Journal: Communications Biology

Article Title: The role of PD-1/PD-L1 in overshooting osteoclastogenesis in periprosthetic joint infections

doi: 10.1038/s42003-025-08143-3

Figure Lengend Snippet: A TRAP staining for osteoclasts. Scale bar: 500 µm. B Histomorphometric quantification of osteoclast-related parameters. Oc.N/BS: Number of osteoclasts per bone surface; Oc.S/BS: Osteoclast surface per bone surface. C Distribution of CTSK and CD68 positive cells in the trabecular bone structure. Scale bar: 500 µm. D Quantification of CTSK positive (osteoclasts) and CD68 positive (macrophages) cells.

Article Snippet: For immunofluorescence, frozen sections were treated with Tris-Buffered Saline, permeabilized by 0.25% Triton/PBS solution for 10 min, and then blocked in 5% goat serum/5% donkey serum/3% BSA/PBS before overnight incubation with primary antibodies (anti- Cathepsin K (CTSK), 1:400, mouse monoclonal, sc-48353, Santa Cruz, USA; anti-CD68, 1:500, HPA048982, Atlas Antibodies AB, USA).

Techniques: Staining

Journal: Communications Biology

Article Title: The role of PD-1/PD-L1 in overshooting osteoclastogenesis in periprosthetic joint infections

doi: 10.1038/s42003-025-08143-3

Figure Lengend Snippet: A - D TRAP activity and CTSK concentration in cell medium and intracellularly. E Toluidine blue staining of pit formation assay and SEM imaging of bone slices following in vitro osteoclast culture. Scale bar for pit assay: 500 µm, for SEM: 100 µm. F Quantification of pit formation assay.

Article Snippet: For immunofluorescence, frozen sections were treated with Tris-Buffered Saline, permeabilized by 0.25% Triton/PBS solution for 10 min, and then blocked in 5% goat serum/5% donkey serum/3% BSA/PBS before overnight incubation with primary antibodies (anti- Cathepsin K (CTSK), 1:400, mouse monoclonal, sc-48353, Santa Cruz, USA; anti-CD68, 1:500, HPA048982, Atlas Antibodies AB, USA).

Techniques: Activity Assay, Concentration Assay, Staining, Tube Formation Assay, Imaging, In Vitro