Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 3. Time course of toluene concentrations in assays conducted with C. vulgaris, R. opacus or a consortium of these microorganisms with nitrate or a combination of ammonium and nitrate as nitrogen sources. Initial concentra tion of toluene was 3 mg L−1 in all the conditions.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 4. Time course of (A) phenol, (B) benzene, (C) toluene, (D) ethylbenzene and (E) o-xylene concentrations in C. vulgaris-R. opacus co-cultures under an air atmosphere.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 5. Time course of phenol and BTEX concentrations in (A) Mix 1, (B) Mix 2 and (C) Mix 3 in C. vulgaris-R. opacus cultures cultivated with in a mixture of the five pollutants under air atmosphere. Initial concentrations of the pollutants (100 %) in the mixture were 25 mg L−1 of phenol, and 3, 3, 8 mg L−1 for benzene and toluene, 1, 3, 3 mg L−1 for ethylbenzene; and 0.5, 1, 1 mg L−1 for o-xylene in Mix 1, 2 and 3, respectively.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 6. Time course of (A) phenol, (B) benzene, (C) toluene, (D) ethylbenzene and (E) o-xylene concentration in C. vulgaris-R. opacus cultures under a N2/CO2 (70/30 %) atmosphere.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques: Concentration Assay

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 7. Time course of phenol and BTEX concentration in (A) Mix 1, (B) Mix 2 and (C) Mix 3 in C. vulgaris-R. opacus cultures incubated with a mixture of the five pollutants under a N2/CO2 (70/30 %) atmosphere. Initial concentrations of the pollutants (100 %) in the mixture were 25 mg L−1 of phenol, and 3, 3, 8 mg L−1 for benzene and toluene, 1, 3, 3 mg L−1 for ethylbenzene; and 0.5, 1, 1 mg L−1 for o-xylene in Mix 1, 2 and 3, respectively.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques: Concentration Assay, Incubation

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators

doi: 10.1186/s13068-024-02523-3

Figure Lengend Snippet: The PaaX-like TR 13 induces carbon-specific alternate transcriptional programs and represses phenylacetic acid degradation. a Heatmap visualizing the differential expression of KEGG pathways. Each mutant was compared to the WT strain grown using the same carbon source for differential expression in all the R. opacus ’ annotated KEGG pathways (rows). The color of each cell of the heatmap denotes the fold change versus WT, and white cells represent non-significant changes. Each column represents the DE of one condition, averaged from replicates and tested using GAGE in R. Annotation bars on top of the heatmap denote the strain and carbon source in each column, and the row annotation bar on the side denotes the BRITE functional hierarchy classification for each pathway. Rows are clustered using the “complete” distance method. b Heatmap visualizing the DE of the phenylacetic acid degradation pathway in Strain 13 versus WT grown on phenol. Here, all loci annotated to the phenylacetic acid pathway were shown as rows, and each column represents one of the replicates for each condition. Column annotation bars on top of the metric indicate the strain and carbon source designation of each replicate. Rows and columns are clustered using Euclidean distance and the “complete” clustering metric. Heatmap values are regularized-log transformed expression counts

Article Snippet: The ancestral, or wild-type (WT), strain for all transformant cells was Rhodococcus opacus PD630 (DSMZ 44193).

Techniques: Quantitative Proteomics, Mutagenesis, Functional Assay, Transformation Assay, Expressing

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: (A) The wild or mutant sequences of Myd88. (B) Luciferase reporter gene analysis validate the relationship of miR-525-5p and Myd88. (C and D) Myd88 mRNA and protein expression was detected by RT-qPCR and Western blotting. (E) Myd88 protein expression. (F) The mRNA expression of Myd88 in lymphoid cancer cell lines (OCl-LY7, FARAGE and U2932) and human lymphoblastoid B cells (GM12878). N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Mutagenesis, Luciferase, Expressing, Quantitative RT-PCR, Western Blot

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: The U2932 cells were transfected with Myd88 overexpression vector (OE-Myd88) or Myd88 siRNA (si-Myd88) for 24 h, respectively. (A) Myd88 protein expression was detected by Western blotting. (B) CCK-8 assay was used to detect cell proliferation. (C) Cell invasion was detected by Transwell invasion assay. (D) Cell apoptosis was detected by flow cytometry. (E) Cell clonogenic capacity was detected by cell colony formation assay. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, Western Blot, CCK-8 Assay, Transwell Invasion Assay, Flow Cytometry, Colony Assay

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: The U2932 cells were transfected with miR-525-5p mimic alone or together with Myd88 overexpression vector. (A) The protein expression of Myd88 and NF-κB. (B) CCK-8 assay was used to detect cell proliferation. (C) Cell invasion was detected by Transwell invasion assay. (D) Cell apoptosis was detected by flow cytometry. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, CCK-8 Assay, Transwell Invasion Assay, Flow Cytometry

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: A total of 10 adult nude mice (4–6 weeks; 18–22 g; Wuhan Experimental Animal Center; Wuhan, China) were randomly divided into two groups ( N = 5 per group) including NC mimic group and miR-525-5p mimic group. A total of 10 adult nude mice were randomly divided into two groups ( N = 5 per group) including NC mimic group and miR-525-5p mimic group. (A) Tumor images on the 35th day after injection. (B) Tumor volume. (C) Tumor weight. (D) MiR-525-5p expression. (E) Myd88 and NF-κB protein expression. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Injection, Expressing