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p dryinus cbs 44977  (ATCC)


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    ATCC p dryinus cbs 44977
    P Dryinus Cbs 44977, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p dryinus cbs 44977/product/ATCC
    Average 90 stars, based on 2 article reviews
    p dryinus cbs 44977 - by Bioz Stars, 2026-02
    90/100 stars

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    AMXT-1501 increased membrane permeability and depolarization of E. coli . (a) E. coli ECO2219 (CRE) treated with AMXT-1501 for 30 min, labelled with SYTO-9 and PI, and observed under a confocal laser scanning microscope; (b) Fluorescence intensities of PI-labelled E. coli ECO2219 (CRE) treated with AMXT-1501 for 30 min, detected at excitation and emission wavelengths of 535 and 615 nm measured by a microplate reader, respectively. (c) The inner membrane permeabilizing ability of AMXT-1501 was determined by measurement of β-galactosidase activity in E. coli ECO2219 (CRE) using the normally impermeable, chromogenic substrate o-nitrophenyl-β-d-galactoside (ONPG); (d) Fluorescence intensities of DiBAC4(3)-treated E. coli ECO2219 (CRE) treated with AMXT-1501, detected associated fluorescence intensity at 492 and 515 nm for 60 min, respectively. Representative fields are shown in panel a. Data in panels b, c and d were presented as means ± s.d, and P values were determined using an unpaired, two-tailed Student’s t-test. a.u., arbitrary units. AMXT, AMXT-1501; MIC, minimum inhibitory concentration; CRE, carbapenem-resistant Enterobacteriaceae.

    Journal: Emerging Microbes & Infections

    Article Title: AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria

    doi: 10.1080/22221751.2024.2321981

    Figure Lengend Snippet: AMXT-1501 increased membrane permeability and depolarization of E. coli . (a) E. coli ECO2219 (CRE) treated with AMXT-1501 for 30 min, labelled with SYTO-9 and PI, and observed under a confocal laser scanning microscope; (b) Fluorescence intensities of PI-labelled E. coli ECO2219 (CRE) treated with AMXT-1501 for 30 min, detected at excitation and emission wavelengths of 535 and 615 nm measured by a microplate reader, respectively. (c) The inner membrane permeabilizing ability of AMXT-1501 was determined by measurement of β-galactosidase activity in E. coli ECO2219 (CRE) using the normally impermeable, chromogenic substrate o-nitrophenyl-β-d-galactoside (ONPG); (d) Fluorescence intensities of DiBAC4(3)-treated E. coli ECO2219 (CRE) treated with AMXT-1501, detected associated fluorescence intensity at 492 and 515 nm for 60 min, respectively. Representative fields are shown in panel a. Data in panels b, c and d were presented as means ± s.d, and P values were determined using an unpaired, two-tailed Student’s t-test. a.u., arbitrary units. AMXT, AMXT-1501; MIC, minimum inhibitory concentration; CRE, carbapenem-resistant Enterobacteriaceae.

    Article Snippet: CL (841199P; ≥ 99%), phosphatidylcholine (840051P; ≥ 99%) (L130332), phosphatidylethanolamine (840027P; ≥ 99%), PG (841188P; ≥ 99%), LPS ( E. coli ; L2630), peptidoglycan ( S. aureus; 77140), propidium iodide (P4170; ≥ 94%), and DiBAC4(3) (44977; ≥ 98%) were purchased from Sigma-Aldrich (Shanghai, China).

    Techniques: Membrane, Permeability, Laser-Scanning Microscopy, Fluorescence, Activity Assay, Two Tailed Test, Concentration Assay