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1) Product Images from "Combination of RNAseq and SNP nanofluidic array reveals the center of genetic diversity of cacao pathogen Moniliophthora roreri in the upper Magdalena Valley of Colombia and its clonality"
Article Title: Combination of RNAseq and SNP nanofluidic array reveals the center of genetic diversity of cacao pathogen Moniliophthora roreri in the upper Magdalena Valley of Colombia and its clonality
Journal: Frontiers in Microbiology
Figure Legend Snippet: Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into DH5-alpha Escherichia coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.
Techniques Used: Sequencing, Marker, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Transformation Assay, Amplification, Generated