Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in a mouse cell line using 4 different commercial anti-mouse TF antibodies. Proteins in cell lysates (10 μg) from the mouse pancreatic cancer cell lines KPC2 wild-type (WT) and KPC2 TF knockout (KO) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a membrane. TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody. These anti-TF antibodies were detected using species-specific secondary antibodies . β-Actin was used as a loading control and detected with an anti–β-actin antibody . The binding of the anti–β-actin antibody was detected with a horse anti-mouse immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the blots were exposed for 1 second for both TF and β-actin. All blots were visualized using the iBright FL1000 imaging system. kDa, kilodalton.

Article Snippet: TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody.

Techniques: Knock-Out, Polyacrylamide Gel Electrophoresis, Membrane, Control, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) using the Santa Cruz antibody. Proteins in 2 sets of lysates from mouse brain (B), heart (H), lung (Lu), and spleen (S; 40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a membrane. The membrane was then cut into halves. One-half was incubated overnight with the Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody. The other half was incubated in 5% bovine serum albumin in Tris-buffered saline with 0.1% Tween. Both membranes were then incubated with a horse anti-mouse immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the blots were exposed for 10 seconds (TF) and 15 seconds (α-actinin). All blots were visualized using the iBright FL1000 imaging system. kDa, kilodalton.

Article Snippet: TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody.

Techniques: Polyacrylamide Gel Electrophoresis, Membrane, Incubation, Saline, Control, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the R&D Systems antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody. The binding of the R&D Systems anti-TF antibody was detected with a rabbit anti-goat immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for 1 second. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody.

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the Abcam antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the Abcam ab189483 rabbit anti-mouse TF monoclonal antibody. The binding of the Abcam anti-TF antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for (A) 10, (B, top panel) 1, or (B, middle panel) 15 seconds. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody.

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the Cell Signaling Technology antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the Cell Signaling Technology 44861 rabbit anti-mouse TF monoclonal antibody. The binding of the Cell Signaling Technology anti-TF antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for (A) 2, (B, top panel) 1, or (B, middle panel) 15 seconds. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody.

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging