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c cynodegmi  (ATCC)


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    Structured Review

    ATCC c cynodegmi
    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. <t>cynodegmi</t> belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
    C Cynodegmi, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c cynodegmi/product/ATCC
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    c cynodegmi - by Bioz Stars, 2025-04
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    Images

    1) Product Images from "A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi"

    Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.02916-22

    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. cynodegmi belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
    Figure Legend Snippet: PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. cynodegmi belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.

    Techniques Used: Amplification, Agarose Gel Electrophoresis, Molecular Weight, Marker

    Newly designed 16S rRNA PCR-RFLP for identifying the Capnocytophaga species and isolates obtained in this study
    Figure Legend Snippet: Newly designed 16S rRNA PCR-RFLP for identifying the Capnocytophaga species and isolates obtained in this study

    Techniques Used:

    Phylogenetic trees were constructed based on approximately 440 bp of the 16S rRNA sequence of published C. canimorsus and C. cynodegmi strains. (A) Published C. canimorsus strains. Strains without the expected restriction enzyme (RE) sequences were clustered in a separate clade, except for two strains (indicated by filled triangles). (B) Published C. cynodegmi strains. Strains without the expected RE sequences were clustered in a clade that was in close proximity to other clades. Filled dots indicate strains without the expected RE sequence.
    Figure Legend Snippet: Phylogenetic trees were constructed based on approximately 440 bp of the 16S rRNA sequence of published C. canimorsus and C. cynodegmi strains. (A) Published C. canimorsus strains. Strains without the expected restriction enzyme (RE) sequences were clustered in a separate clade, except for two strains (indicated by filled triangles). (B) Published C. cynodegmi strains. Strains without the expected RE sequences were clustered in a clade that was in close proximity to other clades. Filled dots indicate strains without the expected RE sequence.

    Techniques Used: Construct, Sequencing

    Molecular techniques for the detection of C. canimorsus - and  C. cynodegmi  -related species <xref ref-type= a " title="... for the detection of C. canimorsus - and C. cynodegmi -related species ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Molecular techniques for the detection of C. canimorsus - and C. cynodegmi -related species a

    Techniques Used:



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    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. <t>cynodegmi</t> belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
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    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. <t>cynodegmi</t> belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
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    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. <t>cynodegmi</t> belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.
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    Image Search Results


    PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. cynodegmi belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.

    Journal: Microbiology Spectrum

    Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi

    doi: 10.1128/spectrum.02916-22

    Figure Lengend Snippet: PpuMI and KpnI restriction patterns for the digestion of amplified 16S rRNA gene products. DNA fragments were analyzed by 1.5% agarose gel electrophoresis. Small molecular weight fragments (<100 bp) are unrecognizable in the image. M, 100-bp marker; Ccy1, C. cynodegmi belonging to RFLP pattern type 2 (478 and 987 bp); Ccy2, C. cynodegmi belonging to RFLP pattern type 3 (51, 478, and 936 bp); Cca, C. canimorsus belonging to RFLP pattern type 1 (14, 51, 286, and 1,114 bp); C. sp.: strains 62067N and 91462, the suspected new Capnocytophaga species, belonging to RFLP pattern type 4 (51 and 1,414 bp); P, undigested PCR-amplified 16S rRNA product.

    Article Snippet: The reference strains retrieved from GenBank included three strains of C. canimorsus (ATCC 35979, CP022382 , and NC015846 ), three strains of C. cynodegmi (ATCC 49044, AY643076 , and CP022378 ), one strain of C. felis (accession no. LC411961 ), one strain of C. canis (accession no. LC100036.1 ), and one strain of C. stomatis ( CP022387 ).

    Techniques: Amplification, Agarose Gel Electrophoresis, Molecular Weight, Marker

    Newly designed 16S rRNA PCR-RFLP for identifying the Capnocytophaga species and isolates obtained in this study

    Journal: Microbiology Spectrum

    Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi

    doi: 10.1128/spectrum.02916-22

    Figure Lengend Snippet: Newly designed 16S rRNA PCR-RFLP for identifying the Capnocytophaga species and isolates obtained in this study

    Article Snippet: The reference strains retrieved from GenBank included three strains of C. canimorsus (ATCC 35979, CP022382 , and NC015846 ), three strains of C. cynodegmi (ATCC 49044, AY643076 , and CP022378 ), one strain of C. felis (accession no. LC411961 ), one strain of C. canis (accession no. LC100036.1 ), and one strain of C. stomatis ( CP022387 ).

    Techniques:

    Phylogenetic trees were constructed based on approximately 440 bp of the 16S rRNA sequence of published C. canimorsus and C. cynodegmi strains. (A) Published C. canimorsus strains. Strains without the expected restriction enzyme (RE) sequences were clustered in a separate clade, except for two strains (indicated by filled triangles). (B) Published C. cynodegmi strains. Strains without the expected RE sequences were clustered in a clade that was in close proximity to other clades. Filled dots indicate strains without the expected RE sequence.

    Journal: Microbiology Spectrum

    Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi

    doi: 10.1128/spectrum.02916-22

    Figure Lengend Snippet: Phylogenetic trees were constructed based on approximately 440 bp of the 16S rRNA sequence of published C. canimorsus and C. cynodegmi strains. (A) Published C. canimorsus strains. Strains without the expected restriction enzyme (RE) sequences were clustered in a separate clade, except for two strains (indicated by filled triangles). (B) Published C. cynodegmi strains. Strains without the expected RE sequences were clustered in a clade that was in close proximity to other clades. Filled dots indicate strains without the expected RE sequence.

    Article Snippet: The reference strains retrieved from GenBank included three strains of C. canimorsus (ATCC 35979, CP022382 , and NC015846 ), three strains of C. cynodegmi (ATCC 49044, AY643076 , and CP022378 ), one strain of C. felis (accession no. LC411961 ), one strain of C. canis (accession no. LC100036.1 ), and one strain of C. stomatis ( CP022387 ).

    Techniques: Construct, Sequencing

    Molecular techniques for the detection of C. canimorsus - and  C. cynodegmi  -related species <xref ref-type= a " width="100%" height="100%">

    Journal: Microbiology Spectrum

    Article Title: A Novel 16S rRNA PCR-Restriction Fragment Length Polymorphism Assay to Accurately Distinguish Zoonotic Capnocytophaga canimorsus and C. cynodegmi

    doi: 10.1128/spectrum.02916-22

    Figure Lengend Snippet: Molecular techniques for the detection of C. canimorsus - and C. cynodegmi -related species a

    Article Snippet: The reference strains retrieved from GenBank included three strains of C. canimorsus (ATCC 35979, CP022382 , and NC015846 ), three strains of C. cynodegmi (ATCC 49044, AY643076 , and CP022378 ), one strain of C. felis (accession no. LC411961 ), one strain of C. canis (accession no. LC100036.1 ), and one strain of C. stomatis ( CP022387 ).

    Techniques:

    Pistillate flowers of A Cissampelos arenicola M. Nee & R. Ortiz, ( M. Nee 49044 , MO) B Cissampelos capensis L. f., ( S.L. Williams 295 , MO) showing locations of sepals and petals; s = sepal; p = petal; c = carpel; as = adaxial suture.

    Journal: PhytoKeys

    Article Title: A new species of Cissampelos (Menispermaceae) from Bolivia and Paraguay

    doi: 10.3897/phytokeys.38.6504

    Figure Lengend Snippet: Pistillate flowers of A Cissampelos arenicola M. Nee & R. Ortiz, ( M. Nee 49044 , MO) B Cissampelos capensis L. f., ( S.L. Williams 295 , MO) showing locations of sepals and petals; s = sepal; p = petal; c = carpel; as = adaxial suture.

    Article Snippet: Andrés Ibáñez, along Quebrada Peji, vic. bridge of new hwy from Santa Cruz to Camiri and railroad bridge, 17°57'30"S, 63°11'00"W , 440 m, 11 Dec 1994 (imm & mat fr), Nee 45861 (LPB, NY!, USZ); along hwy from Santa Cruz to Abapó, 3 km S of crossing of railroad and 2 km S of bridge over Quebrada Peji, 17°58'S, 63°11.3'W , 450 m, 27 Feb 1998 (♂ fl, imm fr), Nee et al. 48487 (LPB, NY!, USZ); 15 Nov 2000 (♂ fl, imm fr), Nee 51401 (NY); along hwy from Santa Cruz to Abapó, 5.4 km S of turnoff at “km 13”, 17°55'S, 63°15'W , 470 m, 18 Apr 1998 (imm fr), Nee 49044 (LPB, NY!, USZ); Prov.

    Techniques: