenin (1:400, Cell Signaling Technology), rabbit active β-Catenin (1:400, Cell Signaling Technology), rabbit phospho-β-Catenin (Ser33/37/Thr41) (1:400, Cell Signaling Technology), and rat GFP (1:1000, MBL). .. The secondary antibodies used were conjugates of Alexa Fluor 488, Cy3, or Cy5 (1:1000, Jackson ImmunoResearch).

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  • 97
    Millipore brdu
    The expression of Olig2 proliferation was increased in lesion regions in <t>Gpr17</t> −/− mice. ( A,B ) and ( C ) Immunostaining and quantification of for expression of OL lineage cells marker Olig2 and <t>BrdU</t> in lesion regions at 7 dpl and 14 dpl in spinal cords of 8-week-old control and Gpr17 −/− mice; n = 3 animals for each genotype. White dashed line demonstrates lesion borders. ( D ) Quantitative real-time PCR (qRT-PCR) analysis of OPCs marker PDGFRa expression in lesion regions. n = 3 animals for each genotype. Student’s t-test. Data are presented as Mean ± SEM. **P
    Brdu, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brdu/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brdu - by Bioz Stars, 2021-05
    97/100 stars
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    The expression of Olig2 proliferation was increased in lesion regions in Gpr17 −/− mice. ( A,B ) and ( C ) Immunostaining and quantification of for expression of OL lineage cells marker Olig2 and BrdU in lesion regions at 7 dpl and 14 dpl in spinal cords of 8-week-old control and Gpr17 −/− mice; n = 3 animals for each genotype. White dashed line demonstrates lesion borders. ( D ) Quantitative real-time PCR (qRT-PCR) analysis of OPCs marker PDGFRa expression in lesion regions. n = 3 animals for each genotype. Student’s t-test. Data are presented as Mean ± SEM. **P

    Journal: Scientific Reports

    Article Title: G-Protein-Coupled Receptor Gpr17 Regulates Oligodendrocyte Differentiation in Response to Lysolecithin-Induced Demyelination

    doi: 10.1038/s41598-018-22452-0

    Figure Lengend Snippet: The expression of Olig2 proliferation was increased in lesion regions in Gpr17 −/− mice. ( A,B ) and ( C ) Immunostaining and quantification of for expression of OL lineage cells marker Olig2 and BrdU in lesion regions at 7 dpl and 14 dpl in spinal cords of 8-week-old control and Gpr17 −/− mice; n = 3 animals for each genotype. White dashed line demonstrates lesion borders. ( D ) Quantitative real-time PCR (qRT-PCR) analysis of OPCs marker PDGFRa expression in lesion regions. n = 3 animals for each genotype. Student’s t-test. Data are presented as Mean ± SEM. **P

    Article Snippet: For BrdU incorporation analysis, control and Gpr17−/− littermates were injected with BrdU (Sigma-Aldrich) (100 mg per kilogram body weight) 2 hours before anaesthesia.

    Techniques: Expressing, Mouse Assay, Immunostaining, Marker, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Effects of Phb1 siRNA treatment in AML12 cell line. AML12 cells were treated with siRNA of Phb1 for 18 (expression) or 24 (growth and ChIP) hours. PHB1 mRNA level was reduced by 90% as compared to control and scrambled siRNA (SC) (A, n=6) and PHB1 protein expression decreased by 30% (B, n=3). C (n=4 to 6) shows mRNA levels in Phb1 siRNA treated AML12 cells for the genes that are up- or down-regulated most in the livers of liver-specific Phb1 ). D shows Phb1 siRNA treated AML12 cells have increased proliferation rate measured by BrDU incorporation into DNA for 4 hours (n=4). *p

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Liver-Specific Deletion of Prohibitin 1 Results in Spontaneous Liver Injury, Fibrosis, and Hepatocellular Carcinoma in Mice

    doi: 10.1002/hep.23919

    Figure Lengend Snippet: Effects of Phb1 siRNA treatment in AML12 cell line. AML12 cells were treated with siRNA of Phb1 for 18 (expression) or 24 (growth and ChIP) hours. PHB1 mRNA level was reduced by 90% as compared to control and scrambled siRNA (SC) (A, n=6) and PHB1 protein expression decreased by 30% (B, n=3). C (n=4 to 6) shows mRNA levels in Phb1 siRNA treated AML12 cells for the genes that are up- or down-regulated most in the livers of liver-specific Phb1 ). D shows Phb1 siRNA treated AML12 cells have increased proliferation rate measured by BrDU incorporation into DNA for 4 hours (n=4). *p

    Article Snippet: Cell proliferation in PHB1 silenced cells for 24h or 48h was measured by the incorporation rate of bromodeoxyuridine (BrDU) into DNA using a BrDU assay kit (CalBiochem, San Diego, CA) as described ( ) with 3,000 cells per well in 96 well plates and 4h or 1h of BrDU incorporation time for AML12 or Huh-7 cells, respectively.

    Techniques: Expressing, Chromatin Immunoprecipitation, BrdU Incorporation Assay

    Proliferation and differentiation of infiltrated bone marrow-derived cells. A , Representative BrdU staining in the lumbar spinal cord of GFP chimeric mice (14 d after injury, 11 d after BrdU injection). Note the increase of BrdU-positive cells in the lumbar spinal cord, ipsilateral to the injury (scale bar, 1 mm). B , Z-sectioned scan with confocal microscope (Zeiss LSM 510) through the extent of BrdU-positive nucleus to verify the double labeling with GFP + cells. BrdU and GFP colocalization showing cell proliferation within BM-derived microglia (yellow arrow); BrdU single labeling showing cell proliferation in resident cells (red arrowhead) (scale bar, 20 μm). C , Quantitative analysis of the number of GFP + /BrdU + cells over total BrdU + cells (14 d after injury, n = 3, 3–4 sections per animal, total of 277 BrdU + cells in DH and 201 BrdU + cells in VH were counted) indicating that, 14 d after injury, when microglial activation on the ipsilateral side reached its peak, ∼20% of proliferating cells derived from peripheral macrophages (data are presented as mean ± SEM). D , Photomicrographs showing the morphological plasticity of BM-derived cells over time. Until they were recruited into the parenchyma, they were round/oval shaped on the endothelium; shortly after their penetration (day 3 after injury), BM-derived GFP + cells displayed few short branches with a large cell body; during days 7–14, they developed into ramified cells; 30 d after injury, these infiltrated BM-derived cells differentiated into highly ramified microglia (scale bar, 100 μm).

    Journal: The Journal of Neuroscience

    Article Title: Expression of CCR2 in Both Resident and Bone Marrow-Derived Microglia Plays a Critical Role in Neuropathic Pain

    doi: 10.1523/JNEUROSCI.3016-07.2007

    Figure Lengend Snippet: Proliferation and differentiation of infiltrated bone marrow-derived cells. A , Representative BrdU staining in the lumbar spinal cord of GFP chimeric mice (14 d after injury, 11 d after BrdU injection). Note the increase of BrdU-positive cells in the lumbar spinal cord, ipsilateral to the injury (scale bar, 1 mm). B , Z-sectioned scan with confocal microscope (Zeiss LSM 510) through the extent of BrdU-positive nucleus to verify the double labeling with GFP + cells. BrdU and GFP colocalization showing cell proliferation within BM-derived microglia (yellow arrow); BrdU single labeling showing cell proliferation in resident cells (red arrowhead) (scale bar, 20 μm). C , Quantitative analysis of the number of GFP + /BrdU + cells over total BrdU + cells (14 d after injury, n = 3, 3–4 sections per animal, total of 277 BrdU + cells in DH and 201 BrdU + cells in VH were counted) indicating that, 14 d after injury, when microglial activation on the ipsilateral side reached its peak, ∼20% of proliferating cells derived from peripheral macrophages (data are presented as mean ± SEM). D , Photomicrographs showing the morphological plasticity of BM-derived cells over time. Until they were recruited into the parenchyma, they were round/oval shaped on the endothelium; shortly after their penetration (day 3 after injury), BM-derived GFP + cells displayed few short branches with a large cell body; during days 7–14, they developed into ramified cells; 30 d after injury, these infiltrated BM-derived cells differentiated into highly ramified microglia (scale bar, 100 μm).

    Article Snippet: In wild-type (C57BL/6) and GFP chimeric mice, bromodeoxyuridine (BrdU) (50 mg/kg; Sigma, St. Louis, MO) was injected intraperitoneally at day 3 after injury, and animals were killed 2 h, 4 d, 11 d, and 27 d after injection.

    Techniques: Derivative Assay, BrdU Staining, Mouse Assay, Injection, Microscopy, Labeling, Activation Assay