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halalkalicoccus jeotgali b3 65 2809118 halalkalicoccus jeotgali b3 plasmid  (ATCC)


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    Structured Review

    ATCC halalkalicoccus jeotgali b3 65 2809118 halalkalicoccus jeotgali b3 plasmid
    Halalkalicoccus Jeotgali B3 65 2809118 Halalkalicoccus Jeotgali B3 Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halalkalicoccus jeotgali b3 65 2809118 halalkalicoccus jeotgali b3 plasmid/product/ATCC
    Average 90 stars, based on 1 article reviews
    halalkalicoccus jeotgali b3 65 2809118 halalkalicoccus jeotgali b3 plasmid - by Bioz Stars, 2025-02
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    Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses <t>Pax6</t> (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).
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    Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses <t>Pax6</t> (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).
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    Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses <t>Pax6</t> (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).
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    Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses <t>Pax6</t> (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).
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    Image Search Results


    Primer information for RT-PCR

    Journal: International Journal of Stem Cells

    Article Title: Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells

    doi: 10.15283/ijsc23037

    Figure Lengend Snippet: Primer information for RT-PCR

    Article Snippet: The primary antibodies used in this study were as follows: anti-NESTIN (1:500; BioLegend), anti-SOX2 (1:1,000; Sigma-Aldrich), anti-ZO1 (1:50; Thermo Fisher Scientific), anti-PAX6 (1:200; Novus Biologicals), anti-TUJ1 (1:2,000; BioLegend), anti-TH (1:2,000; Pel-Freez), anti-glial fibrillary acidic protein (GFAP, 1:2,000; DAKO), anti-OLIG2 antibody (1:1,000; Chemicon), anti-major histocompatibility complex (MHC) Class I+HLA A/B (1:200, MHC-I; Abcam), anti-MHC Class II (1:200, MHC-II; Abcam).

    Techniques:

    Pluripotent marker expressions of human pluripotent stem cells (hPSCs). (A) alkaline phosphatases staining is the result of using conventional dyes as substrates for measuring the activity of elevated alkaline phosphatase in hPSCs. The characteristics of the fluorescently labeled cells are observed in the general hPSCs morphologies. (B) Reverse transcription polymerase chain reaction analysis reveals the expression of three germ layer markers ( PAX6, LMO2, GSC ) in embryoid bodies. Scale bar=500 μm. hESC: human embryonic stem cell, hiPSC: human induced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.

    Journal: International Journal of Stem Cells

    Article Title: Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells

    doi: 10.15283/ijsc23037

    Figure Lengend Snippet: Pluripotent marker expressions of human pluripotent stem cells (hPSCs). (A) alkaline phosphatases staining is the result of using conventional dyes as substrates for measuring the activity of elevated alkaline phosphatase in hPSCs. The characteristics of the fluorescently labeled cells are observed in the general hPSCs morphologies. (B) Reverse transcription polymerase chain reaction analysis reveals the expression of three germ layer markers ( PAX6, LMO2, GSC ) in embryoid bodies. Scale bar=500 μm. hESC: human embryonic stem cell, hiPSC: human induced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.

    Article Snippet: The primary antibodies used in this study were as follows: anti-NESTIN (1:500; BioLegend), anti-SOX2 (1:1,000; Sigma-Aldrich), anti-ZO1 (1:50; Thermo Fisher Scientific), anti-PAX6 (1:200; Novus Biologicals), anti-TUJ1 (1:2,000; BioLegend), anti-TH (1:2,000; Pel-Freez), anti-glial fibrillary acidic protein (GFAP, 1:2,000; DAKO), anti-OLIG2 antibody (1:1,000; Chemicon), anti-major histocompatibility complex (MHC) Class I+HLA A/B (1:200, MHC-I; Abcam), anti-MHC Class II (1:200, MHC-II; Abcam).

    Techniques: Marker, Staining, Activity Assay, Labeling, Reverse Transcription, Polymerase Chain Reaction, Expressing

    Neural precursor cell specifications are similarly induced in various human pluripotent stem cells (hPSCs). (A) Neural rosettes were ide-ntified according to rosette markers such as PAX6 and ZO-1. (B) Repre-sentative images indicating the spherical neural mass markers NESTIN and SOX2. (C) After single cell dissocia-tion of neural precursor cells, the left panel also reveals the neural precu-rsor cell markers NESTIN and SOX2. The right panel presents the quantified data from the left panel. Scale bar=50 μm. hESC: human embryonic stem cell, hiPSC: human induced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.

    Journal: International Journal of Stem Cells

    Article Title: Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells

    doi: 10.15283/ijsc23037

    Figure Lengend Snippet: Neural precursor cell specifications are similarly induced in various human pluripotent stem cells (hPSCs). (A) Neural rosettes were ide-ntified according to rosette markers such as PAX6 and ZO-1. (B) Repre-sentative images indicating the spherical neural mass markers NESTIN and SOX2. (C) After single cell dissocia-tion of neural precursor cells, the left panel also reveals the neural precu-rsor cell markers NESTIN and SOX2. The right panel presents the quantified data from the left panel. Scale bar=50 μm. hESC: human embryonic stem cell, hiPSC: human induced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.

    Article Snippet: The primary antibodies used in this study were as follows: anti-NESTIN (1:500; BioLegend), anti-SOX2 (1:1,000; Sigma-Aldrich), anti-ZO1 (1:50; Thermo Fisher Scientific), anti-PAX6 (1:200; Novus Biologicals), anti-TUJ1 (1:2,000; BioLegend), anti-TH (1:2,000; Pel-Freez), anti-glial fibrillary acidic protein (GFAP, 1:2,000; DAKO), anti-OLIG2 antibody (1:1,000; Chemicon), anti-major histocompatibility complex (MHC) Class I+HLA A/B (1:200, MHC-I; Abcam), anti-MHC Class II (1:200, MHC-II; Abcam).

    Techniques:

    Primary antibody information.

    Journal: Molecular Vision

    Article Title: Embryonic stem cell–derived photoreceptor precursor cells differentiated by coculture with RPE cells

    doi:

    Figure Lengend Snippet: Primary antibody information.

    Article Snippet: PAX6 , 1:100 , Novus biologicals , , Littleton, CO.

    Techniques:

    Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses Pax6 (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).

    Journal: Scientific Reports

    Article Title: SMARCB1 deletion in atypical teratoid rhabdoid tumors results in human endogenous retrovirus K (HML-2) expression

    doi: 10.1038/s41598-021-92223-x

    Figure Lengend Snippet: Immunostaining and Immunohistochemistry of patient-derived AT/RT cells and tissues. ( a , b ) Two resected patient AT/RT demonstrate strong immunostaining with HML-2 Envelope (Env) monoclonal antibody (brown). ( c ) Cerebrum from a normal brain is negative when stained with the same antibody. ( d – l ) AT/RT cell lines express markers of multiple stages of differentiation; representative images are shown. ( d ) CHLA 02, a patient derived AT/RT cell line, expresses Pax6 (red), a marker for neuroectoderm, merged with immunostaining for HML-2 Env (green). ( e ) : This cell line also expresses Nestin (green), a marker of neural stem cells. ( f ) The same cell line expresses Oct4 (red), a pluripotency marker. ( g , h ) CHLA 04 cells, another AT/RT line, express Oct4 (green) and Nestin (red). ( i ) CHLA 05 cells also express Oct4 (green) and Nestin (red). ( j ) The same cell line also expresses βIII tubulin (red), a marker for neurons and HML-2 envelope (green). ( k ) CHLA 06 cells also express Nestin (red) and Oct4 (green). ( l ) The same cell line also expresses βIII tubulin (red) and HML-2 Env (green). Nuclei are stained with DAPI (blue). For IHC images, slides were scanned with a OptraScan Digital Pathology Scanner and processed in Adobe Photoshop for incorporation into the figure. For immunofluorescent images, an EVOS fluorescence microscope (AMG) was used. Images were acquired with the native software installed on the EVOS microscope and processed in Microsoft PowerPoint ( https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/evos-fl-cell-imaging-system.html ).

    Article Snippet: Primary antibodies: AntiCD98 antibody ab108300; anti-Tubulin β3 801213 (Bio-legend), Anti-Oct4 antibody AB3209 (Millipore sigma), Anti-nestin Mab 5326 (Millipore), Anti-Pax6 NBP1 51622 (Novus biologicals).

    Techniques: Immunostaining, Immunohistochemistry, Derivative Assay, Staining, Marker, Fluorescence, Microscopy, Software, Imaging