proteus mirabilis cmcc 49005  (ATCC)

Journal: Cell reports

Article Title: Cells and circuits for amygdala neuroplasticity in the transition to chronic pain

doi: 10.1016/j.celrep.2024.114669

Figure Lengend Snippet:

Article Snippet: Whole-cell patch-clamp recordings were obtained from visually identified mCherry-expressing CRF neurons in the CeL/C, mCherry-negative non-CRF neurons in the CeC, and tdTomato- or EGFP-expressing CRF neurons in the CeL/C of the right hemisphere, using LED illumination system (X-Cite 120 LED, Excelitas Technologies Corp.) with ET-DSRed (TRITC/Cy3) (49005, Chroma Technology Corp.) (for mCherry and tdTomato) and ET-EGFP (FITC/Cy2) (49002, Chroma Technology Corp.) (for EGFP) filter sets, and infrared DIC videomicroscopy as described previously., Recording electrodes (tip resistance 5–8 MΩ) were made from borosilicate glass and filled with intracellular solution containing (in mM): 122 K-gluconate, 5 NaCl, 0.3 CaCl 2 , 2 MgCl 2 , 1 EGTA, 10 HEPES, 5 Na 2 -ATP, and 0.4 Na 3 -GTP; pH was adjusted to 7.2–7.3 with KOH and osmolarity to 280 mOsm/kg with sucrose.

Techniques: Virus, Plasmid Preparation, Recombinant, Software