Article Title: ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) system
Figure Lengend Snippet: A) SDS-PAGE of RBD purification fractions. P1/P2 – insoluble fraction pellets; S1/S2 – soluble fractions; Ft – resin flow through; W – wash; E1-E7 – elution fractions. B) Disulfide bond analysis of adalimumab. C) Binding assays of SARS-CoV-2 antigens. D) Serology-based ELISA of SARS-CoV-2 antigen binding. E) SDS-PAGE of hEGF purification fractions. N – BYL non-template control; L – BYL lysate after CFPS reaction; P – insoluble fraction pellet; S – soluble fraction; Ft – resin flow through; W – wash; E1/E2 – elution fractions. F) SPR comparison of hEGF produced in either BYL or E. coli .
Article Snippet: DNA fragments were amplified with 15-20 bp of homology regions in primers using Phusion R High Fidelity DNA polymerase (New England Biolabs; NEB) and homology based assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) following manufacturer guidelines and transformed into E. coli DH5-alpha cells.
Techniques: SDS Page, Purification, Binding Assay, Enzyme-linked Immunosorbent Assay, SPR Assay, Produced