Journal: iScience

Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading

doi: 10.1016/j.isci.2025.112273

Figure Lengend Snippet:

Article Snippet: siIntegrin b1 , Santa Cruz , sc-35674.

Techniques: Recombinant, Selection, Staining, Negative Control, Software

Journal: Frontiers in Oncology

Article Title: Combined score of Y chromosome loss and T-cell infiltration improves UICC based stratification of esophageal adenocarcinoma

doi: 10.3389/fonc.2023.1249172

Figure Lengend Snippet: Marker: analyzable TMAs (n), mean of marker expression between 0 (negative) and 1 (positive), p value and hazard ratio of univariate Cox regression of the marker and overall survival.

Article Snippet: The TMA slides were stained with the following antibodies (clone, buffer, dilution, manufacturer) or FISH probes (probe name, manufacturer): AKR1 (EPR14421, EDTA, 1:500, abcam, UK), ARID domain-containing protein 1A (EPR13501, EDTA, 1:1000, abcam, UK), BRM (D9E8B, EDTA, 1:50, Cell Signaling Technology, MA, USA), BRG1 (EPNCIR111A, EDTA, 1:300, abcam, UK), Carbonic anhydrase IX (EPR4151, EDTA, 1:100, abcam, UK), CD3 (SP7, citrate, 1:50, Thermo Fisher Scientific, MA, USA), CD34 quantity (QB End10, citrate, 1:700, Cell Marque, CA, USA), CD36 (D8L9T, citrate, 1:200, Cell Signaling Technology, MA, USA), CD66b (G10F5, EDTA, 1:200, Novus Biologicals, CO, USA), CDKN2A/p16 (ZytoLight ® SPEC CDKN2A/CEN 9 Dual Color Probe, Zytomed, Germany), Claudin-18 (ERP19202, EDTA, 1:200, abcam, UK), c-Myc (Y69, citrate, 1:100, abcam, UK), Cyclin D1 (ZM178, citrate, 1:400, Zeta Corporation, CA, USA), E-Cadherin (M3612, EDTA, 1:50, Dako, CA, USA), FBP1 (EPR4619, EDTA, 1:100, abcam, UK), FBXW7 (SP237, EDTA, 1:500, abcam, UK), GATA-6 (GATA6-20-GR Probe, Empire Genomics, NY, USA), Grem1 (ab22138, EDTA, 1:400, abcam, UK), H3K27m3 (C36B11, EDTA, 1:100, Cell Signaling Technology, MA, USA), HER2 (4b5, EDTA, not diluted, Roche, Switzerland), HMGB1 (D3E5, EDTA, 1:500, Cell Signaling Technology, MA, USA), IMP3 (M3626, EDTA, 1:100, Agilent, Dako, CA, USA), ini1 (BCIR1, EDTA, 1:50, Zytomed Systems, Germany), Integrin alpha-5 (EPR7854, EDTA, 1:300, abcam, UK), Integrin beta-1 (A4, EDTA, 1:100, Santa Cruz Biotechnology, Germany), Integrin beta-4 (D8P6C, EDTA, 1:100, Cell Signaling Technology, MA, USA), Ki-67 (SP6, EDTA, 1:100, Cell Marque, CA, USA), KRas (9.13, citrate, 1:100, Thermo Fisher Scientific, MA, USA), LAG-3 (D2G40, EDTA, 1:300, Cell Signaling Technology, MA, USA), Mdm2 (Ab-1/IF2, EDTA, 1:50, Calbiochem, NJ, USA), Mesothelin (5B2, EDTA; 1:50, Novocastra, Switzerland), MET (SP44, EDTA, not diluted, Roche, Switzerland), MHC-1 (EPR1394Y, citrate, 1:300, abcam, UK), Mlh1 (M1, EDTA, not diluted, Roche, Switzerland), NOPE-Ca (RNAscope ® 2.5 LS Probe- Hs-IGDCC4, ACD, CA, USA), p53 (DO-7, citrate, 1:800, Dako, CA, USA), PD-L1 (E1L3N, EDTA, 1:400, Cell Signaling Technology, MA, USA), PIK3CA (6D9, EDTA, 1:1000, abnova, Taiwan), PTEN (138 G 6, EDTA, 1:300, Cell Signaling Technology, MA, USA), TIM-3 (D5D5R, EDTA, 1:100, Cell Signaling Technology, MA, USA), TROP-2 (ERP20043, EDTA, 1:1000, abcam, UK), UBQLN4 (RNAscope ® 2.5 LS Probe- Hs-UBQLN4, ACD, CA, USA), XIAP (ab21278, citrate, 1:1000, abcam, UK), preserved Y chromosome (long and short arm) (Vysis LSI SRY Spectrum Orange Probe and Vysis CEP Y (DYZ1) Spectrum Green Probe, Abbott Molecular, Germany).

Techniques: Marker, Expressing