Journal: BMC Geriatrics

Article Title: Elevated hydrogen peroxide and decreased catalase and glutathione peroxidase protection are associated with aging sarcopenia

doi: 10.1186/1471-2318-13-104

Figure Lengend Snippet: Relative gene expression in aging skeletal muscle

Article Snippet: The membranes were blocked for 2 hours at room temperature in Tris-buffered saline containing Tris-HCl (20 mM; pH 7.6) NaCl (137 mM) and Tween 20 (0.1%) with 5% BSA and probed with primary antibodies for either NOX2 (gp91 phox ), p40 phox , p67 phox or GAPDH (1:200; SantaCruz Biotechnology) overnight at 4°C.

Techniques: Expressing

Journal: BMC Geriatrics

Article Title: Elevated hydrogen peroxide and decreased catalase and glutathione peroxidase protection are associated with aging sarcopenia

doi: 10.1186/1471-2318-13-104

Figure Lengend Snippet: The protein levels of the NOX2 enzyme subunits and protein levels in aging skeletal muscle . A . Representative blots for NOX2, p40 phox , p67 phox and GAPDH. B . Graphical representation of protein concentration expressed as arbitrary units (AU) after normalisation for GAPDH concentration. The values represent the mean ± SEM. Statistically significant differences (P < 0.05) between groups are indicated by lower case letters, where a = 6 m/o, b = 12 m/o, c = 18 m/o and d = 24 m/o.

Article Snippet: The membranes were blocked for 2 hours at room temperature in Tris-buffered saline containing Tris-HCl (20 mM; pH 7.6) NaCl (137 mM) and Tween 20 (0.1%) with 5% BSA and probed with primary antibodies for either NOX2 (gp91 phox ), p40 phox , p67 phox or GAPDH (1:200; SantaCruz Biotechnology) overnight at 4°C.

Techniques: Protein Concentration, Concentration Assay

Journal: PLoS ONE

Article Title: Urotensin II Inhibits Skeletal Muscle Glucose Transport Signaling Pathways via the NADPH Oxidase Pathway

doi: 10.1371/journal.pone.0076796

Figure Lengend Snippet: KK mice were administrated urantide (30 µg/kg/day) for 14 days prior to western blot analysis with antibodies against NOX2, p67-phox, p47-phox, p40 phox and p22 phox . Equal loading was confirmed by reprobing the membranes with GAPDH antibody. Control: C57BL/6J mice were treated with saline for 14 days; Urantide: C57BL/6J mice were treated with urantide for 14 days; KK:KK mice were treated with saline for 14 days; KK+Urantide: KK mice were treated with urantide for 14 days. Data are presented as the relative increase compared with control (100%; n=3, * P <0.05 vs control; # P <0.05 vs KK group).

Article Snippet: Anti-gp91-phox, anti-p67-phox, anti-p47-phox, anti-p40-phox, anti-p22-phox and GLUT4 were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Urotensin II Inhibits Skeletal Muscle Glucose Transport Signaling Pathways via the NADPH Oxidase Pathway

doi: 10.1371/journal.pone.0076796

Figure Lengend Snippet: C2C12 cells were stimulated with 100 nM UII for 2h after treatment with apocynin for 30 minutes. Western blot analysis was performed with antibodies against NOX2, p67-phox, p47-phox, p40 phox and p22 phox . Equal loading was confirmed by reprobing the membranes with GAPDH antibody. Control: cells were incubated with fresh FCS free DMEM for 24h; UII: cells were treated with UII (100 nmol/L) for 24h. Data are presented as the relative increase compared with control (100%; n=3, * P <0.05 vs group; # P <0.05 vs UII group).

Article Snippet: Anti-gp91-phox, anti-p67-phox, anti-p47-phox, anti-p40-phox, anti-p22-phox and GLUT4 were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, Incubation

Journal: Cell reports

Article Title: LACC1 Required for NOD2-Induced, ER Stress-Mediated Innate Immune Outcomes in Human Macrophages and LACC1 Risk Variants Modulate These Outcomes

doi: 10.1016/j.celrep.2019.11.105

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal p40-phox (D-8) , Santa Cruz Biotechnology , Cat# sc-48388.

Techniques: FLAG-tag, Recombinant, Transfection, Software