Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Coaggregation [%] of LAB strains with honeybee pathogens after 24 h incubation. The presented results are the mean from three repeats ± standard error of the mean (S.E.M.).

Article Snippet: The different results obtained for the co-aggregation of LAB with P. larvae ATCC 25367 and ATCC 49843 suggest that this is a unique feature in relation to pathogen strains belonging to the same species.

Techniques: Incubation

Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Eradication (+) and stimulation (−) of pathogen biofilms [%] by cell-free supernatants (CFSs) from lactic acid bacteria (LAB) after 24 h incubation. Letters A, B and C indicate the pairs of CFS for which the eradication properties of pathogen biofilms were significantly different. The presented results are the mean from eight individual wells ± standard error of the mean (S.E.M.).

Article Snippet: The different results obtained for the co-aggregation of LAB with P. larvae ATCC 25367 and ATCC 49843 suggest that this is a unique feature in relation to pathogen strains belonging to the same species.

Techniques: Incubation, Significance Assay

Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Selected microphotographs showing the effect of cell-free supernatants (CFSs) from L. plantarum 21/1 on biofilm eradication of: 1A P. larvae ATCC 25367; 2A P. larvae ATCC 49843; 3A L. sphaericus DSM 1866; 4A M. plutonius DSM 29964. 1 – 4B : pathogen cells without CFSs (negative controls) (objective 20×). Observed under a phase-contrast microscope (Nikon Eclipse Ci H600L, Tokyo, Japan) attached to a digital camera (NikonDigital Sight DS-U3, Tokyo, Japan) and imaging software (NIS-elements BR 3.0, Nikon, Tokyo, Japan).

Article Snippet: The different results obtained for the co-aggregation of LAB with P. larvae ATCC 25367 and ATCC 49843 suggest that this is a unique feature in relation to pathogen strains belonging to the same species.

Techniques: Microscopy, Imaging, Software

Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Coaggregation [%] of LAB strains with honeybee pathogens after 24 h incubation. The presented results are the mean from three repeats ± standard error of the mean (S.E.M.).

Article Snippet: P. larvae ATCC 49843 , 87.16 ± 1.27 * , 42.96 ± 1.38 * , 47.04 ± 2.29 , 48.33 ± 2.56 , p = 0.043.

Techniques: Incubation

Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Eradication (+) and stimulation (−) of pathogen biofilms [%] by cell-free supernatants (CFSs) from lactic acid bacteria (LAB) after 24 h incubation. Letters A, B and C indicate the pairs of CFS for which the eradication properties of pathogen biofilms were significantly different. The presented results are the mean from eight individual wells ± standard error of the mean (S.E.M.).

Article Snippet: P. larvae ATCC 49843 , 87.16 ± 1.27 * , 42.96 ± 1.38 * , 47.04 ± 2.29 , 48.33 ± 2.56 , p = 0.043.

Techniques: Incubation, Significance Assay

Journal: Molecules

Article Title: Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( Apis mellifera L.) Pathogens

doi: 10.3390/molecules27248945

Figure Lengend Snippet: Selected microphotographs showing the effect of cell-free supernatants (CFSs) from L. plantarum 21/1 on biofilm eradication of: 1A P. larvae ATCC 25367; 2A P. larvae ATCC 49843; 3A L. sphaericus DSM 1866; 4A M. plutonius DSM 29964. 1 – 4B : pathogen cells without CFSs (negative controls) (objective 20×). Observed under a phase-contrast microscope (Nikon Eclipse Ci H600L, Tokyo, Japan) attached to a digital camera (NikonDigital Sight DS-U3, Tokyo, Japan) and imaging software (NIS-elements BR 3.0, Nikon, Tokyo, Japan).

Article Snippet: P. larvae ATCC 49843 , 87.16 ± 1.27 * , 42.96 ± 1.38 * , 47.04 ± 2.29 , 48.33 ± 2.56 , p = 0.043.

Techniques: Microscopy, Imaging, Software