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Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, <t>ANXA2</t> and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)
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https://www.bioz.com/result/anti anxa2 mouse monoclonal antibody/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
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anxa2 antibody  (Santa Cruz Biotechnology)
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Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, <t>ANXA2</t> and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)
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annexin a2 mouse monoclonal antibody  (Santa Cruz Biotechnology)
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Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, <t>ANXA2</t> and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)
Annexin A2 Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin a2 mouse monoclonal antibody/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
annexin a2 mouse monoclonal antibody - by Bioz Stars, 2025-06
94/100 stars
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Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, ANXA2 and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)

Journal: BMC Genomics

Article Title: Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

doi: 10.1186/s12864-024-10429-5

Figure Lengend Snippet: Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, ANXA2 and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)

Article Snippet: Antibodies and their dilutions used for Western blotting experiments were as follows: primary antibodies: Anti-CD9 Mouse Monoclonal Antibody (Clone MM2/57, MCA469GT, Bio-Rad), 1:500; Anti-HSP70 Mouse Monoclonal Antibody (Santa Cruz sc-66048), 1:500; Anti-ANXA2 Mouse Monoclonal Antibody (Santa Cruz sc-28385), 1:500; secondary antibody: Anti-mouse m-IgGκ BP-HRP (Santa Cruz sc-516102), 1:10000.

Techniques: Transmission Assay, Electron Microscopy, Isolation, Cell Culture, Expressing, Flow Cytometry, Western Blot, Centrifugation, Comparison, Concentration Assay