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amp r 20 449 end bf3242c bacteroides  (ATCC)


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    ATCC amp r 20 449 end bf3242c bacteroides
    Amp R 20 449 End Bf3242c Bacteroides, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    An atlas of immune cells in patients <t>with</t> <t>UCEC.</t> (A) , (B) A UMAP projection of all cells from one patient with UCEC and one matched healthy control. Different colors indicate cell clusters. (C) Box plot highlights the contribution of two groups to each cell cluster. (D) Expression of marker genes of each cell type defined in (A) . (E, F) scRNA-seq analysis of <t>CAPG</t> expression in 26 cell subsets. (G) Average expression level and percent expression of CAPG in tumor and control groups. UMAP: Uniform Manifold Approximation and Projection; scRNA-seq: single-cell RNA-sequencing.
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    An atlas of immune cells in patients with UCEC. (A) , (B) A UMAP projection of all cells from one patient with UCEC and one matched healthy control. Different colors indicate cell clusters. (C) Box plot highlights the contribution of two groups to each cell cluster. (D) Expression of marker genes of each cell type defined in (A) . (E, F) scRNA-seq analysis of CAPG expression in 26 cell subsets. (G) Average expression level and percent expression of CAPG in tumor and control groups. UMAP: Uniform Manifold Approximation and Projection; scRNA-seq: single-cell RNA-sequencing.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: An atlas of immune cells in patients with UCEC. (A) , (B) A UMAP projection of all cells from one patient with UCEC and one matched healthy control. Different colors indicate cell clusters. (C) Box plot highlights the contribution of two groups to each cell cluster. (D) Expression of marker genes of each cell type defined in (A) . (E, F) scRNA-seq analysis of CAPG expression in 26 cell subsets. (G) Average expression level and percent expression of CAPG in tumor and control groups. UMAP: Uniform Manifold Approximation and Projection; scRNA-seq: single-cell RNA-sequencing.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Control, Expressing, Marker, RNA Sequencing Assay

    Expression levels of CAPG and its correlation with UCEC prognosis. (A) Human CAPG expression levels in different cancer types from TCGA data in TIMER. (B-G) CAPG expression levels in subgroups of UCEC patients by the UALCAN database. (H) Immunohistochemical images of CAPG protein in endometrial carcinoma and adjacent tissues. (I) Correlation between CAPG gene expression and UCEC prognosis. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Expression levels of CAPG and its correlation with UCEC prognosis. (A) Human CAPG expression levels in different cancer types from TCGA data in TIMER. (B-G) CAPG expression levels in subgroups of UCEC patients by the UALCAN database. (H) Immunohistochemical images of CAPG protein in endometrial carcinoma and adjacent tissues. (I) Correlation between CAPG gene expression and UCEC prognosis. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Expressing, Immunohistochemical staining

    Univariate and multivariate analyses of the correlation between CAPG expression and overall survival rate among UCEC patients.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Univariate and multivariate analyses of the correlation between CAPG expression and overall survival rate among UCEC patients.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Expressing

    DNA methylation analysis of CAPG gene. (A) Methylation levels of CAPG promoters in UCEC and control groups. CAPG promoter methylation levels at different stages (B) , grades (C) , and TP53 mutant (D) of UCEC. (E) Distribution of CpG island in the CAPG promoter region. Prediction of transcription factors binding to each CpG island of CAPG: (F) SOX10, (G) KLF1, (H) THAP1.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: DNA methylation analysis of CAPG gene. (A) Methylation levels of CAPG promoters in UCEC and control groups. CAPG promoter methylation levels at different stages (B) , grades (C) , and TP53 mutant (D) of UCEC. (E) Distribution of CpG island in the CAPG promoter region. Prediction of transcription factors binding to each CpG island of CAPG: (F) SOX10, (G) KLF1, (H) THAP1.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: DNA Methylation Assay, Methylation, Control, Mutagenesis, Binding Assay

    Analysis of immune cell infiltration in UCEC. (A) GO and KEGG analyses of CAPG in UCEC. (B) Estimated proportion of each-type immune cell in tumor and normal endometrium tissues using CIBERSORT algorithm. (C) Scatterplot of correlation between CAPG expression in UCEC and immune infiltration level. (D) Relative expression levels of immune-related genes IL8, TNF, and TLR4 in Ishikawa cells treated with si-CAPG. (E) Comparison of ssGSEA scores of the 28-types immune cells between high- and low-risk group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significant (P > 0.05).

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Analysis of immune cell infiltration in UCEC. (A) GO and KEGG analyses of CAPG in UCEC. (B) Estimated proportion of each-type immune cell in tumor and normal endometrium tissues using CIBERSORT algorithm. (C) Scatterplot of correlation between CAPG expression in UCEC and immune infiltration level. (D) Relative expression levels of immune-related genes IL8, TNF, and TLR4 in Ishikawa cells treated with si-CAPG. (E) Comparison of ssGSEA scores of the 28-types immune cells between high- and low-risk group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significant (P > 0.05).

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Expressing, Comparison

    Correlations between CAPG and gene markers of immune cells in UCEC by TIMER (None and Purity) or by Pearson analysis (Tum our).

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Correlations between CAPG and gene markers of immune cells in UCEC by TIMER (None and Purity) or by Pearson analysis (Tum our).

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Marker

    Ferroptosis analysis in UCEC. (A) Ferroptosis scores in scRNA-seq analysis. (B) Correlation between CAPG expression level and ferroptosis marker set. Left: negative correlation; Right: positive correlation. (C, D) Metabolic scores of ferroptosis-related metabolic pathways in UCEC immune cells. (E) Glutathione metabolism level in tumor and control groups.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Ferroptosis analysis in UCEC. (A) Ferroptosis scores in scRNA-seq analysis. (B) Correlation between CAPG expression level and ferroptosis marker set. Left: negative correlation; Right: positive correlation. (C, D) Metabolic scores of ferroptosis-related metabolic pathways in UCEC immune cells. (E) Glutathione metabolism level in tumor and control groups.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Expressing, Marker, Control

    Potential physiological function of CAPG on Ishikawa cells, si- CAPG -induced ferroptosis, and the expression and co-localization of CAPG and GPX4 in Ishikawa cells. (A) EdU staining demonstrated that si- CAPG significantly inhibited the proliferation of Ishikawa cells. Scale bar = 40μm. (B) Invasion of Ishikawa cells treated with si- CAPG was evaluated by transwell assay. Scale bar = 200μm. (C) Representative images of each group of Ishikawa cells treated by NC, si- CAPG , erastin and si- CAPG +Fer-1 and stained by FerroOrange were captured. Scale bar = 40μm. (D) Representative fluorescence images of nuclear and cytoplasmic localization of CAPG (red) and GPX4 (green) in UCEC cell line by immunofluorescence. Nucleus was stained with DAPI (blue). Scale bar = 40μm. ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significant ( P > 0.05). DAPI: 4′,6-diamidino-2-phenylindole.

    Journal: Frontiers in Endocrinology

    Article Title: Identification of CAPG as a potential prognostic biomarker associated with immune cell infiltration and ferroptosis in uterine corpus endometrial carcinoma

    doi: 10.3389/fendo.2024.1452219

    Figure Lengend Snippet: Potential physiological function of CAPG on Ishikawa cells, si- CAPG -induced ferroptosis, and the expression and co-localization of CAPG and GPX4 in Ishikawa cells. (A) EdU staining demonstrated that si- CAPG significantly inhibited the proliferation of Ishikawa cells. Scale bar = 40μm. (B) Invasion of Ishikawa cells treated with si- CAPG was evaluated by transwell assay. Scale bar = 200μm. (C) Representative images of each group of Ishikawa cells treated by NC, si- CAPG , erastin and si- CAPG +Fer-1 and stained by FerroOrange were captured. Scale bar = 40μm. (D) Representative fluorescence images of nuclear and cytoplasmic localization of CAPG (red) and GPX4 (green) in UCEC cell line by immunofluorescence. Nucleus was stained with DAPI (blue). Scale bar = 40μm. ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significant ( P > 0.05). DAPI: 4′,6-diamidino-2-phenylindole.

    Article Snippet: To elucidate the physiological function of CAPG on Human UCEC cell line Ishikawa cells, we designed and synthesized CAPG knockdown sequences from Santa Cruz Biotechnology (code number: sc-44920).

    Techniques: Expressing, Staining, Transwell Assay, Fluorescence, Immunofluorescence