Journal: Cell reports

Article Title: Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation

doi: 10.1016/j.celrep.2021.109122

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: pFA6a-link-yoEGFP-Kan , Addgene , Cat #: 44900.

Techniques: Binding Assay, Recombinant, Staining, Western Blot, Stripping, Modification, Clone Assay, Stable Transfection, Expressing, Plasmid Preparation, Software

Journal: Current biology : CB

Article Title: Critical roles of a RhoGEF-anillin module in septin architectural remodeling during cytokinesis

doi: 10.1016/j.cub.2020.02.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: KG, Karlsruhe, Germany Cat #: 5447.1 Sodium Chloride Sigma, Darmstadt, Germany Cat #: 31434 Zymolyase 100T Amsbio, Oxfordshire, UK Cat#: 120493–1 Glutaraldehyde Polyscience, Pennsylvania, USA Cat#: 01909–10 Anti-GFP antibody Abcam, Cambridge, USA Cat#: ab5450 18 nm Colloidal Gold AffiniPure Donkey Anti-Goat IgG (H+L) Jackson ImmunoResearch, Pennsylvania, USA Cat#: 705-215-147 Tannic Acid Mallinckrodt, Kentucky, USA Cat#:1764 Uranyl Acetate Structure Probe, Pennsylvania, USA Cat#:02624 Experimental Models: Organisms/Strains Saccharomyces cerevisiae strains, see Table S1 This paper N/A Oligonucleotides Primers, see Table S2 This paper N/A Recombinant DNA Plasmid: YIp128-CDC3-GFP [ 48 ] N/A Plasmid: YIp128-CDC3-mCherry [ 61 ] N/A Plasmid: pFA6a-TRP1 Addgene Cat #: 41595 Plasmid: pAG25 Addgene Cat #: 35121 Plasmid: pFA6a-URA3-KanMX6 John Pringle N/A Plasmid: pHIS3p: mRuby2-Tub1+3’UTR::HIS3 [ 62 ] N/A Plasmid: pHIS3p: mRuby2-Tub1+3’UTR::HPH [ 62 ] N/A Plasmid: pFA6a- link-yoEGFP-Sp HIS5 Addgene Cat #: 44836 Plasmid: pFA6a-link-yoEGFP-KAN Addgene Cat #: 44900 Software and Algorithms MetaMorph version 7.8.10.0 Molecular Devices https://www.moleculardevices.com/ Fiji [ 63 ] https://imagej.net/Fiji/ NIH ImageJ (1.51j) NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Plasmid Preparation, Software

Journal: Frontiers in Immunology

Article Title: 1-Palmitoyl-2-Linoleoyl-3-Acetyl-rac-Glycerol (PLAG) Rapidly Resolves LPS-Induced Acute Lung Injury Through the Effective Control of Neutrophil Recruitment

doi: 10.3389/fimmu.2019.02177

Figure Lengend Snippet: PLAG has an effect on accelerated TLR4 endocytosis via LPL and GPI-HBP1 dependent pathway. Raw264.7 cells were transfected with LPL, GPI-HBP1, caveolin-1, or clathrin siRNA, respectively. After 24 h, LPL, GPI-HBP1, caveolin-1, or clathrin levels were assessed by western blot (A) and cells were incubated with 100 ug/ml of PLAG or DMSO (as solvent control) for 1 h. LPS was treated with 100 ng/ml for 15, 30, 60, 120 min, cells were fixed, permeabilized, and stained using rat anti-TLR4/MD2 antibody and Alexa488 conjugated anti-rat IgG as secondary antibody. Cells were analyzed confocal analysis (B–F) . β-actin was used as a control. All data shown represent one experiment performed in triplicate.

Article Snippet: Specific siRNA targeting TRIF (sc-154266), TRAM (sc-44748), TIRAP (sc-44740), Myd88 (sc-35987), lipoprotein lipase (LPL) (sc-44900), glycosylphosphatidylinositol high density binding protein 1 (GPI-HBP1) (sc-145686), clathrin (sc-35067), and caveolin-1 (sc-29241) were purchased from Santa Cruz Biotechnology.

Techniques: Transfection, Western Blot, Incubation, Staining

Journal: Frontiers in Immunology

Article Title: 1-Palmitoyl-2-Linoleoyl-3-Acetyl-rac-Glycerol (PLAG) Rapidly Resolves LPS-Induced Acute Lung Injury Through the Effective Control of Neutrophil Recruitment

doi: 10.3389/fimmu.2019.02177

Figure Lengend Snippet: PLAG functions via micelle formation. Raw264.7 cells were transfected with siRNA targeting LPL or GPI-HBP1, and after 24 h, expression of target genes was assessed by RT-PCR (A) . Transfected cells were incubated with 10 or 100 μg/ml of PLAG or DMSO (as solvent control) for 1 h and then treated with 100 ng/ml LPS for 16 h. Culture supernatants were assayed by ELISA to measure secreted levels of MIP-2, IFN-β, IL-1β, and TNF (B) . Raw264.7 cells were transfected with siRNA targeting clathrin or caveolin-1, and after 24 h, expression of target genes was assessed by RT-PCR (C) . Transfected cells were then treated with 10 or 100 μg/ml of PLAG or DMSO (as solvent control) for 1 h and stimulated with 100 ng/ml LPS for 16 h. Culture supernatants were analyzed by ELISA assay to measure secreted levels of MIP-2, IFN-β, IL-1β, and TNF (D) . Data represent one experiment performed in triplicate. * indicates p < 0.05.

Article Snippet: Specific siRNA targeting TRIF (sc-154266), TRAM (sc-44748), TIRAP (sc-44740), Myd88 (sc-35987), lipoprotein lipase (LPL) (sc-44900), glycosylphosphatidylinositol high density binding protein 1 (GPI-HBP1) (sc-145686), clathrin (sc-35067), and caveolin-1 (sc-29241) were purchased from Santa Cruz Biotechnology.

Techniques: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Enzyme-linked Immunosorbent Assay