nocardia tenerifensis dsm 44704t  (ATCC)

Journal: Cell reports

Article Title: Hepatocyte Period 1 dictates oxidative substrate selection independent of the core circadian clock

doi: 10.1016/j.celrep.2024.114865

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: si Esrrg , Santa Cruz Biotechnology , Cat#sc-44705.

Techniques: Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, In Vivo, Sequencing, Software

Journal: Molecular human reproduction

Article Title: Trophoblast syncytialisation necessitates mitochondrial function through estrogen-related receptor-γ activation.

doi: 10.1093/molehr/gau102

Figure Lengend Snippet: Figure 3 Effect of estrogen-related receptor gamma (ERRg) on biochemical CT differentiation. CTs were transfected with siERRg1 or with siNS as a control, or treated with or without DY131, oligomycin or DY131 plus oligomycin. Total RNA were extracted and supernatants cells were collected 24 or 48 h after DY131 treatment and ERRg1 invalidation, respectively. (A) hCG data are the medians (interquartile range) of eight separate experiments for siNS and siERRg1. The controlmean valuewas2293.08+486.22 IU/g protein. *P ≤0.05; Wilcoxon test.(B) hCG dataarethe medians (interquartile range) of 12 separate experiments for control and DY131. **P ≤0.005; Wilcoxon test. (C) hCG data are the medians (interquartile range) of 16 separate experiments for control and oligomycin, and 3 separate experiments for oligomycin+DY131. ***P ≤0.001, ns: non-significant; Wilcoxon test. (D) Leptin mRNA expression was quantified by RT–PCR. Results are the medians (interquartile range) of six separate experiments for siNS and siERRg1. *P ≤0.05, Wilcoxon test. (E) Leptin mRNA expression was quantified by RT–PCR. Results are the medians (interquartile range) of six separate experiments for control and DY131. *P ≤0.05, Wilcoxon test.

Article Snippet: Cells were then cultured for an additional 48 h. siRNA knockdown of ERRg After 12 h of culture, the CTs were transfected with small interfering ERRg1 (siERRg1) (10 nM, sc-44704, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Transfection, Control, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Molecular human reproduction

Article Title: Trophoblast syncytialisation necessitates mitochondrial function through estrogen-related receptor-γ activation.

doi: 10.1093/molehr/gau102

Figure Lengend Snippet: Figure 4 Effect of ERRg on morphological CT differentiation. CTs were transfected with siERRg1 or with siNS as a control, or treated with or without DY131, oligomycin. Cells were fixed and total RNA were extracted 48 h after DY131 treatment and ERRg1 invalidation, respectively. (A) Syncytin-2 mRNA expression was quantified by RT–PCR. Results are the medians (interquartile range) of 10 separate experiments. **P ≤0.005; Wilcoxon test. (B) Polyploidy ratio quantified after counting at least 50 cells in each groups from six representative separate experiments. Results are expressed as medians (interquartile range). The polyploidy ratio in control CTs was 0.089+ 0.009. *P ≤0.05, ***P ≤0.001; Wilcoxon test. (C) CTs cultivated in labtechweretransfectedwithsiERRg1orwithsiNSasacontrol,ortreatedwithorwithoutDY131.Cellswerefixed48 hafterwardsandE-cadherinstaining was performed. (a) siNS, (b) siERRg1, (c) non-treated cells, (d) DY131 treated cells and (e) negative control using mouse non-specific serum instead of primary antibody. Magnification ×70. The figure shows one representative data from six separate experiments.

Article Snippet: Cells were then cultured for an additional 48 h. siRNA knockdown of ERRg After 12 h of culture, the CTs were transfected with small interfering ERRg1 (siERRg1) (10 nM, sc-44704, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Transfection, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control

Journal: Molecular human reproduction

Article Title: Trophoblast syncytialisation necessitates mitochondrial function through estrogen-related receptor-γ activation.

doi: 10.1093/molehr/gau102

Figure Lengend Snippet: Figure 5 Effect of ERRg on mitochondrial biogenesis during CT differentiation. CTs were transfected with siERRg1 or with siNS as a control. Mitochon- drial DNA content and mass were measured 12 h or 48 h after transfection. CTs: 12 h transfected control cells (siNS 12 h), syncytiotrophoblast (ST): 48 h transfectedcontrolcells(siNS 48 h), and 48 h ERRg1invalidated cells (siERRg148 h). (A) Mitochondrial DNAdata arethe medians (interquartile range)of 4 separate experiments for siNS 12 h, 16 separate experiments for siNS 48 h and 8 separate experiments for siERRg1 48 h. *P ≤0.05; ns: non-significant; Wilcoxon test. (B) Mitochondrial mass analysis by confocal microscopy. (a) siNS 12 h, (b) siNS 48 h, (c) siERRg1 48 h, and (d) negative control: CTs processedwithoutMitotracker.MitochondriamasswasanalysedbystainingwithMitotrackerRed.Magnification ×63.Thefigureshowsonerepresentative data from six separate experiments. (C) Quantification of mitochondrial mass: the area of mitochondrial staining per cell was determined using the Image J software available at http://imagej.nih.gov/ij. Results are the medians (interquartile range) of six separate experiments. **P ≤0.005, *P ≤0.05; Wilcoxon test.

Article Snippet: Cells were then cultured for an additional 48 h. siRNA knockdown of ERRg After 12 h of culture, the CTs were transfected with small interfering ERRg1 (siERRg1) (10 nM, sc-44704, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Transfection, Control, Confocal Microscopy, Negative Control, Staining, Software

Journal: Molecular human reproduction

Article Title: Trophoblast syncytialisation necessitates mitochondrial function through estrogen-related receptor-γ activation.

doi: 10.1093/molehr/gau102

Figure Lengend Snippet: Figure 6 Effect of ERRg on mitochondrial activity during CT differentiation. CTs were transfected with siERRg1 or with siNS as a control. Lactate pro- ductionandATPquantificationweremeasuredafter12and48 hoftransfection.CTs:12 htransfectedcontrolcells(siNS12 h),ST:48 htransfectedcontrol cells (siNS 48 h) and 48 h ERRg1 invalidated cells (siERRg1 48 h). (A) ATP data are the medians (interquartile range) of five separate experiments. Control values were 0.0073+ 0.0001 and 0.0067+ 0.0008 mM ATP for siNS 12 h and siNS 48 h, respectively. *P ≤0.05; ns: non-significant; Wilcoxon test. (B) Lactate data are the medians (interquartile range) of six separate experiments. Control values were 0.97+0.13 and 1.33 +0.2 mmol/g protein for siNS 12 h and siNS 48 h, respectively. *P ≤0.05; ns: non-significant; Wilcoxon test.

Article Snippet: Cells were then cultured for an additional 48 h. siRNA knockdown of ERRg After 12 h of culture, the CTs were transfected with small interfering ERRg1 (siERRg1) (10 nM, sc-44704, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Activity Assay, Transfection, Control