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Administration of anti-SLAMF6 (330) F(ab’) 2 antibody has a similar negative effect on GC B cell and Tfh cell development in protein-immunized WT mice . Mice were immunized with 40 μg of NP-OVA in CFA and some were injected with 250 μg of anti-SLAMF6 F(ab’) 2 on day 0 and day 4. The mice were sacrificed on day 9 and serum was collected to measure IgG production. (A) NP-specific IgG titers were determined by ELISA. (B–C) The percentage and number of Germinal Center B cells (B220 + GL7 + Fas + ) were determined by flow cytometry. (D–E) The percentage and number of Tfh cells (CD4 + PD-1 + CXCR5 + ) were determined by flow cytometry. Results are representative of three independent experiments.
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Administration of anti-SLAMF6 (330) F(ab’) 2 antibody has a similar negative effect on GC B cell and Tfh cell development in protein-immunized WT mice . Mice were immunized with 40 μg of NP-OVA in CFA and some were injected with 250 μg of anti-SLAMF6 F(ab’) 2 on day 0 and day 4. The mice were sacrificed on day 9 and serum was collected to measure IgG production. (A) NP-specific IgG titers were determined by ELISA. (B–C) The percentage and number of Germinal Center B cells (B220 + GL7 + Fas + ) were determined by flow cytometry. (D–E) The percentage and number of Tfh cells (CD4 + PD-1 + CXCR5 + ) were determined by flow cytometry. Results are representative of three independent experiments.

Journal: Frontiers in Immunology

Article Title: Negative Regulation of Humoral Immunity Due to Interplay between the SLAMF1, SLAMF5, and SLAMF6 Receptors

doi: 10.3389/fimmu.2015.00158

Figure Lengend Snippet: Administration of anti-SLAMF6 (330) F(ab’) 2 antibody has a similar negative effect on GC B cell and Tfh cell development in protein-immunized WT mice . Mice were immunized with 40 μg of NP-OVA in CFA and some were injected with 250 μg of anti-SLAMF6 F(ab’) 2 on day 0 and day 4. The mice were sacrificed on day 9 and serum was collected to measure IgG production. (A) NP-specific IgG titers were determined by ELISA. (B–C) The percentage and number of Germinal Center B cells (B220 + GL7 + Fas + ) were determined by flow cytometry. (D–E) The percentage and number of Tfh cells (CD4 + PD-1 + CXCR5 + ) were determined by flow cytometry. Results are representative of three independent experiments.

Article Snippet: Anti-SLAMF6 F(ab’) 2 fragments were generated from whole SLAMF6 mAb, using the Thermo Scientific Pierce F(ab’) 2 Preparation Kit (PI-44988) according to manufacturer instructions.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Flow Cytometry