Journal: Materials Today Bio

Article Title: Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications

doi: 10.1016/j.mtbio.2024.101059

Figure Lengend Snippet: Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Upon peroxidase (PO) detection with H 2 O 2 and 3,3′-diaminobenzidine (DAB, Sigma-Aldrich) for 10 min, sections were incubated with rabbit anti-rat CD86 (1:800; NBP2-67417, Bio-Techne, Abingdon, UK), overnight at 4 °C.

Techniques: Immunohistochemical staining, Marker, Staining, Clarification Assay