Journal: Antioxidants
Article Title: Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells
doi: 10.3390/antiox10081314
Figure Lengend Snippet: Inhibitory effect of curcumin on B[a]P-induced AhR pathways. ( A ) HepG2 cells were stained for AhR (red). DAPI was used to stain the nuclei (blue). Objective: C-Apochromat 40 ×/ 1.20 W Korr M27. Pinholes: 1 AU for DAPI and AhR. Optical section thickness: 1.2 µm for DAPI and AhR. Modulation of the B[a]P-induced relative fluorescence intensities ( B ) and relative levels of AhR in nuclei ( C ) by curcumin treatment. N = 3 trials per sample and control. ( D ) Effect of curcumin on B[a]P-induced upregulation of CYP1A1 and CYP1B1. Relative expression levels of CYP1A1 ( E ) and CYP1B1 ( F ). N = 3 trials per sample and control. *** p < 0.001, compared with the control; ### p < 0.001, compared with the B[a]P-treated group. Con: control; B[a]P: benzo[a]pyrene (10 µM); C: curcumin (10 µM).
Article Snippet: Cyp1a1 and Cyp1b1 were knocked down using CYP1A1 and CYP1B1 siRNAs (Santa Cruz Biotechnology, Santa Cruz, CA, USA), respectively, by following the manufacturer’s protocol.
Techniques: Staining, Fluorescence, Expressing