mycobacterium conceptionense cip 108544 mycobacterium porcinum strain atcc 49939 afb1  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycolicibacter .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycolicibacterium .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques: Isolation, Infection

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycolicibacter .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycolicibacillus .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques:

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycobacteroides .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

doi: 10.3389/fmicb.2018.00067

Figure Lengend Snippet: Descriptions of new name combinations for species in the genus Mycolicibacterium .

Article Snippet: Mycolicibacterium porcinum comb. nov. (por.ci.num. L. neut. adj. porcinum , pertaining to swine) , Basonym: Mycobacterium porcinum Tsukamura et al. 1983 The description of this taxon is as given by Tsukamura et al. ( ). The type strain is E10241-1 = ATCC 33776 = CCUG 37674 = CIP 105392 = DSM 44242 = JCM 6378. .

Techniques: Isolation, Infection

Journal: Thyroid

Article Title: Regulation of Dual Oxidase Expression and H 2 O 2 Production by Thyroglobulin

doi: 10.1089/thy.2012.0003

Figure Lengend Snippet: Tg suppressed the mRNA expression of Duox2 and Duoxa2, but not Duox1 or Duoxa1. FRTL-5 cells grown in the presence (■) or absence (□) of TSH; insulin and 5% serum were treated with Tg for 6 and 24 hours. Total RNA was isolated at the indicated time after Tg stimulation, and DNA microarray analysis was performed as described in the Materials and Methods section. The normalized signal intensities reflecting the mRNA levels of Duox1 (A), Duoxa1 (B), Duox2 (C), and Duoxa2 (D) at 6 and 24 hours after Tg stimulation were compared.

Article Snippet: The membranes were washed with PBS with 0.1% Tween 20, and then incubated with either goat polyclonal anti-β-actin (Santa Cruz Biotechnology; dilution 1:1000) or goat polyclonal anti-DUOX2 antibody (sc-49939) (Santa Cruz; dilution 1:4000) for 12 hours at 4°C.

Techniques: Expressing, Isolation, Microarray

Journal: Thyroid

Article Title: Regulation of Dual Oxidase Expression and H 2 O 2 Production by Thyroglobulin

doi: 10.1089/thy.2012.0003

Figure Lengend Snippet: Tg modulated Duox2 and Duoxa2 mRNA levels in a concentration-dependent manner. FRTL-5 cells were treated with 1–30 mg/mL of Tg for 24 hours, and a quantitative real-time PCR analysis of Duox1 (A), Duoxa1 (B), Duox2 (C), and Duoxa2 (D) was performed. Data represent means±SD of three samples. Results are normalized against β-actin and displayed relative to the original levels. *p<0.05, **p<0.01 vs. no treatment with Tg.

Article Snippet: The membranes were washed with PBS with 0.1% Tween 20, and then incubated with either goat polyclonal anti-β-actin (Santa Cruz Biotechnology; dilution 1:1000) or goat polyclonal anti-DUOX2 antibody (sc-49939) (Santa Cruz; dilution 1:4000) for 12 hours at 4°C.

Techniques: Concentration Assay, Real-time Polymerase Chain Reaction

Journal: Thyroid

Article Title: Regulation of Dual Oxidase Expression and H 2 O 2 Production by Thyroglobulin

doi: 10.1089/thy.2012.0003

Figure Lengend Snippet: Tg suppressed Duox2 (A) and Duoxa2 (B) mRNA levels as a function of time. FRTL-5 cells were treated with 10 mg/mL of Tg, and total RNA was isolated 1–72 hours after stimulation. Quantitative real-time PCR analysis was performed, and the results are normalized against β-actin and displayed relative to the original levels. Data represent means±SD of three samples. *p<0.01, **p<0.001 vs. 0 time point.

Article Snippet: The membranes were washed with PBS with 0.1% Tween 20, and then incubated with either goat polyclonal anti-β-actin (Santa Cruz Biotechnology; dilution 1:1000) or goat polyclonal anti-DUOX2 antibody (sc-49939) (Santa Cruz; dilution 1:4000) for 12 hours at 4°C.

Techniques: Isolation, Real-time Polymerase Chain Reaction

Journal: Thyroid

Article Title: Regulation of Dual Oxidase Expression and H 2 O 2 Production by Thyroglobulin

doi: 10.1089/thy.2012.0003

Figure Lengend Snippet: Tg suppressed DUOX2 promoter activity. Luciferase reporter gene constructs containing the full-length (5′-932) or the truncated (5′-626) DUOX2 promoter fragment were transiently transfected in FRTL-5 cells. The cells were then stimulated with 5–30 mg/mL of Tg as indicated, and luciferase activities were measured 48h after Tg stimulation. Data were normalized by corresponding protein concentrations. Luciferase activity is expressed as means±SD of RLU from four samples. *p<0.05, **p<0.01, ***p<0.001 vs. no treatment with Tg. RLU, relative light units.

Article Snippet: The membranes were washed with PBS with 0.1% Tween 20, and then incubated with either goat polyclonal anti-β-actin (Santa Cruz Biotechnology; dilution 1:1000) or goat polyclonal anti-DUOX2 antibody (sc-49939) (Santa Cruz; dilution 1:4000) for 12 hours at 4°C.

Techniques: Activity Assay, Luciferase, Construct, Transfection

Journal: Thyroid

Article Title: Regulation of Dual Oxidase Expression and H 2 O 2 Production by Thyroglobulin

doi: 10.1089/thy.2012.0003

Figure Lengend Snippet: Tg suppressed DUOX2 protein levels in FRTL-5 cells. FRTL-5 cells cultured in the presence of TSH, insulin, and 5% serum were treated with 10 mg/mL of Tg. The effect of Tg on the reduction of DUOX2 protein levels was evaluated by Western blot analysis (A) using the anti-DUOX2 antibody from 3 to 48 hours after Tg stimulation. A densitometric image analysis of the blot (B) showed a gradual decrease in the DUOX2 protein levels after Tg treatment.

Article Snippet: The membranes were washed with PBS with 0.1% Tween 20, and then incubated with either goat polyclonal anti-β-actin (Santa Cruz Biotechnology; dilution 1:1000) or goat polyclonal anti-DUOX2 antibody (sc-49939) (Santa Cruz; dilution 1:4000) for 12 hours at 4°C.

Techniques: Cell Culture, Western Blot