c cynodegmi (ATCC)


Structured Review

C Cynodegmi, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c cynodegmi/product/ATCC
Average 93 stars, based on 1 article reviews
Images
1) Product Images from "A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi"
Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi
Journal: Microbiology Spectrum
doi: 10.1128/spectrum.02916-22

Figure Legend Snippet: PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. cynodegmi belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
Techniques Used: Amplification, Agarose Gel Electrophoresis, Molecular Weight, Marker

Figure Legend Snippet: Newly designed 16S rRNA PCR-RFLP for identifying the Capnocytophaga species and isolates obtained in this study
Techniques Used:

Figure Legend Snippet: Phylogenetic trees were constructed based on approximately 440 bp of the 16S rRNA sequence of published C. canimorsus and C. cynodegmi strains. (A) Published C. canimorsus strains. Strains without the expected restriction enzyme (RE) sequences were clustered in a separate clade, except for two strains (indicated by filled triangles). (B) Published C. cynodegmi strains. Strains without the expected RE sequences were clustered in a clade that was in close proximity to other clades. Filled dots indicate strains without the expected RE sequence.
Techniques Used: Construct, Sequencing

Figure Legend Snippet: Molecular techniques for the detection of C. canimorsus - and C. cynodegmi -related species
Techniques Used: