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mesoplasma coleopterae  (ATCC)


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    ATCC mesoplasma coleopterae
    Forty years of research on <t>Mesoplasma</t> florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.
    Mesoplasma Coleopterae, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mesoplasma florum : a near-minimal model organism for systems and synthetic biology"

    Article Title: Mesoplasma florum : a near-minimal model organism for systems and synthetic biology

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2024.1346707

    Forty years of research on Mesoplasma florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.
    Figure Legend Snippet: Forty years of research on Mesoplasma florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.

    Techniques Used: Electron Microscopy, Construct

    List of Mesoplasma and Entomoplasma strains with genome assemblies deposited on the RefSeq database.
    Figure Legend Snippet: List of Mesoplasma and Entomoplasma strains with genome assemblies deposited on the RefSeq database.

    Techniques Used: Isolation

    Genome engineering tools and projects to transform Mesoplasma florum into an optimized cell chassis for systems and synthetic biology. (A) Whole genome cloning and transplantation procedure. Bacterial genomes containing a yeast vector are first transformed in yeast to allow genetic modifications using the available molecular tools. Modified genomes are then carefully isolated and transplanted into a compatible recipient bacterium. Recipient cells adopt the phenotype conferred by the transplanted genome (see ; ). (B) Serine integrase mediated genome engineering methodology. DNA fragments such as genes of interest (GOI) can be inserted or exchanged with the genome by the expression of a serine integrase. Serine integrases such as Bxb1 or PhiC31 catalyze the recombination between specific DNA sequences, namely, the attP and attB sites, resulting in attL and attR sites ( attL sites not shown, see for more details). (C) Integration of multiple data types by computer-aided design (CAD) tools to guide the design and optimize the synthesis, amplification, and assembly of large DNA fragments according to user-defined constraints. In silico models such as genome-scale models (GEMs) can be used to predict the fitness of designed genomes. (D) to (G) Example of synthetic genome projects. (D) Genome reduction, in which non-essential genes are removed from the genome, resulting in a reduced cell complexity and simplified metabolism. (E) Gene recoding at the genome scale. Within a given open reading frame, codons can be exchanged for synonymous ones, modifying the DNA sequence but not the corresponding amino acid sequence. (F) Streamlined genome using a swapped amino acid genetic code. By removing non-essential genes and recoding all remaining genes, specific codons can be removed from the genome, allowing anticodon swap between given tRNAs and creating an artificial genetic code. (G) Streamlined genome with gene of similar function regrouped in modules. Regrouping genes with related functions into modules can facilitate genome engineering efforts, and can be used to test specific hypotheses about gene regulation and genome organization.
    Figure Legend Snippet: Genome engineering tools and projects to transform Mesoplasma florum into an optimized cell chassis for systems and synthetic biology. (A) Whole genome cloning and transplantation procedure. Bacterial genomes containing a yeast vector are first transformed in yeast to allow genetic modifications using the available molecular tools. Modified genomes are then carefully isolated and transplanted into a compatible recipient bacterium. Recipient cells adopt the phenotype conferred by the transplanted genome (see ; ). (B) Serine integrase mediated genome engineering methodology. DNA fragments such as genes of interest (GOI) can be inserted or exchanged with the genome by the expression of a serine integrase. Serine integrases such as Bxb1 or PhiC31 catalyze the recombination between specific DNA sequences, namely, the attP and attB sites, resulting in attL and attR sites ( attL sites not shown, see for more details). (C) Integration of multiple data types by computer-aided design (CAD) tools to guide the design and optimize the synthesis, amplification, and assembly of large DNA fragments according to user-defined constraints. In silico models such as genome-scale models (GEMs) can be used to predict the fitness of designed genomes. (D) to (G) Example of synthetic genome projects. (D) Genome reduction, in which non-essential genes are removed from the genome, resulting in a reduced cell complexity and simplified metabolism. (E) Gene recoding at the genome scale. Within a given open reading frame, codons can be exchanged for synonymous ones, modifying the DNA sequence but not the corresponding amino acid sequence. (F) Streamlined genome using a swapped amino acid genetic code. By removing non-essential genes and recoding all remaining genes, specific codons can be removed from the genome, allowing anticodon swap between given tRNAs and creating an artificial genetic code. (G) Streamlined genome with gene of similar function regrouped in modules. Regrouping genes with related functions into modules can facilitate genome engineering efforts, and can be used to test specific hypotheses about gene regulation and genome organization.

    Techniques Used: Clone Assay, Transplantation Assay, Plasmid Preparation, Transformation Assay, Modification, Isolation, Expressing, Amplification, In Silico, Sequencing



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    Forty years of research on <t>Mesoplasma</t> florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.
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    Forty years of research on <t>Mesoplasma</t> florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.
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    Forty years of research on <t>Mesoplasma</t> florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.
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    Image Search Results


    Forty years of research on Mesoplasma florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.

    Journal: Frontiers in Genetics

    Article Title: Mesoplasma florum : a near-minimal model organism for systems and synthetic biology

    doi: 10.3389/fgene.2024.1346707

    Figure Lengend Snippet: Forty years of research on Mesoplasma florum . (A) Important milestones in M. florum research timeline. Representative picture of an M. florum L1 colony displaying the typical “fried-egg” morphology (adapted from , Vol. 44, No. 17, pp. 8501–8511, by permission of Oxford University Press; scale bar: 100 µm) as well as M. florum L1 cells observed by scanning electron microscopy are also depicted. (B) and (C) Maximum-likelihood phylogenetic trees of the Mollicutes (B) and the Mesoplasma/Entomoplasma genera (C) inferred using concatenated alignments of 109 and 229 conserved proteins, respectively. Trees were constructed using RAxML with 150 bootstrap replicates as determined using the autoFC bootstopping criterion. Bootstrap replicate values are of 100 unless specified otherwise. Bacillus subtilis and S. citri were used as outgroups. See for additional information on strains and genomes included in the Mesoplasma/Entomoplasma phylogenetic tree.

    Article Snippet: Mesoplasma coleopterae , - , BARC 779 (ATCC 49583) , , Chauliognathus sp , Gut of adult soldier beetles , GCF_002804245.1 , Academia Sinica , 04/12/2017 , Complete , 800,407.

    Techniques: Electron Microscopy, Construct

    List of Mesoplasma and Entomoplasma strains with genome assemblies deposited on the RefSeq database.

    Journal: Frontiers in Genetics

    Article Title: Mesoplasma florum : a near-minimal model organism for systems and synthetic biology

    doi: 10.3389/fgene.2024.1346707

    Figure Lengend Snippet: List of Mesoplasma and Entomoplasma strains with genome assemblies deposited on the RefSeq database.

    Article Snippet: Mesoplasma coleopterae , - , BARC 779 (ATCC 49583) , , Chauliognathus sp , Gut of adult soldier beetles , GCF_002804245.1 , Academia Sinica , 04/12/2017 , Complete , 800,407.

    Techniques: Isolation

    Genome engineering tools and projects to transform Mesoplasma florum into an optimized cell chassis for systems and synthetic biology. (A) Whole genome cloning and transplantation procedure. Bacterial genomes containing a yeast vector are first transformed in yeast to allow genetic modifications using the available molecular tools. Modified genomes are then carefully isolated and transplanted into a compatible recipient bacterium. Recipient cells adopt the phenotype conferred by the transplanted genome (see ; ). (B) Serine integrase mediated genome engineering methodology. DNA fragments such as genes of interest (GOI) can be inserted or exchanged with the genome by the expression of a serine integrase. Serine integrases such as Bxb1 or PhiC31 catalyze the recombination between specific DNA sequences, namely, the attP and attB sites, resulting in attL and attR sites ( attL sites not shown, see for more details). (C) Integration of multiple data types by computer-aided design (CAD) tools to guide the design and optimize the synthesis, amplification, and assembly of large DNA fragments according to user-defined constraints. In silico models such as genome-scale models (GEMs) can be used to predict the fitness of designed genomes. (D) to (G) Example of synthetic genome projects. (D) Genome reduction, in which non-essential genes are removed from the genome, resulting in a reduced cell complexity and simplified metabolism. (E) Gene recoding at the genome scale. Within a given open reading frame, codons can be exchanged for synonymous ones, modifying the DNA sequence but not the corresponding amino acid sequence. (F) Streamlined genome using a swapped amino acid genetic code. By removing non-essential genes and recoding all remaining genes, specific codons can be removed from the genome, allowing anticodon swap between given tRNAs and creating an artificial genetic code. (G) Streamlined genome with gene of similar function regrouped in modules. Regrouping genes with related functions into modules can facilitate genome engineering efforts, and can be used to test specific hypotheses about gene regulation and genome organization.

    Journal: Frontiers in Genetics

    Article Title: Mesoplasma florum : a near-minimal model organism for systems and synthetic biology

    doi: 10.3389/fgene.2024.1346707

    Figure Lengend Snippet: Genome engineering tools and projects to transform Mesoplasma florum into an optimized cell chassis for systems and synthetic biology. (A) Whole genome cloning and transplantation procedure. Bacterial genomes containing a yeast vector are first transformed in yeast to allow genetic modifications using the available molecular tools. Modified genomes are then carefully isolated and transplanted into a compatible recipient bacterium. Recipient cells adopt the phenotype conferred by the transplanted genome (see ; ). (B) Serine integrase mediated genome engineering methodology. DNA fragments such as genes of interest (GOI) can be inserted or exchanged with the genome by the expression of a serine integrase. Serine integrases such as Bxb1 or PhiC31 catalyze the recombination between specific DNA sequences, namely, the attP and attB sites, resulting in attL and attR sites ( attL sites not shown, see for more details). (C) Integration of multiple data types by computer-aided design (CAD) tools to guide the design and optimize the synthesis, amplification, and assembly of large DNA fragments according to user-defined constraints. In silico models such as genome-scale models (GEMs) can be used to predict the fitness of designed genomes. (D) to (G) Example of synthetic genome projects. (D) Genome reduction, in which non-essential genes are removed from the genome, resulting in a reduced cell complexity and simplified metabolism. (E) Gene recoding at the genome scale. Within a given open reading frame, codons can be exchanged for synonymous ones, modifying the DNA sequence but not the corresponding amino acid sequence. (F) Streamlined genome using a swapped amino acid genetic code. By removing non-essential genes and recoding all remaining genes, specific codons can be removed from the genome, allowing anticodon swap between given tRNAs and creating an artificial genetic code. (G) Streamlined genome with gene of similar function regrouped in modules. Regrouping genes with related functions into modules can facilitate genome engineering efforts, and can be used to test specific hypotheses about gene regulation and genome organization.

    Article Snippet: Mesoplasma coleopterae , - , BARC 779 (ATCC 49583) , , Chauliognathus sp , Gut of adult soldier beetles , GCF_002804245.1 , Academia Sinica , 04/12/2017 , Complete , 800,407.

    Techniques: Clone Assay, Transplantation Assay, Plasmid Preparation, Transformation Assay, Modification, Isolation, Expressing, Amplification, In Silico, Sequencing