Review





Similar Products

hairpin stip1 shrna lentiviral particle gene silencer kit  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology hairpin stip1 shrna lentiviral particle gene silencer kit
( A ) After 24-h culture of the RCC tumor cells, secreted <t>STIP1</t> in the culture medium supernatant was detected, while the intracellular protein GAPDH was not detected in the culture medium supernatant indicating no leakage of intracellular components into the culture media. ( B – C ). STIP1 was detected in the purified cell surface protein, while no trace of HSP90 was identified, while HSP90 was detected in the total cell lysates (C). ( D ) Quantification of STIP1 protein in the culture medium supernatant as secreted STIP1 (left panel), and in the purified cell surface protein as outer cell surface STIP1 (right panel). Experiments were triplicated, and mean ± SD was presented. *p < 0.05, vs OS-RC-2; # p < 0.05, vs ACHN. In all panels, western blot images have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions.
Hairpin Stip1 Shrna Lentiviral Particle Gene Silencer Kit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hairpin stip1 shrna lentiviral particle gene silencer kit/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
hairpin stip1 shrna lentiviral particle gene silencer kit - by Bioz Stars, 2025-03
86/100 stars
  Buy from Supplier
statip1 sirna  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology statip1 sirna
Immunofluorescence analyses of STAT3, STAT3-Y and <t>STATIP1</t> proteins. STAT3, STAT3-Y and STATIP1 FITC-labeled antibodies (green), DAPI-stained DNA (blue) and merged images. Protein labeling was observed in untreated K562 cells (A-I) and K562 cells treated with 1 μM IM (J-R) . The slides were analyzed using an LMS confocal system, and the images were processed using AxioVision-LE software (Carl Zeiss).
Statip1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/statip1 sirna/product/Santa Cruz Biotechnology
Average 85 stars, based on 1 article reviews
statip1 sirna - by Bioz Stars, 2025-03
85/100 stars
  Buy from Supplier

Image Search Results


( A ) After 24-h culture of the RCC tumor cells, secreted STIP1 in the culture medium supernatant was detected, while the intracellular protein GAPDH was not detected in the culture medium supernatant indicating no leakage of intracellular components into the culture media. ( B – C ). STIP1 was detected in the purified cell surface protein, while no trace of HSP90 was identified, while HSP90 was detected in the total cell lysates (C). ( D ) Quantification of STIP1 protein in the culture medium supernatant as secreted STIP1 (left panel), and in the purified cell surface protein as outer cell surface STIP1 (right panel). Experiments were triplicated, and mean ± SD was presented. *p < 0.05, vs OS-RC-2; # p < 0.05, vs ACHN. In all panels, western blot images have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions.

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) After 24-h culture of the RCC tumor cells, secreted STIP1 in the culture medium supernatant was detected, while the intracellular protein GAPDH was not detected in the culture medium supernatant indicating no leakage of intracellular components into the culture media. ( B – C ). STIP1 was detected in the purified cell surface protein, while no trace of HSP90 was identified, while HSP90 was detected in the total cell lysates (C). ( D ) Quantification of STIP1 protein in the culture medium supernatant as secreted STIP1 (left panel), and in the purified cell surface protein as outer cell surface STIP1 (right panel). Experiments were triplicated, and mean ± SD was presented. *p < 0.05, vs OS-RC-2; # p < 0.05, vs ACHN. In all panels, western blot images have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions.

Article Snippet: Knockdown of STIP1 was achieved by a validated hairpin STIP1 shRNA Lentiviral Particle Gene Silencer kit (Santa Cruz Biotechnology, Texas, USA).

Techniques: Purification, Western Blot

( A ) STIP1 protein was examined in 19 protein specimens from primary RCC tumors (P, n = 7) and bone metastatic samples (M, n = 12). Proteins were electrophoresed in two 10% SDS-PAGE gels, and were subsequently transferred to two PVDF membranes (10 and 9 specimens for gels 1and 2, respectively). ( B ) The intensity of STIP1 in each lane was normalized with the intensity of GAPDH. *p < 0.05. Experiments were duplicated, and western blot images shown have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions. ( C ) Representative immunohistochemistry staining of STIP1 in primary RCC and bone metastasis tumors. Both intracellular and extracellular STIP1 immunoreactivity was examined as shown in the inset. Images were taken under 20× objective. Scale bar: 50 μm. ( D ) Correlation between the H scores of STIP1 in primary RCC and bone metastasis tumors of the 10 pairs of matched samples. R 2 = 0.6923.

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) STIP1 protein was examined in 19 protein specimens from primary RCC tumors (P, n = 7) and bone metastatic samples (M, n = 12). Proteins were electrophoresed in two 10% SDS-PAGE gels, and were subsequently transferred to two PVDF membranes (10 and 9 specimens for gels 1and 2, respectively). ( B ) The intensity of STIP1 in each lane was normalized with the intensity of GAPDH. *p < 0.05. Experiments were duplicated, and western blot images shown have been cropped to show the protein of interest, and all blots were performed under the same experimental conditions. ( C ) Representative immunohistochemistry staining of STIP1 in primary RCC and bone metastasis tumors. Both intracellular and extracellular STIP1 immunoreactivity was examined as shown in the inset. Images were taken under 20× objective. Scale bar: 50 μm. ( D ) Correlation between the H scores of STIP1 in primary RCC and bone metastasis tumors of the 10 pairs of matched samples. R 2 = 0.6923.

Article Snippet: Knockdown of STIP1 was achieved by a validated hairpin STIP1 shRNA Lentiviral Particle Gene Silencer kit (Santa Cruz Biotechnology, Texas, USA).

Techniques: SDS Page, Western Blot, Immunohistochemistry, Staining

( A ) STIP1 mRNA expressed highly in the advanced stage RCC tumors. ( B ) STIP1 mRNA expressed highly in the high grades RCC tumors. ( C ) STIP1 mRNA expressed highly in the metastatic RCC tumors (M1+). Overexpression gene rank and P value were generated by the Oncomine algorithms. Fold change was log 2 based.

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) STIP1 mRNA expressed highly in the advanced stage RCC tumors. ( B ) STIP1 mRNA expressed highly in the high grades RCC tumors. ( C ) STIP1 mRNA expressed highly in the metastatic RCC tumors (M1+). Overexpression gene rank and P value were generated by the Oncomine algorithms. Fold change was log 2 based.

Article Snippet: Knockdown of STIP1 was achieved by a validated hairpin STIP1 shRNA Lentiviral Particle Gene Silencer kit (Santa Cruz Biotechnology, Texas, USA).

Techniques: Over Expression, Generated

( A ) Proliferation of OS-RC-BM5 cells under indicated treatment. ( B ) Cell cycle analysis of OS-RC-BM5 cells under indicated treatment. ( C ) shRNA knockdown of STIP1 in OS-RC-BM5 cells. ( D ) Representative images of Ki67 immunoreactivity in the bone metastasis tumors. Images were taken under 20× objective. Scale bar: 50 μm. ( E ) Correlation between the H scores of STIP1 and percentage of Ki67-positive cells in the same bone metastasis tumors ( n =). R 2 = 0.5868.

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) Proliferation of OS-RC-BM5 cells under indicated treatment. ( B ) Cell cycle analysis of OS-RC-BM5 cells under indicated treatment. ( C ) shRNA knockdown of STIP1 in OS-RC-BM5 cells. ( D ) Representative images of Ki67 immunoreactivity in the bone metastasis tumors. Images were taken under 20× objective. Scale bar: 50 μm. ( E ) Correlation between the H scores of STIP1 and percentage of Ki67-positive cells in the same bone metastasis tumors ( n =). R 2 = 0.5868.

Article Snippet: Knockdown of STIP1 was achieved by a validated hairpin STIP1 shRNA Lentiviral Particle Gene Silencer kit (Santa Cruz Biotechnology, Texas, USA).

Techniques: Cell Cycle Assay, shRNA

( A ) Representative images of the Transwell membranes with tumor cells migrated to the counter side of the chamber. Note, cell migration was not affected when hrSTIP1 was added into the lower chamber (+ lower chamber). ( B ) Quantification of the migration analysis with three repeats. *p < 0.05, vs vehicle; # p < 0.05, vs hrSTIP1+anti-STIP1.

Journal: Oncotarget

Article Title: Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

doi: 10.18632/oncotarget.15222

Figure Lengend Snippet: ( A ) Representative images of the Transwell membranes with tumor cells migrated to the counter side of the chamber. Note, cell migration was not affected when hrSTIP1 was added into the lower chamber (+ lower chamber). ( B ) Quantification of the migration analysis with three repeats. *p < 0.05, vs vehicle; # p < 0.05, vs hrSTIP1+anti-STIP1.

Article Snippet: Knockdown of STIP1 was achieved by a validated hairpin STIP1 shRNA Lentiviral Particle Gene Silencer kit (Santa Cruz Biotechnology, Texas, USA).

Techniques: Migration

Immunofluorescence analyses of STAT3, STAT3-Y and STATIP1 proteins. STAT3, STAT3-Y and STATIP1 FITC-labeled antibodies (green), DAPI-stained DNA (blue) and merged images. Protein labeling was observed in untreated K562 cells (A-I) and K562 cells treated with 1 μM IM (J-R) . The slides were analyzed using an LMS confocal system, and the images were processed using AxioVision-LE software (Carl Zeiss).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: Immunofluorescence analyses of STAT3, STAT3-Y and STATIP1 proteins. STAT3, STAT3-Y and STATIP1 FITC-labeled antibodies (green), DAPI-stained DNA (blue) and merged images. Protein labeling was observed in untreated K562 cells (A-I) and K562 cells treated with 1 μM IM (J-R) . The slides were analyzed using an LMS confocal system, and the images were processed using AxioVision-LE software (Carl Zeiss).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: Immunofluorescence, Labeling, Staining, Software

Expression levels of STATIP1 , STAT3 , CCND1 and BCL-XL genes in response to IM/LLL-3 treatments. (A) Relative mRNA levels of STATIP1, STAT3, CCND1 and BCL-XL after 24 h of 1 μM IM treatment. (B) Relative mRNA levels of STATIP1, STAT3, CCND1 and BCL-XL after 24 h of 40 μM LLL-3 treatment. (C) Western blot analysis of STAT3 and STATIP1 protein levels and STAT3-Y705 phosphorylation 24 h after 1 μM IM treatment. (D) The protein levels were determined by densitometry analysis in ImageJ software version 1.44. All comparisons were made to untreated cells – ctrl. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: Expression levels of STATIP1 , STAT3 , CCND1 and BCL-XL genes in response to IM/LLL-3 treatments. (A) Relative mRNA levels of STATIP1, STAT3, CCND1 and BCL-XL after 24 h of 1 μM IM treatment. (B) Relative mRNA levels of STATIP1, STAT3, CCND1 and BCL-XL after 24 h of 40 μM LLL-3 treatment. (C) Western blot analysis of STAT3 and STATIP1 protein levels and STAT3-Y705 phosphorylation 24 h after 1 μM IM treatment. (D) The protein levels were determined by densitometry analysis in ImageJ software version 1.44. All comparisons were made to untreated cells – ctrl. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: Expressing, Western Blot, Software

STATIP1 mRNA depletion by siRNA induces the over-expression of STAT3 and its target genes. (A) STATIP1 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing, as determined by RT-qPCR. (B) Western blot analyses of the STATIP1 protein level 72 h after STATIP1 silencing. (C) RT-qPCR analyses of the STAT3 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing. (D) RT-qPCR analyses of the CCND1 and BCL-XL mRNA levels at 72 h after STATIP1 silencing. All comparisons were made to untreated cells – ctrl and scrambled-treated cells. Ctrl: control. The data represent the means ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: STATIP1 mRNA depletion by siRNA induces the over-expression of STAT3 and its target genes. (A) STATIP1 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing, as determined by RT-qPCR. (B) Western blot analyses of the STATIP1 protein level 72 h after STATIP1 silencing. (C) RT-qPCR analyses of the STAT3 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing. (D) RT-qPCR analyses of the CCND1 and BCL-XL mRNA levels at 72 h after STATIP1 silencing. All comparisons were made to untreated cells – ctrl and scrambled-treated cells. Ctrl: control. The data represent the means ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: Over Expression, Quantitative RT-PCR, Western Blot

Involvement of STATIP1 and STAT3 genes in IM resistance in CML cell lines. (A) STATIP1 mRNA levels in K562 and Lucena cells determined by RT-qPCR. (B) STAT3 mRNA levels, as determined by RT-qPCR, in Lucena cells under the following conditions: 1 μM IM treatment, 40 μM LLL-3 treatment, and co-treatment after 24 h. (C) The ABCB1 mRNA levels in Lucena cells were determined by RT-qPCR under the treatment conditions noted above. (D) Apoptotic cells were measured by flow cytometry in both cell lines under the treatment conditions noted above. The cell cycle was evaluated by flow cytometry after being subjected to the treatment conditions noted above in K562 (E) and Lucena (F) cells. All comparisons were made to untreated cells – ctrl. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: Involvement of STATIP1 and STAT3 genes in IM resistance in CML cell lines. (A) STATIP1 mRNA levels in K562 and Lucena cells determined by RT-qPCR. (B) STAT3 mRNA levels, as determined by RT-qPCR, in Lucena cells under the following conditions: 1 μM IM treatment, 40 μM LLL-3 treatment, and co-treatment after 24 h. (C) The ABCB1 mRNA levels in Lucena cells were determined by RT-qPCR under the treatment conditions noted above. (D) Apoptotic cells were measured by flow cytometry in both cell lines under the treatment conditions noted above. The cell cycle was evaluated by flow cytometry after being subjected to the treatment conditions noted above in K562 (E) and Lucena (F) cells. All comparisons were made to untreated cells – ctrl. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: Quantitative RT-PCR, Flow Cytometry

Evaluation of cell viability in STATIP1-silenced K562 cells after IM treatment. The relative percentage of viable cells was determined by WST-1 assay after 24 h of IM treatment (+), compared to untreated cells – ctrl and scrambled-treated cells. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: Evaluation of cell viability in STATIP1-silenced K562 cells after IM treatment. The relative percentage of viable cells was determined by WST-1 assay after 24 h of IM treatment (+), compared to untreated cells – ctrl and scrambled-treated cells. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: WST-1 Assay

Expression levels of STATIP1 and STAT3 genes in CML patients. (A) STATIP1 mRNA levels and (B) STAT3 mRNA levels were determined by RT-qPCR analyses in 6 IM-responsive patients and 8 IM-resistant patients. Raw expression values were normalized to β-actin expression. Expression changes were calibrated by 6 healthy bone marrow donors analysis. Resp. P = responsive patients; Resist. P. = resistant patients. (*p <0.05).

Journal: BMC Cancer

Article Title: Inhibition of STAT3-interacting protein 1 (STATIP1) promotes STAT3 transcriptional up-regulation and imatinib mesylate resistance in the chronic myeloid leukemia

doi: 10.1186/1471-2407-14-866

Figure Lengend Snippet: Expression levels of STATIP1 and STAT3 genes in CML patients. (A) STATIP1 mRNA levels and (B) STAT3 mRNA levels were determined by RT-qPCR analyses in 6 IM-responsive patients and 8 IM-resistant patients. Raw expression values were normalized to β-actin expression. Expression changes were calibrated by 6 healthy bone marrow donors analysis. Resp. P = responsive patients; Resist. P. = resistant patients. (*p <0.05).

Article Snippet: STATIP1 siRNA (100 nM) (SC-44436, Santa Cruz) and 2 μL of Lipofectamine™ RNAiMAX (Invitrogen) were incubated separately in a final volume of 50 μL of RPMI-1640 media for 5 min.

Techniques: Expressing, Quantitative RT-PCR