Journal: BMC Genomics
Article Title: Genome-wide identification of Hfq-regulated small RNAs in the fire blight pathogen Erwinia amylovora discovered small RNAs with virulence regulatory function
doi: 10.1186/1471-2164-15-414
Figure Lengend Snippet: sRNA-encoding genes in Erwinia amylovora identified by RNA-seq
Article Snippet: The intergenic regions of E. amylovora ATCC 49964 genome were analyzed by Python script ( https://github.com/alejorojas2/Common_scripts/blob/master/Upstream_Ea.py ), with the purpose of identifying sequences that meet the following criteria simultaneously: (1) containing at least 6 oligo-Us at the 3’ end; (2) containing at least 4 G + Cs in the last 6 nucleotides immediately upstream of the oligo-Us; (3) containing at least 50% G + Cs in the last 25 nucleotides immediately upstream of the oligo-Us.
Techniques: Northern Blot, Mutagenesis