Journal: The Journal of Neuroscience

Article Title: Lack of Evidence for Vesicular Glutamate Transporter Expression in Mouse Astrocytes

doi: 10.1523/JNEUROSCI.3667-12.2013

Figure Lengend Snippet: Combinations of excitation wavelengths and filters

Article Snippet: Antibodies used for fluorescence immunostaining and Western blotting table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Fluorophore ex (nm) DC (nm) em (nm) Confocal microscopy * Alexa 488 488 AOBS BP498-550 Alexa 488 488 HFT/488/543/633 BP505-530 Alexa 546 543 HFT/488/543/633 BP560-615 Alexa 555 561 AOBS BP569-631 Alexa 633 633 HFT/488/543/633 650LP Cy5 633 AOBS BP643-720 TIRFM GFP and FM4-64 488 PolyX † 535(50) and 675(50)BP ‡ Venus and FM4-64 488 PolyX † 535(50) and 675(50)BP ‡ Epifluorescence Spectral imaging of VGLUT1 Venus brain slice 458 ** 500LP 510(20)-535(50)-575(50)-600(40)-670(50)BP Spectral imaging of cultured astrocytes 458 + 500LP 510(20)-535(50)-560(40)-615(45)-670(40)BP Open in a separate window *Acousto-optical beam splitter (AOBS) was used with Leica SP5 confocal microscope and other dichroics with Zeiss LSM 510 confocal microscope.

Techniques: Confocal Microscopy, Imaging, Slice Preparation, Cell Culture