Journal: BMC Oral Health
Article Title: ZEB1 expression in Th17 cells correlated with p-STAT3 in human apical periodontitis
doi: 10.1186/s12903-025-05633-y
Figure Lengend Snippet: Immunofluorescence colocalization of ZEB1 with IL-17 and CD4 in human periapical tissues. (A) ZEB1-CD4 double labeling in healthy control group, PGs, and RCs. Red , ZEB1; green , IL17; White , CD4; blue , DAPI. Scale bars = 50 μm. (B) ZEB1-IL17/CD4 triple labeling in (a) healthy control group, PGs, and RCs. Red , ZEB1; green , IL17; White , CD4; blue , DAPI. Scale bars = 50 μm. (C) Statistical analysis of ZEB1-CD4 double-positive cells in every group via a box plot. Results are expressed as the number of positive cells per square millimeter. **** p <.0001. The p -value statistically significant when p <.05. (D) Statistical analysis of ZEB1-IL17/CD4 triple positive cells in every group via a box plot. Results are expressed as the number of positive cells per square millimeter. **** p <.0001. The p -value is statistically significant when p <.05. (E) Statistical analysis of ZEB1-positive cells among IL17 + CD4 + Th17 cells in every group via a box plot. Results are expressed as the percentage of positive cells. **** p <.0001. The p -value is statistically significant when p <.05
Article Snippet: The sections were permeabilized with 0.1% Triton X-100 for 15 min. After washing, the sections were blocked with 2.5% bovine serum albumin (BSA) at 37 °C for 30 min and incubated overnight at 4 °C with primary antibodies as follows: rabbit polyclonal antibodies against ZEB1 (1:500; cat. no. SAB5701068; Sigma-Aldrich), rat monoclonal CD4 antibody (1:200; cat. no. sc-13573; Santa Cruz Biotechnology), mouse monoclonal IL-17 antibody (1:200; cat. no. sc-374218; Santa Cruz Biotechnology) and mouse monoclonal p-STAT3 antibody (1:200; cat. no. sc-8059; Santa Cruz Biotechnology).
Techniques: Immunofluorescence, Labeling, Control