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luciferase assay kit  (Biothema AB)


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    Structured Review

    Biothema AB luciferase assay kit
    Luciferase Assay Kit, supplied by Biothema AB, used in various techniques. Bioz Stars score: 91/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/484-001/Luciferase+assay+Kit/pm37164185-98-12-15
    Average 91 stars, based on 81 article reviews
    luciferase assay kit - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Luciferase:

    Article Title: Benzo[b]naphtho[d]thiophenes and naphthylbenzo[b]thiophenes: Their aryl hydrocarbon receptor-mediated activities and environmental presence.
    Article Snippet: ⁎ Corresponding authors.. E-mail addresses: vondracek@ibp.cz (J. Vondráček), mir 1 Present address: Central European Institute of Technolo http://dx.doi.org/10.1016/j.scitotenv.2023.162924 Received 19 December 2022; Received in revised for Available online 16 March 2023 0048-9697/© 2023 Elsevier B.V. All rights reserved.. • Several PASHs were newly identified as rodent and human AhR agonists.

    Article Title: CC chemokine ligand 2 (CCL2) stimulates aromatase gene expression in mammary adipose tissue
    Article Snippet: .. Luc activity was measured from the supernatant with the Luciferase assay kit (BioThema AB, Handen, Sweden) according to manufacturer's instructions by using the Victor2 Multilabel counter (PerkinElmer, Turku, Finland). .. The results were related to the sample weight or protein content measured by using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) according to manufacturer's instructions.

    Article Title: In vitro profiling of toxic effects of environmental polycyclic aromatic hydrocarbons on nuclear receptor signaling, disruption of endogenous metabolism and induction of cellular stress.
    Article Snippet: • Novel toxic effects of environmental PAHs were screened in human in vitro models.. • Low-molecular-weight PAHs activated/ enhanced ERalpha, TRalpha and CAR re-

    Article Title: Hypoxia-inducible factor-1α and poly [ADP ribose] polymerase 1 cooperatively regulate Notch3 expression under hypoxia via a noncanonical mechanism
    Article Snippet: After growth, the cells were lysed using cell culture lysis buffer (Promega), according to the manufacturer’s instructions. .. Total cell lysates were used to measure luciferase activity using GloMax (Promega) with a Luciferase assay kit (BioThema). .. Protein concentrations of lysates were determined to normalize luciferase activities by the Bradford method (Bio-Rad).

    Article Title: Characterization of elements, PAHs, AhR-activity and pro-inflammatory responses of road tunnel-derived particulate matter in human hepatocyte-like and bronchial epithelial cells.
    Article Snippet: The aims were to characterize the content of elements and polycyclic aromatic hydrocarbons (PAHs) in sizeseparated particulate matter (PM) sampled in a road tunnel, estimate the contribution of PAHs to the toxic potential, and measure the pro-inflammatory potential of PM samples and extracts with increasing polarity.. Several elements/metals previously associated with cytokine responses were found.. Based on PAHs levels and published PAHs potency, the calculated mutagenic and carcinogenic activities of size-separated samples were somewhat lower for coarse than fine and ultrafine PM.

    Activity Assay:

    Article Title: Benzo[b]naphtho[d]thiophenes and naphthylbenzo[b]thiophenes: Their aryl hydrocarbon receptor-mediated activities and environmental presence.
    Article Snippet: ⁎ Corresponding authors.. E-mail addresses: vondracek@ibp.cz (J. Vondráček), mir 1 Present address: Central European Institute of Technolo http://dx.doi.org/10.1016/j.scitotenv.2023.162924 Received 19 December 2022; Received in revised for Available online 16 March 2023 0048-9697/© 2023 Elsevier B.V. All rights reserved.. • Several PASHs were newly identified as rodent and human AhR agonists.

    Article Title: CC chemokine ligand 2 (CCL2) stimulates aromatase gene expression in mammary adipose tissue
    Article Snippet: .. Luc activity was measured from the supernatant with the Luciferase assay kit (BioThema AB, Handen, Sweden) according to manufacturer's instructions by using the Victor2 Multilabel counter (PerkinElmer, Turku, Finland). .. The results were related to the sample weight or protein content measured by using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) according to manufacturer's instructions.

    Article Title: In vitro profiling of toxic effects of environmental polycyclic aromatic hydrocarbons on nuclear receptor signaling, disruption of endogenous metabolism and induction of cellular stress.
    Article Snippet: • Novel toxic effects of environmental PAHs were screened in human in vitro models.. • Low-molecular-weight PAHs activated/ enhanced ERalpha, TRalpha and CAR re-

    Article Title: Hypoxia-inducible factor-1α and poly [ADP ribose] polymerase 1 cooperatively regulate Notch3 expression under hypoxia via a noncanonical mechanism
    Article Snippet: After growth, the cells were lysed using cell culture lysis buffer (Promega), according to the manufacturer’s instructions. .. Total cell lysates were used to measure luciferase activity using GloMax (Promega) with a Luciferase assay kit (BioThema). .. Protein concentrations of lysates were determined to normalize luciferase activities by the Bradford method (Bio-Rad).

    Article Title: Characterization of elements, PAHs, AhR-activity and pro-inflammatory responses of road tunnel-derived particulate matter in human hepatocyte-like and bronchial epithelial cells.
    Article Snippet: The aims were to characterize the content of elements and polycyclic aromatic hydrocarbons (PAHs) in sizeseparated particulate matter (PM) sampled in a road tunnel, estimate the contribution of PAHs to the toxic potential, and measure the pro-inflammatory potential of PM samples and extracts with increasing polarity.. Several elements/metals previously associated with cytokine responses were found.. Based on PAHs levels and published PAHs potency, the calculated mutagenic and carcinogenic activities of size-separated samples were somewhat lower for coarse than fine and ultrafine PM.

    other:

    Article Title: miR-181b targets semaphorin 3A to mediate TGF-β–induced endothelial-mesenchymal transition related to atrial fibrillation
    Article Snippet: Site-directed mutagenesis of the putative miR-181b binding sites 226 was performed by PCR using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, 227 catalog E0554, Ipswich, MA).

    Article Title: miR-181b targets semaphorin 3A to mediate TGF-β–induced endothelial-mesenchymal transition related to atrial fibrillation
    Article Snippet: Site-directed mutagenesis of the SBEs was performed by 239 PCR using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, catalog E0554).



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