wt n lactamica  (ATCC)


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    ATCC wt n lactamica
    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. <t>lactamica</t> strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.
    Wt N Lactamica, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri"

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00826-18

    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.
    Figure Legend Snippet: Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.

    Techniques Used:

    n lactamica atcc 49142  (ATCC)


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    ATCC n lactamica atcc 49142
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    n lactamica atcc 49142 pg fragments  (ATCC)


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    ATCC n lactamica atcc 49142 pg fragments
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Pg Fragments, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    n lactamica atcc 49142  (ATCC)


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    ATCC n lactamica atcc 49142
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    n lactamica atcc 49142 ampg  (ATCC)


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    ATCC n lactamica atcc 49142 ampg
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG <t>NL49142</t> in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Ampg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    Polymorphism of N. lactamica AmpG contributes to the differences in the amounts of PG monomer released. A) N. gonorrhoeae lacking gonococcal ampG and instead expressing ampG NL49142 with a single substitution at AmpGNL49142 residue 272 from a threonine to an alanine shows reduced PG monomer release by approximately half. B) N. gonorrhoeae expressing ampG NL23970 with a single substitution at AmpGNL23970 residue 272 from an alanine to a threonine released N. gonorrhoeae WT- like levels of PG monomer.
    Figure Legend Snippet: Polymorphism of N. lactamica AmpG contributes to the differences in the amounts of PG monomer released. A) N. gonorrhoeae lacking gonococcal ampG and instead expressing ampG NL49142 with a single substitution at AmpGNL49142 residue 272 from a threonine to an alanine shows reduced PG monomer release by approximately half. B) N. gonorrhoeae expressing ampG NL23970 with a single substitution at AmpGNL23970 residue 272 from an alanine to a threonine released N. gonorrhoeae WT- like levels of PG monomer.

    Techniques Used: Expressing, Residue

    n lactamica atcc 49142 chromosomal dna  (ATCC)


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    ATCC n lactamica atcc 49142 chromosomal dna
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Chromosomal Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    n lactamica atcc 49142  (ATCC)


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    ATCC n lactamica atcc 49142
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals"

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    Journal: bioRxiv

    doi: 10.1101/2024.01.09.574863

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Figure Legend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Techniques Used: Expressing

    wt n lactamica  (ATCC)


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    ATCC wt n lactamica
    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. <t>lactamica</t> strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.
    Wt N Lactamica, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri"

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00826-18

    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.
    Figure Legend Snippet: Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.

    Techniques Used:

    y 2016 who reference strain 33 who z 2016 who reference strain 33 n lactamica 49142 wt n lactamica  (ATCC)


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    ATCC y 2016 who reference strain 33 who z 2016 who reference strain 33 n lactamica 49142 wt n lactamica
    Y 2016 Who Reference Strain 33 Who Z 2016 Who Reference Strain 33 N Lactamica 49142 Wt N Lactamica, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l gasseri 33323 wt l gasseri  (ATCC)


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    ATCC l gasseri 33323 wt l gasseri
    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. <t>gasseri</t> <t>33323</t> was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.
    L Gasseri 33323 Wt L Gasseri, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri"

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00826-18

    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.
    Figure Legend Snippet: Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.

    Techniques Used:

    l gasseri 33323 wt l gasseri  (ATCC)


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    ATCC l gasseri 33323 wt l gasseri
    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. <t>gasseri</t> <t>33323</t> was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.
    L Gasseri 33323 Wt L Gasseri, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri"

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00826-18

    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.
    Figure Legend Snippet: Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.

    Techniques Used:

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  • 94
    ATCC wt n lactamica
    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. <t>lactamica</t> strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.
    Wt N Lactamica, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC n lactamica atcc 49142
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC n lactamica atcc 49142 pg fragments
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Pg Fragments, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC n lactamica atcc 49142 ampg
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG <t>NL49142</t> in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Ampg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC n lactamica atcc 49142 chromosomal dna
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    N Lactamica Atcc 49142 Chromosomal Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC y 2016 who reference strain 33 who z 2016 who reference strain 33 n lactamica 49142 wt n lactamica
    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica <t>ATCC</t> <t>49142</t> or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.
    Y 2016 Who Reference Strain 33 Who Z 2016 Who Reference Strain 33 N Lactamica 49142 Wt N Lactamica, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC l gasseri 33323 wt l gasseri
    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. <t>gasseri</t> <t>33323</t> was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.
    L Gasseri 33323 Wt L Gasseri, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    doi: 10.1128/AAC.00826-18

    Figure Lengend Snippet: Growth of Neisseria species are inhibited by DTZ. N. gonorrhoeae strain MS11 (A to C) and N. lactamica strain 49142 (D) were grown in an automatic plate reader for 14 to 16 h with DTZ of indicated concentrations either added 2 h into growth (mid-log-phase) (A and D), added 4.5 h into growth (late log phase) (B), or present throughout growth (C). Dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of three times. Error bars represent means ± standard deviations (SD) from three replicate wells of a single representative experiment.

    Article Snippet: 49142 , WT N. lactamica (ATCC 49142) , 61.

    Techniques:

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Journal: bioRxiv

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    doi: 10.1101/2024.01.09.574863

    Figure Lengend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Article Snippet: AmpG residue 272 is a threonine in N. lactamica ATCC 49142 and an alanine in N. lactamica ATCC 23970, and WT N. gonorrhoeae and WT N. meningitidis .

    Techniques: Expressing

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Journal: bioRxiv

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    doi: 10.1101/2024.01.09.574863

    Figure Lengend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Article Snippet: By contrast, N. lactamica ATCC 49142 PG fragments released were made up of PG monomers and free peptides in approximately equal amounts, similar to PG fragment release seen with N. gonorrhoeae ( ).

    Techniques: Expressing

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Journal: bioRxiv

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    doi: 10.1101/2024.01.09.574863

    Figure Lengend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Article Snippet: As an alternative strategy, we expressed N. lactamica ATCC 23970 ampG ( ampG NL23970) or N. lactamica ATCC 49142 ampG ( ampG NL49142) in N. gonorrhoeae in lieu of the native gonococcal ampG ( ampG GC).

    Techniques: Expressing

    Polymorphism of N. lactamica AmpG contributes to the differences in the amounts of PG monomer released. A) N. gonorrhoeae lacking gonococcal ampG and instead expressing ampG NL49142 with a single substitution at AmpGNL49142 residue 272 from a threonine to an alanine shows reduced PG monomer release by approximately half. B) N. gonorrhoeae expressing ampG NL23970 with a single substitution at AmpGNL23970 residue 272 from an alanine to a threonine released N. gonorrhoeae WT- like levels of PG monomer.

    Journal: bioRxiv

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    doi: 10.1101/2024.01.09.574863

    Figure Lengend Snippet: Polymorphism of N. lactamica AmpG contributes to the differences in the amounts of PG monomer released. A) N. gonorrhoeae lacking gonococcal ampG and instead expressing ampG NL49142 with a single substitution at AmpGNL49142 residue 272 from a threonine to an alanine shows reduced PG monomer release by approximately half. B) N. gonorrhoeae expressing ampG NL23970 with a single substitution at AmpGNL23970 residue 272 from an alanine to a threonine released N. gonorrhoeae WT- like levels of PG monomer.

    Article Snippet: As an alternative strategy, we expressed N. lactamica ATCC 23970 ampG ( ampG NL23970) or N. lactamica ATCC 49142 ampG ( ampG NL49142) in N. gonorrhoeae in lieu of the native gonococcal ampG ( ampG GC).

    Techniques: Expressing, Residue

    Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Journal: bioRxiv

    Article Title: Peptidoglycan fragment release and NOD activation by commensal Neisseria species from humans and other animals

    doi: 10.1101/2024.01.09.574863

    Figure Lengend Snippet: Gonococcal ampG - strains expressing ampG from two different strains of N. lactamica released different amounts of PG monomer. The ampG gene from N. lactamica ATCC 49142 or N. lactamica ATCC 23970 were expressed in N. gonorrhoeae in lieu of the native gonococcal ampG gene. Expression of ampG NL23970 but not ampG NL49142 in N. gonorrhoeae reduced the amount of PG monomer released.

    Article Snippet: First, ampG NL49142 was amplified from N. lactamica ATCC 49142 chromosomal DNA using primers SOE-NL ampG F and SOE-NL ampG R. Approximately 1kb ampG GC upstream and downstream regions, which include the native ampG GC promoter and transcriptional terminator, were amplified from N. gonorrhoeae MS11 chromosomal DNA using primer pairs MC ampG SacI F3/NL SOE-MS11 ampG 5’ flank R and NL SOE-MS11 ampG 3’ flank F/ ampG 3’ flank R BamHI, respectively.

    Techniques: Expressing

    Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri

    doi: 10.1128/AAC.00826-18

    Figure Lengend Snippet: Growth of L. gasseri is not inhibited by DTZ at concentrations inhibitory to N. gonorrhoeae. (A to C) L. gasseri 33323 was grown in an automatic plate reader for 16 h with DTZ of the indicated concentrations either added 90 min into growth (early log phase) (A), added 6 h into growth (late log phase) (B), or present throughout growth (C). The dotted vertical line indicates time of treatment, vehicle is 30% DMSO. All conditions were assessed in triplicate technical replicate wells, and each experiment was repeated a minimum of two times. Error bars represent means ± SD from three replicate wells of a single representative experiment.

    Article Snippet: The dithiazoline compound JNJ-853346 (DTZ) was provided as a lyophilized powder and suspended at a stock concentration of 10 mM in 30% dimethyl sulfoxide (DMSO) ( 17 ). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Description Reference or source N. gonorrhoeae MS11 WT N. gonorrhoeae 56 KH626 MS11 mtrD ::kan This work JL539 MS11 Δ ldcA 22 JL540 MS11 Δ ldcA ldcA + 22 F62 WT N. gonorrhoeae 53 FA1090 WT N. gonorrhoeae 57 FA19 WT N. gonorrhoeae 58 DGI-2 Disseminated gonococcal infection patient isolate, blood 59 DGI-18 Disseminated gonococcal infection patient isolate, blood and cervix 59 SK-92-679 Disseminated gonococcal infection patient isolate, blood 60 SK-93-1035 Disseminated gonococcal infection patient isolate, blood 60 PID-1 Pelvic inflammatory disease patient isolate, cervix 59 PID24-1 Pelvic inflammatory disease patient isolate, cervix 59 PID332 Pelvic inflammatory disease patient isolate, cervix 59 WHO F 2016 WHO reference strain 34 WHO G 2016 WHO reference strain 34 WHO K 2016 WHO reference strain 34 WHO L 2016 WHO reference strain 34 WHO O 2016 WHO reference strain 34 WHO P 2016 WHO reference strain 34 WHO U 2016 WHO reference strain 34 WHO V 2016 WHO reference strain 34 WHO W 2016 WHO reference strain 34 WHO X 2016 WHO reference strain 34 WHO Y 2016 WHO reference strain 34 WHO Z 2016 WHO reference strain 34 N. lactamica 49142 WT N. lactamica (ATCC 49142) 61 L. gasseri 33323 WT L. gasseri (ATCC 33323) 28 Open in a separate window Strains used in this study Growth curves.

    Techniques: