e dermatitidis strains atcc 44504  (ATCC)

Journal: eLife

Article Title: Binding and sequestration of poison frog alkaloids by a plasma globulin

doi: 10.7554/eLife.85096

Figure Lengend Snippet:

Article Snippet: Other , PTFE syringe filter , Thermo Scientific , Cat# 44504-NP , Consumable to filter samples prior to GC–MS analysis.

Techniques: Sequencing, Cell Culture, Expressing, Clinical Proteomics, Generated, Recombinant, Plasmid Preparation, Cloning, Purification, Mutagenesis, Labeling, RNA Sequencing, Produced, Software, Mass Spectrometry, Molecular Weight, Microscale Thermophoresis, Immunohistochemistry

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Survival of C. elegans after infection with E. dermatitidis strains from various sources. ( a ) Isolates from the environment (E); ( b ) Isolates from cystic fibrosis patients (CF); ( c ) Isolates from Asian patients (P). C. neoformans was used as an infection control; E. coli OP 50 was used as a non-infection control. E. dermatitidis exhibited pathogenicity in the C. elegans assay. Mortality rates varied between fungal strains. n = 30–40 individuals per test were used, every test was repeated 3 times.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection, Control

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Source and origin of included Exophiala dermatitidis strains.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Serial Time-encoded Amplified Microscopy, Mutagenesis

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Morphological analysis of E. dermatitidis in C. elegans after infection and incubation of infected nematodes for 72 h. Three separate pictures of E. dermatitidis development in C. elegans could be observed, leading to death: ( a ) Adherence of mainly fungal budding cell on the intestinal wall; proliferation and obstipation continued until no free intestinal structure could be observed, leading to death. ( b , c ) Invasive hyphal structures from inside the intestine to outside, leading to death. Scale = 400 µm.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection, Incubation

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Statistical evaluation of the morphology of E. dermatitidis in C. elegans during infection. Strains from cystic fibrosis patients (CF) strains exhibited no significant differences in morphological development after infection for 24 h or 48 h. Strains from Asian patients (P) exhibited significantly more hyphal formation after infection for 48 h than after infection for 24 h. Rates of nematodes were calculated with Kaplan-Meier statistics; comparison were performed with Mantel-Cox log-rank tests with statistical significance set at the level of P ≤ 0.05. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001, n = 24.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection, Comparison

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Survival of G. mellonella after infection with wild-type strains of E. dermatitidis from various sources: (a ) Isolates from the environment (E). ( b ) Isolates from cystic fibrosis patients (CF). ( c ) Isolates from systemic infections in patients from Asia (P). C. albicans was used as an infection control, sterile phosphate-buffered saline (PBS) was injected as a non-infection control, and a group of unharmed caterpillars were used as a quality control of the caterpillar population. All tested E. dermatitidis strains exhibited pathogenicity against G. mellonella caterpillars. n = 10 individuals per test were used, every test was repeated 3 times.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection, Control, Sterility, Saline, Injection

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Comparison of the mean survival curves of the three strain groups of E. dermatitidis isolates: isolates from cystic fibrosis patients (CF), isolates from Asian patients with systemic infections (P), and environmental isolates (E). P strains exhibited a significantly higher virulence against G. mellonella than E strains or CF strains. Curves were compared with the Mantel-Cox log-rank test with significance set at the level of P ≤ 0.05. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Comparison

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Kinetics of fungal proliferation in G. mellonella during infection. E. dermatitidis cells were recovered from infected G. mellonella caterpillars 2 h, 6 h, 24 h, 48 h, and 72 h after infection by CFU experiment (n = 18 strains, 6 from each group). Growth curve shows a sigmoidal development, all tested strains showed a similar growth rate over a period of 72 h. n = 2 G. mellonella caterpillars per test were used, every test was repeated 3 times.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Histopathologic evaluation of E. dermatitidis infection in G. mellonella . ( a ) No sign of immune system activity in the PBS control 72 h after infection. ( b ) Immune activity 48 h after infection. Haemocytes are creating massive capsule structures around fungal cells. Fungal cells of E. dermatitidis are still alive and the budding process is continuing. ( c ) Immune response against fungal cells also continues 72 h after infection. Slides were stained with hematoxylin and eosin (H&E) and were observed with a light microscope at a magnification of 400x (Hae = Haemocytes, T = Trachea, M = Muscle, F = Fett-Body, C = Capsule, Y = Yeast).

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Infection, Activity Assay, Control, Staining, Light Microscopy

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Histopathological analysis of E. dermatitidis in G. mellonella and comparison between a CF strain and a P strain infection. Shown is a typical strain from a cystic fibrosis (CF) patient with no real hyphal structures within the first 72 h after infection; pseudohyphal structures begin to develop within 48 h ( a ) 24 h after infection; ( c ) 48 h after infection; ( e ) 72 h after infection). A strain from an Asian patient (P) with a systemic infection shows the growth of true hyphal structures within 24 h after infection and a massive invasion of hyphae over a period of 72 h ( b . 24 h after infection; ( d) 48 h after infection; ( f ) 72 h after infection). All strains were observed with a light microscope at a magnification of 400x. Slides for histologic analysis were stained with Grocott silver. (H = Hyphae, P = Pseudohyphae, Y = Yeast).

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: Comparison, Infection, Light Microscopy, Staining

Journal: Scientific Reports

Article Title: Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms ( Caenorhabditis elegans and Galleria mellonella ) to determine virulence

doi: 10.1038/s41598-018-30909-5

Figure Lengend Snippet: Dendrogram of cluster analysis estimated by DiversiLab®. 18 E. dermatitidis isolates of various origins were analysed and clustered after Kullback-Leibler algorithm. Line indicates 95% similarity.

Article Snippet: Mel −3 , Exophiala dermatitidis , ATCC 44504 , Melanin-deficient mutant , ‡.

Techniques: