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Phase 1 of the study consisted of two steps: (1) comparing RNA quality when using three different RNA extraction kits for stool collected in RNAlater and (2) comparing RNA quality for RNA extracted from stool collected in four different preservation conditions, including no preservative (NP), with RNA extracted using the BIOMICs RNA extraction kit. For each step, RNA quality number was determined using a fragment analyzer. Phase 2 of the study entailed collecting paired aliquots from the same stool sample into RNAlater versus Shield for four infants. RNA was extracted using the BIOMICs RNA extraction kit. MiRNAs were then quantified for each sample using miRNA-seq. (Figure created in Biorender).

Journal: bioRxiv

Article Title: Optimizing Protocols for MicroRNA Profiling of Infant and Toddler Stool

doi: 10.1101/2025.04.01.646630

Figure Lengend Snippet: Phase 1 of the study consisted of two steps: (1) comparing RNA quality when using three different RNA extraction kits for stool collected in RNAlater and (2) comparing RNA quality for RNA extracted from stool collected in four different preservation conditions, including no preservative (NP), with RNA extracted using the BIOMICs RNA extraction kit. For each step, RNA quality number was determined using a fragment analyzer. Phase 2 of the study entailed collecting paired aliquots from the same stool sample into RNAlater versus Shield for four infants. RNA was extracted using the BIOMICs RNA extraction kit. MiRNAs were then quantified for each sample using miRNA-seq. (Figure created in Biorender).

Article Snippet: Three different stool RNA extraction kits were tested for their ability to isolate high quality RNA from infant/toddler stool: Stool Total RNA Purification Kit (Norgen Biotek 49500), (2) Quick-RNA Fecal/Soil Microbe Microprep Kit (Zymo R2040) (Quick-RNA) and (3) Zymo BIOMICs RNA Miniprep (Zymo R2001) (BIOMICs).

Techniques: RNA Extraction, Preserving

Overview of RNA extraction kits and protocols.

Journal: Scientific Reports

Article Title: Selection of optimal extraction and RT-PCR protocols for stool RNA detection of colorectal cancer associated immune genes

doi: 10.1038/s41598-024-78680-0

Figure Lengend Snippet: Overview of RNA extraction kits and protocols.

Article Snippet: The Nanodrop method showed that the Stool Total RNA Purification kit from Norgen yielded higher RNA concentrations in comparison to the Qiagen and BioMérieux methods, with statistically significant differences (p value 0.006 and p value < 0.001, respectively) (Fig. B; Table ).

Techniques: RNA Extraction, Lysis, Purification, Extraction

Overview of RNA extraction kits and protocols.

Journal: Scientific Reports

Article Title: Selection of optimal extraction and RT-PCR protocols for stool RNA detection of colorectal cancer associated immune genes

doi: 10.1038/s41598-024-78680-0

Figure Lengend Snippet: Overview of RNA extraction kits and protocols.

Article Snippet: Stool total RNA purification (Norgen) , Bead tube (Norgen Biotek) with silica beads and 1 mL of non-ionic detergent-based lysis buffer C , Spin-column-based solid-phase extraction (resin separation matrix) , miRNA, mRNA rRNA , Approx. 180 min.

Techniques: RNA Extraction, Lysis, Purification, Extraction