ice r  (ATCC)


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    ATCC ice r
    Ice R, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ice r  (ATCC)


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    ATCC ice r
    Ice R, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ice r/product/ATCC
    Average 90 stars, based on 1 article reviews
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    ice r  (ATCC)


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    ATCC ice r
    (A) Diagrammatic depictions of the conserved insertion target (GA) at 11th codon from the 3′ end of heterologous rumA genes. The differently colored rumA structural genes and gene segments represent the different homologs that share a deduced range of about 36% to 74% amino acid sequence identity. The conserved chromosomal location of the GBS 3′ end in relation to the hemolysin operon cylX gene is indicated (and is also indicated in panels B, C, and D). Genomes containing insertion elements ( vanG - vanG- like and <t>ICE-r/ICE-r-like)</t> are indicated (see panels B to H). The rumA5 ′ and rumA3 ′ segments (see panels B to H) shared sequence identity with their overlaps in the intact rumA gene segments from the corresponding GenBank genome sequences indicated in panel A. (B) The ICE-r element found <t>in</t> <t>ST22</t> GBS strains present in the NCBI wgs database. ILR1 and IRR1 indicate imperfect inverted repeats associated with ICE-r. The direct repeats of the dinucleotide target in rumA (GA) are also indicated (R1 and R2). The near-identical ICE-r derivative of the ST22 GBS6 study strain differs from these other isolates by a nonhomologous 12.9-kb insertion (not shown). In panels B to H, inverted repeats are underlined on both sides of 1 or 2 elements (on one side only of the ICE-r derivative shown in panel E). The 8-bp and 3-bp sequences that are shared between vanG and ICE-r element termini are indicated in green. (C and D) The tandem vanG and ICE-r insertions in strains GBS-NY and GBS-NM with the vanG element imperfect inverted repeats (ILR2 and IRR2) and ICE-r inverted repeats (ILR1 and IRR2). Base substitutions in the left repeat (LR) and right repeat (RR) relative to vanG-1 are shown in red (D). (E) The vanG-1 insertion within the S. anginosus rumA gene is followed by an ICE-r derivative that shares high intermittent homology with ICE-r from GBS and S. equisimilis ATCC 12394 (see panels B, C, D, and F). Although the rumA 5′ segment was identical to its counterpart in S. anginosus F0211 (see panel A), a corresponding ICE-r IRR1 and rumA 3′ segment was absent. Base changes in the unmatched ICE-r derivative inverted left repeat relative to ICE-r (panels B to D) are shown in red. (F) ICE-r element nearly perfectly conserved with GBS-NY inserted within S. dysgalactiae subsp. equisimilis rumA gene. (G) Diagram of previously published information ( , ) describing vanG insertions in two different E. faecalis strains. The slanted vertical lines for the E. faecalis vanG elements indicate that the lengths are presently unknown. (H) The vanG -like insertion element in R. intestinalis XB6B3, which has high homology with vanG-1 (see <xref ref-type=Fig. S1 in the supplemental material). " width="250" height="auto" />
    Ice R, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ice r/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ice r - by Bioz Stars, 2024-04
    90/100 stars

    Images

    1) Product Images from "vanG Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to Streptococcus agalactiae and Streptococcus anginosus"

    Article Title: vanG Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to Streptococcus agalactiae and Streptococcus anginosus

    Journal: mBio

    doi: 10.1128/mBio.01386-14

    (A) Diagrammatic depictions of the conserved insertion target (GA) at 11th codon from the 3′ end of heterologous rumA genes. The differently colored rumA structural genes and gene segments represent the different homologs that share a deduced range of about 36% to 74% amino acid sequence identity. The conserved chromosomal location of the GBS 3′ end in relation to the hemolysin operon cylX gene is indicated (and is also indicated in panels B, C, and D). Genomes containing insertion elements ( vanG - vanG- like and ICE-r/ICE-r-like) are indicated (see panels B to H). The rumA5 ′ and rumA3 ′ segments (see panels B to H) shared sequence identity with their overlaps in the intact rumA gene segments from the corresponding GenBank genome sequences indicated in panel A. (B) The ICE-r element found in ST22 GBS strains present in the NCBI wgs database. ILR1 and IRR1 indicate imperfect inverted repeats associated with ICE-r. The direct repeats of the dinucleotide target in rumA (GA) are also indicated (R1 and R2). The near-identical ICE-r derivative of the ST22 GBS6 study strain differs from these other isolates by a nonhomologous 12.9-kb insertion (not shown). In panels B to H, inverted repeats are underlined on both sides of 1 or 2 elements (on one side only of the ICE-r derivative shown in panel E). The 8-bp and 3-bp sequences that are shared between vanG and ICE-r element termini are indicated in green. (C and D) The tandem vanG and ICE-r insertions in strains GBS-NY and GBS-NM with the vanG element imperfect inverted repeats (ILR2 and IRR2) and ICE-r inverted repeats (ILR1 and IRR2). Base substitutions in the left repeat (LR) and right repeat (RR) relative to vanG-1 are shown in red (D). (E) The vanG-1 insertion within the S. anginosus rumA gene is followed by an ICE-r derivative that shares high intermittent homology with ICE-r from GBS and S. equisimilis ATCC 12394 (see panels B, C, D, and F). Although the rumA 5′ segment was identical to its counterpart in S. anginosus F0211 (see panel A), a corresponding ICE-r IRR1 and rumA 3′ segment was absent. Base changes in the unmatched ICE-r derivative inverted left repeat relative to ICE-r (panels B to D) are shown in red. (F) ICE-r element nearly perfectly conserved with GBS-NY inserted within S. dysgalactiae subsp. equisimilis rumA gene. (G) Diagram of previously published information ( , ) describing vanG insertions in two different E. faecalis strains. The slanted vertical lines for the E. faecalis vanG elements indicate that the lengths are presently unknown. (H) The vanG -like insertion element in R. intestinalis XB6B3, which has high homology with vanG-1 (see <xref ref-type=Fig. S1 in the supplemental material). " title="... insertion elements ( vanG - vanG- like and ICE-r/ICE-r-like) are indicated (see panels B to H). The ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: (A) Diagrammatic depictions of the conserved insertion target (GA) at 11th codon from the 3′ end of heterologous rumA genes. The differently colored rumA structural genes and gene segments represent the different homologs that share a deduced range of about 36% to 74% amino acid sequence identity. The conserved chromosomal location of the GBS 3′ end in relation to the hemolysin operon cylX gene is indicated (and is also indicated in panels B, C, and D). Genomes containing insertion elements ( vanG - vanG- like and ICE-r/ICE-r-like) are indicated (see panels B to H). The rumA5 ′ and rumA3 ′ segments (see panels B to H) shared sequence identity with their overlaps in the intact rumA gene segments from the corresponding GenBank genome sequences indicated in panel A. (B) The ICE-r element found in ST22 GBS strains present in the NCBI wgs database. ILR1 and IRR1 indicate imperfect inverted repeats associated with ICE-r. The direct repeats of the dinucleotide target in rumA (GA) are also indicated (R1 and R2). The near-identical ICE-r derivative of the ST22 GBS6 study strain differs from these other isolates by a nonhomologous 12.9-kb insertion (not shown). In panels B to H, inverted repeats are underlined on both sides of 1 or 2 elements (on one side only of the ICE-r derivative shown in panel E). The 8-bp and 3-bp sequences that are shared between vanG and ICE-r element termini are indicated in green. (C and D) The tandem vanG and ICE-r insertions in strains GBS-NY and GBS-NM with the vanG element imperfect inverted repeats (ILR2 and IRR2) and ICE-r inverted repeats (ILR1 and IRR2). Base substitutions in the left repeat (LR) and right repeat (RR) relative to vanG-1 are shown in red (D). (E) The vanG-1 insertion within the S. anginosus rumA gene is followed by an ICE-r derivative that shares high intermittent homology with ICE-r from GBS and S. equisimilis ATCC 12394 (see panels B, C, D, and F). Although the rumA 5′ segment was identical to its counterpart in S. anginosus F0211 (see panel A), a corresponding ICE-r IRR1 and rumA 3′ segment was absent. Base changes in the unmatched ICE-r derivative inverted left repeat relative to ICE-r (panels B to D) are shown in red. (F) ICE-r element nearly perfectly conserved with GBS-NY inserted within S. dysgalactiae subsp. equisimilis rumA gene. (G) Diagram of previously published information ( , ) describing vanG insertions in two different E. faecalis strains. The slanted vertical lines for the E. faecalis vanG elements indicate that the lengths are presently unknown. (H) The vanG -like insertion element in R. intestinalis XB6B3, which has high homology with vanG-1 (see Fig. S1 in the supplemental material).

    Techniques Used: Sequencing

    (A) Deduced amino acid sequence homology between the indicated left ends of vanG - vanG -like elements (red arrows), ICE-r elements (green arrows), and rumA 3′ segments (black arrows) of different translational fusions (diagrammed at the top of the panel). The residues that are completely conserved among the 7-element left ends are blue. Residues conserved in 4 to 5 left elements are red. Residues conserved among 3 elements are green. (B) The RumA C-terminal 11 residues from different species that are often translationally fused to different serine recombinase genes (srg) are directly aligned, with those conserved among the various elements shown in red. (C) Deduced related ICE-like element 5′ residues in the S. agalactiae NCBI database found to be fused with the wild-type chromosomal rumA 5′ end (below the horizontal line). Residues conserved with S. agalactiae wild-type rumA 3′ 11 codons indicated at the top (uninterrupted by an insertion element) are shown in red. Isolate identifiers or GenBank accession numbers are provided. The approximate lengths of fusion elements inserted within rumA are indicated in parentheses, based on the distance of the wild-type rumA 11 codons from rumA base 1328 and the cylX gene, consistently situated about 7,525 to 7,650 bp downstream (strains COH1, 260/V, NEM316, GB201008-001, A909, 09mas018883, and GB00003), or based on the distance of the wild-type rumA 11 codons from cylX (strains ILRI005 and ILRI112). Other strains with no lengths shown were from contigs that did not include the entire insertion element.
    Figure Legend Snippet: (A) Deduced amino acid sequence homology between the indicated left ends of vanG - vanG -like elements (red arrows), ICE-r elements (green arrows), and rumA 3′ segments (black arrows) of different translational fusions (diagrammed at the top of the panel). The residues that are completely conserved among the 7-element left ends are blue. Residues conserved in 4 to 5 left elements are red. Residues conserved among 3 elements are green. (B) The RumA C-terminal 11 residues from different species that are often translationally fused to different serine recombinase genes (srg) are directly aligned, with those conserved among the various elements shown in red. (C) Deduced related ICE-like element 5′ residues in the S. agalactiae NCBI database found to be fused with the wild-type chromosomal rumA 5′ end (below the horizontal line). Residues conserved with S. agalactiae wild-type rumA 3′ 11 codons indicated at the top (uninterrupted by an insertion element) are shown in red. Isolate identifiers or GenBank accession numbers are provided. The approximate lengths of fusion elements inserted within rumA are indicated in parentheses, based on the distance of the wild-type rumA 11 codons from rumA base 1328 and the cylX gene, consistently situated about 7,525 to 7,650 bp downstream (strains COH1, 260/V, NEM316, GB201008-001, A909, 09mas018883, and GB00003), or based on the distance of the wild-type rumA 11 codons from cylX (strains ILRI005 and ILRI112). Other strains with no lengths shown were from contigs that did not include the entire insertion element.

    Techniques Used: Sequencing

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