Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Micro-RNA 205-5p is Involved in the Progression of Gastric Cancer and Targets Phosphatase and Tensin Homolog (PTEN) in SGC-7901 Human Gastric Cancer Cells

doi: 10.12659/MSM.915970

Figure Lengend Snippet: Histology of gastric cancer tissue and adjacent normal gastric tissue, PTEN protein expression, and expression of micro-RNA 205-5p (miR-205-5p). ( A ) Adjacent normal gastric tissue, gastric cancer tissue from patients with stage I–II, and stage III–IV gastric cancer. Hematoxylin and eosin (H&E). Magnification, ×100 or ×400. ( B ) miR-205-5p expression by in situ hybridization. Magnification, ×100 or ×400. ** P<0.01 and *** P<0.001 compared with normal; ## P<0.01 compared with stage I–II. ( C ) PTEN protein expression by immunohistochemistry. Magnification, ×100 or ×400. ** P<0.01 and *** P<0.001 compared with Normal; ## P<0.01, compared with stage I–II.

Article Snippet: PTEN short interfering RNA (siRNA) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and thiazolyl blue 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), the Annexin-V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) apoptosis detection kit and the polyvinylidene fluoride (PVDF) membrane were obtained from Sigma-Aldrich (St. Louis MO, USA).

Techniques: Expressing, In Situ Hybridization, Immunohistochemistry