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Peroxy Orange 1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g vaginalis  (DSMZ)
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G Vaginalis, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fannyhessea vaginae  (DSMZ)
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Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), <t>Fannyhessea</t> vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.
Fannyhessea Vaginae, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genomic dna of bifidobacterium vaginale dsm 4944  (DSMZ)
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DSMZ genomic dna of bifidobacterium vaginale dsm 4944
Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), <t>Fannyhessea</t> vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.
Genomic Dna Of Bifidobacterium Vaginale Dsm 4944, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1/4” pressure-field microphone (ccld pressure-field, type 4944-a, bruel & kjaer)  (Bruel Kjaer GmbH)
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Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), <t>Fannyhessea</t> vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.
1/4” Pressure Field Microphone (Ccld Pressure Field, Type 4944 A, Bruel & Kjaer), supplied by Bruel Kjaer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxy orange 1 tocris bioscience  (Tocris)
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Tocris peroxy orange 1 tocris bioscience
Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), <t>Fannyhessea</t> vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.
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gardnerella vaginalis  (DSMZ)
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DSMZ gardnerella vaginalis
Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], <t>Gardnerella</t> <t>vaginalis</t> [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.
Gardnerella Vaginalis, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lactobacillus iners  (DSMZ)
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DSMZ lactobacillus iners
Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( <t>Lactobacillus</t> crispatus [LC], Lactobacillus <t>iners</t> [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.
Lactobacillus Iners, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: bioRxiv

Article Title: Sialidase variability in Gardnerella : genetics, taxonomy, function, and clinical presentation

doi: 10.64898/2026.01.10.698787

Figure Lengend Snippet:

Article Snippet: The type strain of G. vaginalis (DSM 4944) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) and -80 °C freezer stocks were prepared as described above.

Techniques:

Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), Fannyhessea vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.

Journal: European Journal of Immunology

Article Title: Vaginal Prevotella timonensis Bacteria Enhance HIV‐1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets

doi: 10.1002/eji.202451192

Figure Lengend Snippet: Prevotella timonensis increases HIV‐1 uptake in moDCs. MoDCs were stimulated with UV‐inactivated bacteria for 16 h (731 ng) and subsequently exposed to HIV‐1 (SF162; 7.3 ng) for 24 h unless otherwise specified. HIV‐1 uptake was quantified by p24 ELISA following cell lysis. (A) MoDCs were exposed to Lactobacillus crispatus (LC), Gardnerella vaginalis (GV), Fannyhessea vaginae (FV), Megasphaera elsdenii (ME), Prevotella timonensis (PT), Bacteroides fragilis (BF), Prevotella bivia (PB), Prevotella copri (PC), Prevotella intermedia (PI), or LPS derived from Salmonella typhosa (10 ng/mL). (B) MoDCs were stimulated with increasing amounts of P. timonensis (14.6, 146, 731 ng). (C) MoDCs were exposed for 6 h to 20 ng of lab‐adapted HIV‐1 strains NL4.3 (HEK293T‐produced) and SF162 (PBMC‐produced), and Transmitted Founder (T/F) variants CH058 and THRO. (D) MoDCs were pre‐treated with a DC‐SIGN blocking antibody (AZN‐D1, 20 µg/mL), CLR inhibitor Mannan (100 µg/mL), a CCR5 antagonist (Maraviroc, 30 µM), or a CD4 blocking antibody (RPA‐T4, 20 µg/mL) for 45 min prior to 6 h HIV‐1 exposure. Experiments were performed for four (C, D) to six (A, B) different donors measured in triplicate. Symbols represent independent donors, bars represent mean + SD. Statistical analysis was performed using a two‐way ANOVA with Tukey's multiple comparisons test. **** p < 0.0001, * p < 0.05.

Article Snippet: Bacteroides fragilis (ATCC‐25285), Fannyhessea vaginae (DSMZ‐15829), Gardnerella vaginalis (DSMZ‐4944), Lactobacillus crispatus (DSMZ‐20584), Megasphaera elsdenii (DSMZ‐20460), P. bivia (DSMZ‐20514), P. copri (DSMZ‐18205), P. intermedia (DSMZ‐20706), and P. timonensis CRIS 5C‐B1 (alternative name Hoylesella timonensis [ ], BEI Resources, HM‐136) bacteria were cultured as recommended by DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Lysis, Derivative Assay, Produced, Blocking Assay

Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.

Journal: The Journal of Infectious Diseases

Article Title: Prevotella timonensis Bacteria Associated With Vaginal Dysbiosis Enhance Human Immunodeficiency Virus Type 1 Susceptibility Of Vaginal CD4 + T Cells

doi: 10.1093/infdis/jiae166

Figure Lengend Snippet: Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.

Article Snippet: Lactobacillus crispatus (German Collection of Microorganisms and Cell Cultures GmbH [DSMZ]-20584), Lactobacillus iners (DSMZ-13335), Gardnerella vaginalis (DSMZ-4944), Fannyhessea vaginae (DMSZ-15829), Megasphaera elsdenii (DSMZ-20460), Bacteroides fragilis (ATCC-25285), Bacteroides thetaiotaomicron (DSMZ-2079), Escherichia coli (NC0749147), Prevotella amnii (DSMZ-23384), Prevotella bivia (DSMZ-20514), Prevotella copri (or Segatella copri ; DSMZ-18205), Prevotella intermedia (DSMZ-20706), and Prevotella timonensis (or Hoylesella timonensis ; DSMZ-22865) were cultured as recommended by DSMZ.

Techniques: Virus, Isolation, Bacteria, Infection, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Lysis, Activity Assay, Standard Deviation

Antiretroviral drugs used in pre-exposure prophylaxis (PrEP) and combination antiretroviral therapy (cART) block Prevotella timonensis –enhanced human immunodeficiency virus type 1 (HIV-1) infection in vaginal CD4 + T cells. A–C , CD4 + T cells isolated from phytohemagglutinin (PHA)–stimulated peripheral blood mononuclear cells (PBMCs) ( A and B ) or vaginal epithelium explants ( C ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d. HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of total cells (CD4 + T cells isolated from PHA-stimulated PBMCs ( A and B ) or CD3 + cells of emigrated fraction (explant model, C ). HIV-1 infection in the presence or absence of the PrEP drug tenofovir (TFV, reverse-transcriptase inhibitor, 50 µM, A , n = 4) or replication inhibitors used in cART ( B , n = 3; C , n = 5); maraviroc (Mar, CCR5 blockage, 30 µM); reverse transcriptase inhibitors zidovudine (ZDV, 20 µM) and lamivudine (3TC, 50 µM); raltegravir (Ral, integrase inhibitor, 100 nM); and indinavir (IDV, protease inhibitor, 5 µM). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, *** P < .001, 2-tailed t test.

Journal: The Journal of Infectious Diseases

Article Title: Prevotella timonensis Bacteria Associated With Vaginal Dysbiosis Enhance Human Immunodeficiency Virus Type 1 Susceptibility Of Vaginal CD4 + T Cells

doi: 10.1093/infdis/jiae166

Figure Lengend Snippet: Antiretroviral drugs used in pre-exposure prophylaxis (PrEP) and combination antiretroviral therapy (cART) block Prevotella timonensis –enhanced human immunodeficiency virus type 1 (HIV-1) infection in vaginal CD4 + T cells. A–C , CD4 + T cells isolated from phytohemagglutinin (PHA)–stimulated peripheral blood mononuclear cells (PBMCs) ( A and B ) or vaginal epithelium explants ( C ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d. HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of total cells (CD4 + T cells isolated from PHA-stimulated PBMCs ( A and B ) or CD3 + cells of emigrated fraction (explant model, C ). HIV-1 infection in the presence or absence of the PrEP drug tenofovir (TFV, reverse-transcriptase inhibitor, 50 µM, A , n = 4) or replication inhibitors used in cART ( B , n = 3; C , n = 5); maraviroc (Mar, CCR5 blockage, 30 µM); reverse transcriptase inhibitors zidovudine (ZDV, 20 µM) and lamivudine (3TC, 50 µM); raltegravir (Ral, integrase inhibitor, 100 nM); and indinavir (IDV, protease inhibitor, 5 µM). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, *** P < .001, 2-tailed t test.

Article Snippet: Lactobacillus crispatus (German Collection of Microorganisms and Cell Cultures GmbH [DSMZ]-20584), Lactobacillus iners (DSMZ-13335), Gardnerella vaginalis (DSMZ-4944), Fannyhessea vaginae (DMSZ-15829), Megasphaera elsdenii (DSMZ-20460), Bacteroides fragilis (ATCC-25285), Bacteroides thetaiotaomicron (DSMZ-2079), Escherichia coli (NC0749147), Prevotella amnii (DSMZ-23384), Prevotella bivia (DSMZ-20514), Prevotella copri (or Segatella copri ; DSMZ-18205), Prevotella intermedia (DSMZ-20706), and Prevotella timonensis (or Hoylesella timonensis ; DSMZ-22865) were cultured as recommended by DSMZ.

Techniques: Blocking Assay, Virus, Infection, Isolation, Bacteria, Flow Cytometry, Staining, Reverse Transcription, Protease Inhibitor, Standard Deviation

Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.

Journal: The Journal of Infectious Diseases

Article Title: Prevotella timonensis Bacteria Associated With Vaginal Dysbiosis Enhance Human Immunodeficiency Virus Type 1 Susceptibility Of Vaginal CD4 + T Cells

doi: 10.1093/infdis/jiae166

Figure Lengend Snippet: Prevotella timonensis –induced human immunodeficiency virus type 1 (HIV-1) uptake, fusion, and viral replication in vaginal CD4 + T cells. Vaginal epithelium explants ( A ) or CD4 + T cells isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells ( B–D ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d unless stated differently. A , HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of CD3 + cells of emigrated fraction (explant model, n = 4–8). B , HIV-1 uptake in CD4 + T cells after 4 h HIV-1 exposure as measured by p24 enzyme-linked immunosorbent assay after trypsin treatment and cell lysis (n = 3). C , Pooled data of β-lactamase activity measured by flow cytometry, representing viral fusion upon 4 h infection with NL4.3BaL-BlaM-Vpr (n = 4). D , De novo HIV-1 replication, determined by detecting green fluorescent protein, after HIV-1 NL4.3eGFP-BaL infection (n = 6–7). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, 2-tailed t test.

Article Snippet: Lactobacillus crispatus (German Collection of Microorganisms and Cell Cultures GmbH [DSMZ]-20584), Lactobacillus iners (DSMZ-13335), Gardnerella vaginalis (DSMZ-4944), Fannyhessea vaginae (DMSZ-15829), Megasphaera elsdenii (DSMZ-20460), Bacteroides fragilis (ATCC-25285), Bacteroides thetaiotaomicron (DSMZ-2079), Escherichia coli (NC0749147), Prevotella amnii (DSMZ-23384), Prevotella bivia (DSMZ-20514), Prevotella copri (or Segatella copri ; DSMZ-18205), Prevotella intermedia (DSMZ-20706), and Prevotella timonensis (or Hoylesella timonensis ; DSMZ-22865) were cultured as recommended by DSMZ.

Techniques: Virus, Isolation, Bacteria, Infection, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Lysis, Activity Assay, Standard Deviation

Antiretroviral drugs used in pre-exposure prophylaxis (PrEP) and combination antiretroviral therapy (cART) block Prevotella timonensis –enhanced human immunodeficiency virus type 1 (HIV-1) infection in vaginal CD4 + T cells. A–C , CD4 + T cells isolated from phytohemagglutinin (PHA)–stimulated peripheral blood mononuclear cells (PBMCs) ( A and B ) or vaginal epithelium explants ( C ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d. HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of total cells (CD4 + T cells isolated from PHA-stimulated PBMCs ( A and B ) or CD3 + cells of emigrated fraction (explant model, C ). HIV-1 infection in the presence or absence of the PrEP drug tenofovir (TFV, reverse-transcriptase inhibitor, 50 µM, A , n = 4) or replication inhibitors used in cART ( B , n = 3; C , n = 5); maraviroc (Mar, CCR5 blockage, 30 µM); reverse transcriptase inhibitors zidovudine (ZDV, 20 µM) and lamivudine (3TC, 50 µM); raltegravir (Ral, integrase inhibitor, 100 nM); and indinavir (IDV, protease inhibitor, 5 µM). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, *** P < .001, 2-tailed t test.

Journal: The Journal of Infectious Diseases

Article Title: Prevotella timonensis Bacteria Associated With Vaginal Dysbiosis Enhance Human Immunodeficiency Virus Type 1 Susceptibility Of Vaginal CD4 + T Cells

doi: 10.1093/infdis/jiae166

Figure Lengend Snippet: Antiretroviral drugs used in pre-exposure prophylaxis (PrEP) and combination antiretroviral therapy (cART) block Prevotella timonensis –enhanced human immunodeficiency virus type 1 (HIV-1) infection in vaginal CD4 + T cells. A–C , CD4 + T cells isolated from phytohemagglutinin (PHA)–stimulated peripheral blood mononuclear cells (PBMCs) ( A and B ) or vaginal epithelium explants ( C ) were stimulated overnight by UV-inactivated bacteria ( Lactobacillus crispatus [LC], Lactobacillus iners [LI], Gardnerella vaginalis [GV], Fannyhessea vaginae [FV], Megasphaera elsdenii [ME], and Prevotella timonensis [PT], all on multiplicity of infection [MOI] 10) and subsequently exposed to HIV-1 (SF162; MOI 0.1) for 3 d. HIV-1 infection was measured by flow cytometry after intracellular staining for HIV-1 capsid p24 and depicted here as % p24 + cells of total cells (CD4 + T cells isolated from PHA-stimulated PBMCs ( A and B ) or CD3 + cells of emigrated fraction (explant model, C ). HIV-1 infection in the presence or absence of the PrEP drug tenofovir (TFV, reverse-transcriptase inhibitor, 50 µM, A , n = 4) or replication inhibitors used in cART ( B , n = 3; C , n = 5); maraviroc (Mar, CCR5 blockage, 30 µM); reverse transcriptase inhibitors zidovudine (ZDV, 20 µM) and lamivudine (3TC, 50 µM); raltegravir (Ral, integrase inhibitor, 100 nM); and indinavir (IDV, protease inhibitor, 5 µM). Symbols represent independent donors, bars represent mean ± standard deviation. * P < .05, ** P < .01, *** P < .001, 2-tailed t test.

Article Snippet: Lactobacillus crispatus (German Collection of Microorganisms and Cell Cultures GmbH [DSMZ]-20584), Lactobacillus iners (DSMZ-13335), Gardnerella vaginalis (DSMZ-4944), Fannyhessea vaginae (DMSZ-15829), Megasphaera elsdenii (DSMZ-20460), Bacteroides fragilis (ATCC-25285), Bacteroides thetaiotaomicron (DSMZ-2079), Escherichia coli (NC0749147), Prevotella amnii (DSMZ-23384), Prevotella bivia (DSMZ-20514), Prevotella copri (or Segatella copri ; DSMZ-18205), Prevotella intermedia (DSMZ-20706), and Prevotella timonensis (or Hoylesella timonensis ; DSMZ-22865) were cultured as recommended by DSMZ.

Techniques: Blocking Assay, Virus, Infection, Isolation, Bacteria, Flow Cytometry, Staining, Reverse Transcription, Protease Inhibitor, Standard Deviation