Journal:
Article Title: Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism of gap Gene
doi: 10.1128/JCM.39.10.3693-3695.2001
Figure Lengend Snippet: (A) Agarose gel electrophoresis of PCR amplification products from Staphylococcus reference strains (Table (Table1)1) using primers GF-1 and GR-2. Lanes: 1, Staphylococcus arlettae; 2, Staphylococcus capitis subsp. capitis; 3, Staphylococcus capitis subsp. urealyticus; 4, Staphylococcus chromogenes; 5, Staphylococcus cohnii subsp. cohnii; 6, Staphylococcus cohnii subsp. urealyticum; 7, Staphylococcus delphini; 8, Staphylococcus equorum; 9, Staphylococcus gallinarum; 10, Staphylococcus kloosii; 11, Staphylococcus lentus; 12, Staphylococcus muscae; 13, Staphylococcus piscifermentans; 14, Staphylococcus pulvereri; 15, Staphylococcus caseolyticus (Macrococcus caseolyticus); M, DNA molecular mass markers (φX174 phage, HaeIII digested). (B) Agarose gel electrophoresis of the fragments produced by AluI digestion of the 933-bp PCR amplification products from Staphylococcus species. Lanes are the same as in panel A. M, DNA molecular mass markers, 50-bp ladder; bottom band, 50 bp.
Article Snippet: Strains were grown on Luria agar or Luria broth supplemented with glucose or yeast extract and incubated at 37°C except for S. equorum which was incubated at 30°C. table ft1 table-wrap mode="anchored" t5 caption a7 Strain gap PCR amplification Source a Staphylococcus arlettae + DMSZ 20672 (ATCC 43957) Staphylococcus capitis subsp. capitis + DMSZ 20326 (ATCC 27840) Staphylococcus capitis subsp. urealyticus + DMSZ 6717 (ATCC 49326) Staphylococcus chromogenes + DMSZ 20454 (ATCC 43764) Staphylococcus cohnii subsp. cohnii + DMSZ 20260 (ATCC 29974) Staphylococcus cohnii subsp. urealyticum + DMSZ 6718 (ATCC 49930) Staphylococcus delphini + DMSZ 20771 (ATCC 49171) Staphylococcus equorum + DMSZ 20674 (ATCC 43958) Staphylococcus gallinarum + DMSZ 20610 (ATCC 35539) Staphylococcus kloosii + DMSZ 20676 (ATCC 43959) Staphylococcus lentus + DMSZ 20352 (ATCC 29070) Staphylococcus muscae + DMSZ 7068 (ATCC 49910) Staphylococcus piscifermentans + DMSZ 7373 (ATCC 51136) Staphylococcus pulvereri + DMSZ 9930 (ATCC 51698) Staphylococcus caseolyticus − DMSZ 20597 (ATCC 13548) Open in a separate window a DMSZ, Deutsche Sammulung von Mikroorganismen und Zellkulturen GmbH; ATCC, American Type Culture Collection.
Techniques: Agarose Gel Electrophoresis, Amplification, Produced