Journal: International Journal of Stem Cells
Article Title: Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells
doi: 10.15283/ijsc23037
Figure Lengend Snippet: Pluripotent marker expressions of human pluripotent stem cells (hPSCs). (A) alkaline phosphatases staining is the result of using conventional dyes as substrates for measuring the activity of elevated alkaline phosphatase in hPSCs. The characteristics of the fluorescently labeled cells are observed in the general hPSCs morphologies. (B) Reverse transcription polymerase chain reaction analysis reveals the expression of three germ layer markers ( PAX6, LMO2, GSC ) in embryoid bodies. Scale bar=500 μm. hESC: human embryonic stem cell, hiPSC: human induced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.
Article Snippet: The primary antibodies used in this study were as follows: anti-NESTIN (1:500; BioLegend), anti-SOX2 (1:1,000; Sigma-Aldrich), anti-ZO1 (1:50; Thermo Fisher Scientific), anti-PAX6 (1:200; Novus Biologicals), anti-TUJ1 (1:2,000; BioLegend), anti-TH (1:2,000; Pel-Freez), anti-glial fibrillary acidic protein (GFAP, 1:2,000; DAKO), anti-OLIG2 antibody (1:1,000; Chemicon), anti-major histocompatibility complex (MHC) Class I+HLA A/B (1:200, MHC-I; Abcam), anti-MHC Class II (1:200, MHC-II; Abcam).
Techniques: Marker, Staining, Activity Assay, Labeling, Reverse Transcription, Polymerase Chain Reaction, Expressing