5 amino 1 pentanol s5  (Thermo Fisher)


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    Name:
    DMNP EDTA 1 4 5 Dimethoxy 2 Nitrophenyl 1 2 Diaminoethane N N N N Tetraacetic Acid cell impermeant
    Description:
    The caged Ca2 chelator DMNP EDTA also known as DM Nitrophen upon photolysis the Kd for Ca2 increases from 5 nM to 3 mM Thus photolysis of DMNP EDTA complexed with Ca2 results in a pulse of free Ca2
    Catalog Number:
    d6814
    Price:
    None
    Applications:
    Calcium Detection|Cell Analysis|Ionic Homeostasis & Signaling|Cell Viability, Proliferation & Function
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher 5 amino 1 pentanol s5
    Polymer structure and NP characterization. ( A ) Cationic 1-(3-aminopropyl)-4-methyl-piperazine endcapped <t>poly(1,4-butanediol-diacrylate-co-5-amino-1-pentanol)</t> (named PBAE 457) is used to electrostatically self-assemble with plasmid DNA (pDNA) to form nonviral gene delivery NPs. ( B ) Hydrodynamic diameter and zeta potential of 457/DNA NPs before and after lyophilization are similar ( n = 3 independent batches; mean ± SD).
    The caged Ca2 chelator DMNP EDTA also known as DM Nitrophen upon photolysis the Kd for Ca2 increases from 5 nM to 3 mM Thus photolysis of DMNP EDTA complexed with Ca2 results in a pulse of free Ca2
    https://www.bioz.com/result/5 amino 1 pentanol s5/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 amino 1 pentanol s5 - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Suprachoroidal gene transfer with nonviral nanoparticles"

    Article Title: Suprachoroidal gene transfer with nonviral nanoparticles

    Journal: Science Advances

    doi: 10.1126/sciadv.aba1606

    Polymer structure and NP characterization. ( A ) Cationic 1-(3-aminopropyl)-4-methyl-piperazine endcapped poly(1,4-butanediol-diacrylate-co-5-amino-1-pentanol) (named PBAE 457) is used to electrostatically self-assemble with plasmid DNA (pDNA) to form nonviral gene delivery NPs. ( B ) Hydrodynamic diameter and zeta potential of 457/DNA NPs before and after lyophilization are similar ( n = 3 independent batches; mean ± SD).
    Figure Legend Snippet: Polymer structure and NP characterization. ( A ) Cationic 1-(3-aminopropyl)-4-methyl-piperazine endcapped poly(1,4-butanediol-diacrylate-co-5-amino-1-pentanol) (named PBAE 457) is used to electrostatically self-assemble with plasmid DNA (pDNA) to form nonviral gene delivery NPs. ( B ) Hydrodynamic diameter and zeta potential of 457/DNA NPs before and after lyophilization are similar ( n = 3 independent batches; mean ± SD).

    Techniques Used: Plasmid Preparation

    Related Articles

    other:

    Article Title: Cell death assays for neurodegenerative disease drug discovery
    Article Snippet: In addition, nominally cell-impermeant dyes can leak into cells over time, causing false positive signals and potential damage when they intercalate into DNA.

    Article Title: Graded Ca2+/calmodulin-dependent coupling of voltage-gated CaV1.2 channels
    Article Snippet: On the day of experiments, the Ca2+ cage DMNP-EDTA (2 mM; Invitrogen, D6814) was added.

    Article Title: Changes in Orientation of Actin during Contraction of Muscle
    Article Snippet: 5′-iodoacetamido-tetramethyl-rhodamine-phalloidin (Rh-phalloidin), 5-dimethyoxy-2-nitrobenzyl-caged ATP (DMNPE-caged ATP), Alexa647-ATP, and 1-(4,5-dimethoxy-2-nitrophenyl)-1,2- diaminoethane- n , n , n ′, n ′-tetraacetic acid (DMNP-EDTA) were from Molecular Probes (Eugene, OR).

    Article Title: Hypocretin/Orexin Peptides Alter Spike Encoding by Serotonergic Dorsal Raphe Neurons through Two Distinct Mechanisms That Increase the Late Afterhyperpolarization
    Article Snippet: To uncage Ca2+ inside recorded neurons, 2 m m DMNP-EDTA (1-(4,5-dimethoxy-2-nitrophenyl)-1,2-diaminoethane-N,N,N′,N′-tetraacetic acid, ThermoFisher, D6814; DMNP) with 0.75 m m CaCl2 was dissolved in normal patch solution containing 100 μ m Oregon Green BAPTA 2.

    Cell Culture:

    Article Title: A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging
    Article Snippet: .. 1-(4,5-dimethoxy-2-nitrophenyl)-1,2-diaminoethane-N,N,N′,N′ -tetraacetic acid (DMNP-EDTA), octadecyl rhodamine B chloride (R18), ionomycin, calcium calibration buffer kit (C3008MP), Fluo-4 AM, and all cell culture media and supplements were obtained from Thermo Fisher. .. Tris-base was obtained from Fisher BioReagents.

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    Thermo Fisher ped6
    Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter <t>PED6</t> or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in ( a , b ) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm
    Ped6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ped6/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ped6 - by Bioz Stars, 2020-11
    99/100 stars
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    Image Search Results


    Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter PED6 or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in ( a , b ) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm

    Journal: Nature Communications

    Article Title: Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish

    doi: 10.1038/s41467-018-05105-8

    Figure Lengend Snippet: Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter PED6 or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in ( a , b ) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm

    Article Snippet: PED6 ( , Thermo Fisher) and EnzChek (E6639, Thermo Fisher) were reconstituted according to manuals.

    Techniques: In Vivo Imaging, Labeling, Activity Assay, Staining, Transmission Electron Microscopy

    Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter PED6 or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in ( a , b ) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm

    Journal: Nature Communications

    Article Title: Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish

    doi: 10.1038/s41467-018-05105-8

    Figure Lengend Snippet: Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter PED6 or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in ( a , b ) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm

    Article Snippet: PED6 (D23739, Thermo Fisher) and EnzChek (E6639, Thermo Fisher) were reconstituted according to manuals.

    Techniques: In Vivo Imaging, Labeling, Activity Assay, Staining, Transmission Electron Microscopy

    PLA 1 and PED6 substrate turnover by purified LPL and bee venom PLA 2 . Comparison demonstrates substrate preferences between purified bovine LPL and bee venom PLA 2 , using either the PLA 1 or PED6 substrate. Standard 90 min assays were run with increasing substrate concentrations. This triplicate assay represents one of two different experiments yielding identical results.

    Journal: Journal of Lipid Research

    Article Title: A novel fluorogenic substrate for the measurement of endothelial lipase activity

    doi: 10.1194/jlr.D007971

    Figure Lengend Snippet: PLA 1 and PED6 substrate turnover by purified LPL and bee venom PLA 2 . Comparison demonstrates substrate preferences between purified bovine LPL and bee venom PLA 2 , using either the PLA 1 or PED6 substrate. Standard 90 min assays were run with increasing substrate concentrations. This triplicate assay represents one of two different experiments yielding identical results.

    Article Snippet: Three microliters of PLA1 or PED6 substrate (50 μM) dissolved in DMSO was added using a Multidrop reagent dispenser (Thermo Fisher, Waltham, MA) for a final substrate concentration of 5 μM or as indicated in the figure.

    Techniques: Proximity Ligation Assay, Purification

    PED6 substrate versus PLA 1 substrate turnover by EL, HL and LPL in stably expressing HEK293 cell lines. A and B: Cells stably expressing either murine EL, hHL or hLPL were assayed using either the PED6 (A) or PLA 1 (B) substrate. For both substrates, 30 min cell-based assays were run with increasing substrate concentrations. The level of expression for the three lipases in each cell line was comparable, based on qualitative Western blotting (data not shown). These triplicate assays represent one of three different experiments yielding identical results.

    Journal: Journal of Lipid Research

    Article Title: A novel fluorogenic substrate for the measurement of endothelial lipase activity

    doi: 10.1194/jlr.D007971

    Figure Lengend Snippet: PED6 substrate versus PLA 1 substrate turnover by EL, HL and LPL in stably expressing HEK293 cell lines. A and B: Cells stably expressing either murine EL, hHL or hLPL were assayed using either the PED6 (A) or PLA 1 (B) substrate. For both substrates, 30 min cell-based assays were run with increasing substrate concentrations. The level of expression for the three lipases in each cell line was comparable, based on qualitative Western blotting (data not shown). These triplicate assays represent one of three different experiments yielding identical results.

    Article Snippet: Three microliters of PLA1 or PED6 substrate (50 μM) dissolved in DMSO was added using a Multidrop reagent dispenser (Thermo Fisher, Waltham, MA) for a final substrate concentration of 5 μM or as indicated in the figure.

    Techniques: Proximity Ligation Assay, Stable Transfection, Expressing, Western Blot

    Structures of the fluorescent substrates used. A: Chemical structure of PLA 1 -specific substrate ( N -{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza- s -indacene-3-pentanoyl}-2-hexyl- sn -glycero-3-phosphoethanolamine; mw = 849). B: Chemical structure of PED6 substrate ( N -{[6-(2,4-dinitrophenyl) amino]hexanoyl}-2-[4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza- s -indacene-3-pentanoyl}-1-hexadecanoyl- sn -glycero-3-phosphoethanolamine; mw = 1136). Positions of the sn -1 and sn -2 cleavage sites are indicated for both substrates, and the BODIPY fluor and dinotrophenyl quencher is indicated on the PLA 1 substrate.

    Journal: Journal of Lipid Research

    Article Title: A novel fluorogenic substrate for the measurement of endothelial lipase activity

    doi: 10.1194/jlr.D007971

    Figure Lengend Snippet: Structures of the fluorescent substrates used. A: Chemical structure of PLA 1 -specific substrate ( N -{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza- s -indacene-3-pentanoyl}-2-hexyl- sn -glycero-3-phosphoethanolamine; mw = 849). B: Chemical structure of PED6 substrate ( N -{[6-(2,4-dinitrophenyl) amino]hexanoyl}-2-[4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza- s -indacene-3-pentanoyl}-1-hexadecanoyl- sn -glycero-3-phosphoethanolamine; mw = 1136). Positions of the sn -1 and sn -2 cleavage sites are indicated for both substrates, and the BODIPY fluor and dinotrophenyl quencher is indicated on the PLA 1 substrate.

    Article Snippet: Three microliters of PLA1 or PED6 substrate (50 μM) dissolved in DMSO was added using a Multidrop reagent dispenser (Thermo Fisher, Waltham, MA) for a final substrate concentration of 5 μM or as indicated in the figure.

    Techniques: Proximity Ligation Assay