dsm 44639  (ATCC)

Journal: Experimental and Therapeutic Medicine

Article Title: Role of microRNA-15a-5p/TNFAIP3-interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock

doi: 10.3892/etm.2020.9130

Figure Lengend Snippet: TNIP2 is a direct target of miR-15a-5p. (A) Interaction between miR-15a-5p and 3'UTR of TNIP2 was predicted using TargetScan. (B) Luciferase activity of a reporter containing a wild-type TNIP2 3'UTR or a mutant TNIP2 3' UTR are presented. TNIP2-MUT indicates the TNIP2 3' UTR with a mutation in the miR-15a-5p binding site. All data are presented as the mean ± SD of three independent experiments. ** P<0.01 vs. mimic control. miR, microRNA; UTR, untranslated region; THS, traumatic hemorrhagic shock; WT, wild type; TNIP2, TNFAIP3-interacting protein 2.

Article Snippet: Rats were intraperitoneally injected with inhibitor control (80 mg/kg/day; 5'-CAGUACUUUUGUGUAGUACAA-3'; Shanghai GenePharma Co., Ltd.), miR-15a-5p inhibitor (80 mg/kg/day; 5'-CACUGGUACAAGGGUUGGGAGA-3'; Shanghai GenePharma Co., Ltd.) or miR-15a-5p inhibitor (80 mg/kg/day) + TNIP2-siRNA (80 mg/kg/day; cat. no. sc-44638; Santa Cruz Biotechnology, Inc.) prior to surgery using in vivo transfection reagent (EntransterTM- in vivo ; Engreen Biosystem Co., Ltd.).

Techniques: Luciferase, Activity Assay, Mutagenesis, Binding Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Role of microRNA-15a-5p/TNFAIP3-interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock

doi: 10.3892/etm.2020.9130

Figure Lengend Snippet: Expression level of TNIP2 in patients with THS and rats. (A) mRNA and (B) protein level of TNIP2 in the blood of patients with THS was detected using RT-qPCR and western blotting (C1, C2: Healthy control; T1-T4: Patients with THS). (C) mRNA and (D) protein level of TNIP2 in the blood of THS rats was detected using RT-qPCR and western blotting. (E) mRNA and (F) protein level of TNIP2 in the lung tissues of THS rats was detected using RT-qPCR and western blotting. Data were expressed as mean ± SD. ** P<0.01 vs. control group. THS, traumatic hemorrhagic shock; TNIP2, TNFAIP3-interacting protein 2; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Rats were intraperitoneally injected with inhibitor control (80 mg/kg/day; 5'-CAGUACUUUUGUGUAGUACAA-3'; Shanghai GenePharma Co., Ltd.), miR-15a-5p inhibitor (80 mg/kg/day; 5'-CACUGGUACAAGGGUUGGGAGA-3'; Shanghai GenePharma Co., Ltd.) or miR-15a-5p inhibitor (80 mg/kg/day) + TNIP2-siRNA (80 mg/kg/day; cat. no. sc-44638; Santa Cruz Biotechnology, Inc.) prior to surgery using in vivo transfection reagent (EntransterTM- in vivo ; Engreen Biosystem Co., Ltd.).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Role of microRNA-15a-5p/TNFAIP3-interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock

doi: 10.3892/etm.2020.9130

Figure Lengend Snippet: Effect of miR-15a-5p on TNIP2 expression in THS rats. Rats were intraperitoneally injected with inhibitor control, or miR-15a-5p inhibitor prior to THS induction. After 24 h, the level of miR-15a-5p and TNIP2 in the blood and lung tissues of THS rats was determined. The level of miR-15a-5p in the (A) blood and (B) lung tissue of THS rats was detected using RT-qPCR. The TNIP2 mRNA level in the (C) blood and (D) lung tissue of THS rats was detected using RT-qPCR: The mRNA and protein level of TNIP2 in the blood of THS rats was detected using (E) RT-qPCR and (F) western blotting. The mRNA and protein level of TNIP2 in the lung tissues of THS rats was detected using (G) RT-qPCR and (H) western blotting. Data were expressed as mean ± SD. ** P<0.01 vs. control group. miR, microRNA; THS, traumatic hemorrhagic shock; TNIP2, TNFAIP3-interacting protein 2; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Rats were intraperitoneally injected with inhibitor control (80 mg/kg/day; 5'-CAGUACUUUUGUGUAGUACAA-3'; Shanghai GenePharma Co., Ltd.), miR-15a-5p inhibitor (80 mg/kg/day; 5'-CACUGGUACAAGGGUUGGGAGA-3'; Shanghai GenePharma Co., Ltd.) or miR-15a-5p inhibitor (80 mg/kg/day) + TNIP2-siRNA (80 mg/kg/day; cat. no. sc-44638; Santa Cruz Biotechnology, Inc.) prior to surgery using in vivo transfection reagent (EntransterTM- in vivo ; Engreen Biosystem Co., Ltd.).

Techniques: Expressing, Injection, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Role of microRNA-15a-5p/TNFAIP3-interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock

doi: 10.3892/etm.2020.9130

Figure Lengend Snippet: Effect of miR-15a-5p on pro-inflammatory factors production in THS rats. The levels of TNF-α in (A) BALF and (B) serum and the levels of IL-6 in (C) BALF and (D) serum in rats were detected using ELISA. Data are expressed as mean ± SD. ** P<0.01 vs. control group; ## P<0.01 vs. THS group; && P<0.01 vs. inhibitor group. miR, microRNA; THS, traumatic hemorrhagic shock; TNIP2, TNFAIP3-interacting protein 2; BALF, bronchoalveolar lavage fluid; IL, interleukin; TNF-α, tumor necrosis factor-α; siRNA, small interfering RNA.

Article Snippet: Rats were intraperitoneally injected with inhibitor control (80 mg/kg/day; 5'-CAGUACUUUUGUGUAGUACAA-3'; Shanghai GenePharma Co., Ltd.), miR-15a-5p inhibitor (80 mg/kg/day; 5'-CACUGGUACAAGGGUUGGGAGA-3'; Shanghai GenePharma Co., Ltd.) or miR-15a-5p inhibitor (80 mg/kg/day) + TNIP2-siRNA (80 mg/kg/day; cat. no. sc-44638; Santa Cruz Biotechnology, Inc.) prior to surgery using in vivo transfection reagent (EntransterTM- in vivo ; Engreen Biosystem Co., Ltd.).

Techniques: Enzyme-linked Immunosorbent Assay, Small Interfering RNA