cbs 296 29  (ATCC)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

doi: 10.1155/2016/2386068

Figure Lengend Snippet: HK-2 cells injury after 4 hours of hypoxia followed by 2 hours of reoxygenation under high glucose stimulation. Effects of TXNIP blockage on cell viability assessed by CCK-8 (a); lactate dehydrogenase (LDH) release (b). The data in (a-b) are means ± SE ( n = 6). # P < 0.05 versus NG group; ☆ P < 0.05 versus NH/R group; & P < 0.05 versus HH/R-scrambled siRNA group; NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG condition; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG condition. HH/R-RES: HH/R pretreated by RES (50 μ M) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R.

Article Snippet: VDUP-1 (TXNIP) siRNA Plasmid (sc-44943) and control siRNA Plasmid were from Santa Cruz Biotechnology.

Techniques: CCK-8 Assay, Incubation, Transfection

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

doi: 10.1155/2016/2386068

Figure Lengend Snippet: Western blot analysis of TXNIP and NLRP3 protein expression in cultured HK-2 cells treated by normal glucose (5.5 mM), high glucose (30 mM), and NG + mannitol, respectively, for 72 hours, then following 4 hours of hypoxia and 2 hours of reoxygenation in HK-2 cells under high glucose stimulation with or without TXNIP siRNA and RES treatment, respectively. Representative blots (a) and quantitative analysis of Western blots for TXNIP (c) and NLRP3 (d), activity of caspase-1 (e), level of IL-1 β (f), and Western blot of TXNIP gene knockdown in HK-2 cells (b). The data in (c–f) are means ± SE ( n = 5). # P < 0.05 versus NG group; ☆ P < 0.05 versus NH/R group; & P < 0.05 versus HH/R-scrambled siRNA group.

Article Snippet: VDUP-1 (TXNIP) siRNA Plasmid (sc-44943) and control siRNA Plasmid were from Santa Cruz Biotechnology.

Techniques: Western Blot, Expressing, Cell Culture, Activity Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

doi: 10.1155/2016/2386068

Figure Lengend Snippet: Immunofluorescence staining of TXNIP (a) and NLRP3 (b). NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG conditions; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG conditions. HH/R-RES: HH/R pretreated by RES (50 μ M) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R.

Article Snippet: VDUP-1 (TXNIP) siRNA Plasmid (sc-44943) and control siRNA Plasmid were from Santa Cruz Biotechnology.

Techniques: Immunofluorescence, Staining, Incubation, Transfection

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

doi: 10.1155/2016/2386068

Figure Lengend Snippet: Effect of TXNIP inhibition on the HH/R-induced apoptosis and oxidative stress in HK-2 cells. Apoptotic cells were defined as the cells in the right two quadrants of each plot and the percentages were determined by flow cytometry (a, b); intracellular ROS was detected by flow cytometry (c); SOD (d) and MDA (e) were detected by microplate reader. The data in (b–e) are means ± SE ( n = 5). # P < 0.05 versus NG group; ☆ P < 0.05 versus NH/R group; & P < 0.05 versus HH/R-scrambled siRNA group. NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG condition; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG condition. HH/R-RES: HH/R pretreated by RES (50 μ M) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R.

Article Snippet: VDUP-1 (TXNIP) siRNA Plasmid (sc-44943) and control siRNA Plasmid were from Santa Cruz Biotechnology.

Techniques: Inhibition, Flow Cytometry, Incubation, Transfection

Journal: Oncology Reports

Article Title: 1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol ameliorates EGF-induced MMP-9 expression by promoting receptor desensitization in MDA-MB-231 cells

doi: 10.3892/or.2020.7599

Figure Lengend Snippet: TXNIP induction by PLAG treatment. (A) TXNIP mRNA and protein expression was analyzed by RT-PCR and western blotting, respectively. (B) EGFR-bound TXNIP was confirmed by western blotting via co-immunoprecipitation in PLAG-only treated cells. (C) TXNIP knockdown was confirmed by RT-PCR. (D) After treatment with PLAG and EGF, EGFR internalization was analyzed by flow cytometry. (E-G) PLAG did not reduce EGF-induced cell migration and invasion in TXNIP-silenced cells. Invasive and migrating cells were counted in the assay at ×200. (H) MMP-9 expression was not modified in the si-TXNIP treated cells. MMP-9 expression was analyzed by RT-PCR 6 h, and by western blotting 24 h, after stimulation. Statistical significance was determined by ANOVA (Tukey's test). **P<0.01 and ***P<0.005, compared with the untreated group; ### P<0.005, compared with the EGF group. N.S., not significant; PLAG, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; MMP-9, matrix metalloproteinase 9; TXNIP, thioredoxin-interacting protein; si-Con, scrambled siRNA transfected MDA-MB-231 breast cancer cells; si-TXNIP, TXNIP siRNA transfected MDA-MB-231 breast cancer cells.

Article Snippet: TXNIP siRNA (cat. no. sc-270490) was purchased from Santa Cruz Biotechnology, Inc.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunoprecipitation, Flow Cytometry, Migration, Modification, Transfection