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hybri care medium  (ATCC)


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    ATCC hybri care medium
    Hybri Care Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybri care medium/product/ATCC
    Average 95 stars, based on 1 article reviews
    hybri care medium - by Bioz Stars, 2025-02
    95/100 stars

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    Sino Biological stage 46 x laevis embryos
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    ATCC hybri care medium 46 x
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    ScholarOne Inc x 96 dpi page 46
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    Image Search Results


    A) Bright field images of stage 42 X. laevis grown in the presence of DMSO, 100μM Wnt-C59 or 1mM palmostatin. Scale bar=500μm B) Measurements of drug treated stage 42 X. laevis head shape by 3 metrics: distance between eyes, snout length, and overall head area. Mean +/- SEM n>55 *=p<0.05 ***=p<0.001 ****=p<0.0001. All 3 metrics of head shape were significantly altered from DMSO control in both Wnt-C59 and palmostatin treatments. C) Immunofluorescence images of DMSO treated stage 46 X. laevis immunostained for the neuroprogenitor marker nestin and stained with Hoechst to visualize DNA. Scale bar=250μm. D) Quantification of total cell count in forebrains and percentage of forebrain cells positive for nestin signal in stage 46 X. laevis grown in the presence of DMSO, 100μM Wnt-C59 or 1mM palmostatin. Quantifications were performed on maximum projection images from z-stack images of X. laevis brains with a parent-child analysis to determine the number of total cells as determined by Hoechst signal that also were positive for nestin signal. Mean +/- SEM n=12 ***=p<0.001. All Wnt-C59 treated X. laevis died before reaching stage 46 while all palmostatin treated X. laevis display a significantly reduced neuroprogenitor population by nestin positive cell count.

    Journal: bioRxiv

    Article Title: Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA

    doi: 10.1101/2024.10.25.620315

    Figure Lengend Snippet: A) Bright field images of stage 42 X. laevis grown in the presence of DMSO, 100μM Wnt-C59 or 1mM palmostatin. Scale bar=500μm B) Measurements of drug treated stage 42 X. laevis head shape by 3 metrics: distance between eyes, snout length, and overall head area. Mean +/- SEM n>55 *=p<0.05 ***=p<0.001 ****=p<0.0001. All 3 metrics of head shape were significantly altered from DMSO control in both Wnt-C59 and palmostatin treatments. C) Immunofluorescence images of DMSO treated stage 46 X. laevis immunostained for the neuroprogenitor marker nestin and stained with Hoechst to visualize DNA. Scale bar=250μm. D) Quantification of total cell count in forebrains and percentage of forebrain cells positive for nestin signal in stage 46 X. laevis grown in the presence of DMSO, 100μM Wnt-C59 or 1mM palmostatin. Quantifications were performed on maximum projection images from z-stack images of X. laevis brains with a parent-child analysis to determine the number of total cells as determined by Hoechst signal that also were positive for nestin signal. Mean +/- SEM n=12 ***=p<0.001. All Wnt-C59 treated X. laevis died before reaching stage 46 while all palmostatin treated X. laevis display a significantly reduced neuroprogenitor population by nestin positive cell count.

    Article Snippet: Stage 46 X. laevis embryos were whole mount fixed and stained for DNA (Hoechst) and nestin (Rb α-nestin Sino Biological 100244-T08).

    Techniques: Control, Immunofluorescence, Marker, Staining, Cell Counting

    A) Confocal images of stage 42 X. laevis brains from X. laevis grown in the presence of DMSO or 100μM Wnt-C59 immunostained for phospho-histone H3, a marker of actively dividing cells. Scale bar = 250μm. B) Confocal images of stage 42 X. laevis brains from X. laevis expressing importin α modified with a c-terminal CaaX domain which forces cortical localization via farnesylation and grown in the presence of 100μM Wnt-C59 immunostained for phospho-histone H3 and the modified importin α-CaaX construct. C) Confocal images of stage 42 X. laevis brains from X. laevis expressing an mCherry construct modified with a c-terminal CaaX domain and grown in the presence of 100μM Wnt-C59 immunostained for phospho-histone H3 and the modified CaaX construct. D) Quantification of the number of phospho-histone H3 positive cells in stage 42 X. laevis brains of X. laevis grown in the presence of DMSO or 100μM Wnt-C59 and expressing importin α-CaaX or mCherry-CaaX. Mean +/- SEM n>10 *=p<0.05 ****=p<0.0001. Wnt-C59 treated X. laevis showed a significantly reduced number of phospho-histone H3 positive cells in the brain compared to DMSO treated X. laevis . X. laevis expressing importin α-CaaX in the brain display a partial rescue of the reduced phospho-histone H3 levels which was not recapitulated in X . laevis expressing mCherry-CaaX.

    Journal: bioRxiv

    Article Title: Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA

    doi: 10.1101/2024.10.25.620315

    Figure Lengend Snippet: A) Confocal images of stage 42 X. laevis brains from X. laevis grown in the presence of DMSO or 100μM Wnt-C59 immunostained for phospho-histone H3, a marker of actively dividing cells. Scale bar = 250μm. B) Confocal images of stage 42 X. laevis brains from X. laevis expressing importin α modified with a c-terminal CaaX domain which forces cortical localization via farnesylation and grown in the presence of 100μM Wnt-C59 immunostained for phospho-histone H3 and the modified importin α-CaaX construct. C) Confocal images of stage 42 X. laevis brains from X. laevis expressing an mCherry construct modified with a c-terminal CaaX domain and grown in the presence of 100μM Wnt-C59 immunostained for phospho-histone H3 and the modified CaaX construct. D) Quantification of the number of phospho-histone H3 positive cells in stage 42 X. laevis brains of X. laevis grown in the presence of DMSO or 100μM Wnt-C59 and expressing importin α-CaaX or mCherry-CaaX. Mean +/- SEM n>10 *=p<0.05 ****=p<0.0001. Wnt-C59 treated X. laevis showed a significantly reduced number of phospho-histone H3 positive cells in the brain compared to DMSO treated X. laevis . X. laevis expressing importin α-CaaX in the brain display a partial rescue of the reduced phospho-histone H3 levels which was not recapitulated in X . laevis expressing mCherry-CaaX.

    Article Snippet: Stage 46 X. laevis embryos were whole mount fixed and stained for DNA (Hoechst) and nestin (Rb α-nestin Sino Biological 100244-T08).

    Techniques: Marker, Expressing, Modification, Construct